Molecular detection and genotypic characterization of Toxoplasma gondii infection in bats in four provinces of China
Abstract Background Toxoplasma gondii is an intracellular protozoan parasite that infects a wide variety of warm-blooded hosts, including humans. Limited information about T. gondii infection in bats is available in China. The objective of the present study was to determine prevalence and genetic ch...
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| Format: | Article |
| Language: | English |
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BMC
2014-12-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13071-014-0558-7 |
| _version_ | 1797811661593116672 |
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| author | Si-Yuan Qin Wei Cong Ye Liu Nan Li Ze-Dong Wang Fu-Kai Zhang Si-Yang Huang Xing-Quan Zhu Quan Liu |
| author_facet | Si-Yuan Qin Wei Cong Ye Liu Nan Li Ze-Dong Wang Fu-Kai Zhang Si-Yang Huang Xing-Quan Zhu Quan Liu |
| author_sort | Si-Yuan Qin |
| collection | DOAJ |
| description | Abstract Background Toxoplasma gondii is an intracellular protozoan parasite that infects a wide variety of warm-blooded hosts, including humans. Limited information about T. gondii infection in bats is available in China. The objective of the present study was to determine prevalence and genetic characterization of T. gondii infection in bats in Jilin, Liaoning, Jiangxi and Guangdong provinces, China. Methods During May 2005 to August 2013, bats were sampled from Jilin, Liaoning, Jiangxi, and Guangdong provinces, China, and liver tissues were collected for the detection of T. gondii by a nested PCR targeting the B1 gene. The positive samples were genotyped at 11 genetic markers (SAG1, 5′-and 3′-SAG2, alternative SAG2, SAG3, BTUB, GRA6, L358, PK1, c22-8, c29-2, and Apico) using multilocus polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results A total of 626 bats representing 10 species were examined for T. gondii infection, 38 (6.1%) were tested positive with by PCR, 8 positive DNA samples were completely genotyped, of which 3 samples (2 from Cynopterus sphinx, and 1 from Murina leucogaster) represented ToxoDB#10, and 5 samples (2 from Murina leucogaster, 2 from Myotis chinensis, and 1 from Rhinolophus ferrumequinum) belonged to ToxoDB#9 ( http://toxodb.org/toxo/ ). Conclusions The present study revealed an overall T. gondii prevalence of 6.1% in bats from Jilin, Liaoning, Jiangxi and Guangdong provinces in China, and reported two T. gondii genotypes (ToxoDB#9 and #10) having a wide geographical distribution in China. These results provide new genetic information about T. gondii infection in bats, and have implications for better understanding of the genetic diversity of T. gondii in China and elsewhere. |
| first_indexed | 2024-03-13T07:27:00Z |
| format | Article |
| id | doaj.art-0ef65ffda2e94add90475c185e2ec306 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-03-13T07:27:00Z |
| publishDate | 2014-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-0ef65ffda2e94add90475c185e2ec3062023-06-04T11:19:13ZengBMCParasites & Vectors1756-33052014-12-01711510.1186/s13071-014-0558-7Molecular detection and genotypic characterization of Toxoplasma gondii infection in bats in four provinces of ChinaSi-Yuan Qin0Wei Cong1Ye Liu2Nan Li3Ze-Dong Wang4Fu-Kai Zhang5Si-Yang Huang6Xing-Quan Zhu7Quan Liu8State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesMilitary Veterinary Institute, Academy of Military Medical Sciences, Key Laboratory of Jilin Province for Zoonosis Prevention and ControlMilitary Veterinary Institute, Academy of Military Medical Sciences, Key Laboratory of Jilin Province for Zoonosis Prevention and ControlMilitary Veterinary Institute, Academy of Military Medical Sciences, Key Laboratory of Jilin Province for Zoonosis Prevention and ControlState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural SciencesMilitary Veterinary Institute, Academy of Military Medical Sciences, Key Laboratory of Jilin Province for Zoonosis Prevention and ControlAbstract Background Toxoplasma gondii is an intracellular protozoan parasite that infects a wide variety of warm-blooded hosts, including humans. Limited information about T. gondii infection in bats is available in China. The objective of the present study was to determine prevalence and genetic characterization of T. gondii infection in bats in Jilin, Liaoning, Jiangxi and Guangdong provinces, China. Methods During May 2005 to August 2013, bats were sampled from Jilin, Liaoning, Jiangxi, and Guangdong provinces, China, and liver tissues were collected for the detection of T. gondii by a nested PCR targeting the B1 gene. The positive samples were genotyped at 11 genetic markers (SAG1, 5′-and 3′-SAG2, alternative SAG2, SAG3, BTUB, GRA6, L358, PK1, c22-8, c29-2, and Apico) using multilocus polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results A total of 626 bats representing 10 species were examined for T. gondii infection, 38 (6.1%) were tested positive with by PCR, 8 positive DNA samples were completely genotyped, of which 3 samples (2 from Cynopterus sphinx, and 1 from Murina leucogaster) represented ToxoDB#10, and 5 samples (2 from Murina leucogaster, 2 from Myotis chinensis, and 1 from Rhinolophus ferrumequinum) belonged to ToxoDB#9 ( http://toxodb.org/toxo/ ). Conclusions The present study revealed an overall T. gondii prevalence of 6.1% in bats from Jilin, Liaoning, Jiangxi and Guangdong provinces in China, and reported two T. gondii genotypes (ToxoDB#9 and #10) having a wide geographical distribution in China. These results provide new genetic information about T. gondii infection in bats, and have implications for better understanding of the genetic diversity of T. gondii in China and elsewhere.https://doi.org/10.1186/s13071-014-0558-7Toxoplasma gondiiBatsPrevalenceGenetic characterizationChina |
| spellingShingle | Si-Yuan Qin Wei Cong Ye Liu Nan Li Ze-Dong Wang Fu-Kai Zhang Si-Yang Huang Xing-Quan Zhu Quan Liu Molecular detection and genotypic characterization of Toxoplasma gondii infection in bats in four provinces of China Parasites & Vectors Toxoplasma gondii Bats Prevalence Genetic characterization China |
| title | Molecular detection and genotypic characterization of Toxoplasma gondii infection in bats in four provinces of China |
| title_full | Molecular detection and genotypic characterization of Toxoplasma gondii infection in bats in four provinces of China |
| title_fullStr | Molecular detection and genotypic characterization of Toxoplasma gondii infection in bats in four provinces of China |
| title_full_unstemmed | Molecular detection and genotypic characterization of Toxoplasma gondii infection in bats in four provinces of China |
| title_short | Molecular detection and genotypic characterization of Toxoplasma gondii infection in bats in four provinces of China |
| title_sort | molecular detection and genotypic characterization of toxoplasma gondii infection in bats in four provinces of china |
| topic | Toxoplasma gondii Bats Prevalence Genetic characterization China |
| url | https://doi.org/10.1186/s13071-014-0558-7 |
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