Long acting β2-agonist and corticosteroid restore airway glandular cell function altered by bacterial supernatant
<p>Abstract</p> <p>Background</p> <p><it>Staphylococcus aureus </it>releases virulence factors (VF) that may impair the innate protective functions of airway cells. The aim of this study was to determine whether a long-acting β<sub>2 </sub>adrene...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2010-01-01
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| Series: | Respiratory Research |
| Online Access: | http://respiratory-research.com/content/11/1/6 |
| _version_ | 1811244676900978688 |
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| author | Nawrocki-Raby Béatrice Toumi Férial Delavoie Franck Zahm Jean-Marie Kileztky Claire Michel Jean Balossier Gérard Johnson Malcolm Coraux Christelle Birembaut Philippe |
| author_facet | Nawrocki-Raby Béatrice Toumi Férial Delavoie Franck Zahm Jean-Marie Kileztky Claire Michel Jean Balossier Gérard Johnson Malcolm Coraux Christelle Birembaut Philippe |
| author_sort | Nawrocki-Raby Béatrice |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p><it>Staphylococcus aureus </it>releases virulence factors (VF) that may impair the innate protective functions of airway cells. The aim of this study was to determine whether a long-acting β<sub>2 </sub>adrenergic receptor agonist (salmeterol hydroxynaphthoate, Sal) combined with a corticosteroid (fluticasone propionate, FP) was able to regulate ion content and cytokine expression by airway glandular cells after exposure to <it>S. aureus </it>supernatant.</p> <p>Methods</p> <p>A human airway glandular cell line was incubated with <it>S. aureus </it>supernatant for 1 h and then treated with the combination Sal/FP for 4 h. The expression of actin and CFTR proteins was analyzed by immunofluorescence. Videomicroscopy was used to evaluate chloride secretion and X-ray microanalysis to measure the intracellular ion and water content. The pro-inflammatory cytokine expression was assessed by RT-PCR and ELISA.</p> <p>Results</p> <p>When the cells were incubated with <it>S. aureus </it>supernatant and then with Sal/FP, the cellular localisation of CFTR was apical compared to the cytoplasmic localisation in cells incubated with <it>S. aureus </it>supernatant alone. The incubation of airway epithelial cells with <it>S. aureus </it>supernatant reduced by 66% the chloride efflux that was fully restored by Sal/FP treatment. We also observed that Sal/FP treatment induced the restoration of ion (Cl and S) and water content within the intracellular secretory granules of airway glandular cells and reduced the bacterial supernatant-dependent increase of pro-inflammatory cytokines IL8 and TNFα.</p> <p>Conclusions</p> <p>Our results demonstrate that treatment with the combination of a corticosteroid and a long-acting β<sub>2 </sub>adrenergic receptor agonist after bacterial infection restores the airway glandular cell function. Abnormal mucus induced by defective ion transport during pulmonary infection could benefit from treatment with a combination of β<sub>2 </sub>adrenergic receptor agonist and glucocorticoid.</p> |
| first_indexed | 2024-04-12T14:29:25Z |
| format | Article |
| id | doaj.art-0efebf315a6648fc9a65f7c90291cf41 |
| institution | Directory Open Access Journal |
| issn | 1465-9921 |
| language | English |
| last_indexed | 2024-04-12T14:29:25Z |
| publishDate | 2010-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Respiratory Research |
| spelling | doaj.art-0efebf315a6648fc9a65f7c90291cf412022-12-22T03:29:21ZengBMCRespiratory Research1465-99212010-01-01111610.1186/1465-9921-11-6Long acting β2-agonist and corticosteroid restore airway glandular cell function altered by bacterial supernatantNawrocki-Raby BéatriceToumi FérialDelavoie FranckZahm Jean-MarieKileztky ClaireMichel JeanBalossier GérardJohnson MalcolmCoraux ChristelleBirembaut Philippe<p>Abstract</p> <p>Background</p> <p><it>Staphylococcus aureus </it>releases virulence factors (VF) that may impair the innate protective functions of airway cells. The aim of this study was to determine whether a long-acting β<sub>2 </sub>adrenergic receptor agonist (salmeterol hydroxynaphthoate, Sal) combined with a corticosteroid (fluticasone propionate, FP) was able to regulate ion content and cytokine expression by airway glandular cells after exposure to <it>S. aureus </it>supernatant.</p> <p>Methods</p> <p>A human airway glandular cell line was incubated with <it>S. aureus </it>supernatant for 1 h and then treated with the combination Sal/FP for 4 h. The expression of actin and CFTR proteins was analyzed by immunofluorescence. Videomicroscopy was used to evaluate chloride secretion and X-ray microanalysis to measure the intracellular ion and water content. The pro-inflammatory cytokine expression was assessed by RT-PCR and ELISA.</p> <p>Results</p> <p>When the cells were incubated with <it>S. aureus </it>supernatant and then with Sal/FP, the cellular localisation of CFTR was apical compared to the cytoplasmic localisation in cells incubated with <it>S. aureus </it>supernatant alone. The incubation of airway epithelial cells with <it>S. aureus </it>supernatant reduced by 66% the chloride efflux that was fully restored by Sal/FP treatment. We also observed that Sal/FP treatment induced the restoration of ion (Cl and S) and water content within the intracellular secretory granules of airway glandular cells and reduced the bacterial supernatant-dependent increase of pro-inflammatory cytokines IL8 and TNFα.</p> <p>Conclusions</p> <p>Our results demonstrate that treatment with the combination of a corticosteroid and a long-acting β<sub>2 </sub>adrenergic receptor agonist after bacterial infection restores the airway glandular cell function. Abnormal mucus induced by defective ion transport during pulmonary infection could benefit from treatment with a combination of β<sub>2 </sub>adrenergic receptor agonist and glucocorticoid.</p>http://respiratory-research.com/content/11/1/6 |
| spellingShingle | Nawrocki-Raby Béatrice Toumi Férial Delavoie Franck Zahm Jean-Marie Kileztky Claire Michel Jean Balossier Gérard Johnson Malcolm Coraux Christelle Birembaut Philippe Long acting β2-agonist and corticosteroid restore airway glandular cell function altered by bacterial supernatant Respiratory Research |
| title | Long acting β2-agonist and corticosteroid restore airway glandular cell function altered by bacterial supernatant |
| title_full | Long acting β2-agonist and corticosteroid restore airway glandular cell function altered by bacterial supernatant |
| title_fullStr | Long acting β2-agonist and corticosteroid restore airway glandular cell function altered by bacterial supernatant |
| title_full_unstemmed | Long acting β2-agonist and corticosteroid restore airway glandular cell function altered by bacterial supernatant |
| title_short | Long acting β2-agonist and corticosteroid restore airway glandular cell function altered by bacterial supernatant |
| title_sort | long acting β2 agonist and corticosteroid restore airway glandular cell function altered by bacterial supernatant |
| url | http://respiratory-research.com/content/11/1/6 |
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