Effect of Melatonin on the <i>In Vitro</i> Maturation of Porcine Oocytes, Development of Parthenogenetically Activated Embryos, and Expression of Genes Related to the Oocyte Developmental Capability
Melatonin treatment can improve quality and in vitro development of porcine oocytes, but the mechanism of improving quality and developmental competence is not fully understood. In this study, porcine cumulus−oocyte complexes were cultured in TCM199 medium with non-treated (control), 10<...
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MDPI AG
2020-01-01
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| Online Access: | https://www.mdpi.com/2076-2615/10/2/209 |
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| author | Ling Yang Qingkai Wang Maosheng Cui Qianjun Li Shuqin Mu Zimo Zhao |
| author_facet | Ling Yang Qingkai Wang Maosheng Cui Qianjun Li Shuqin Mu Zimo Zhao |
| author_sort | Ling Yang |
| collection | DOAJ |
| description | Melatonin treatment can improve quality and in vitro development of porcine oocytes, but the mechanism of improving quality and developmental competence is not fully understood. In this study, porcine cumulus−oocyte complexes were cultured in TCM199 medium with non-treated (control), 10<sup>−5</sup> M luzindole (melatonin receptor antagonist), 10<sup>−5</sup> M melatonin, and melatonin + luzindole during in vitro maturation, and parthenogenetically activated (PA) embryos were treated with nothing (control), or 10<sup>−5</sup> M melatonin. Cumulus oophorus expansion, oocyte survival rate, first polar body extrusion rate, mitochondrial distribution, and intracellular levels of reactive oxygen species (ROS) and glutathione of oocytes, and cleavage rate and blastocyst rate of the PA embryos were assessed. In addition, expression of growth differentiation factor 9 (GDF9), tumor protein p53 (P53), BCL2 associated X protein (BAX), catalase (CAT), and bone morphogenetic protein 15 (BMP15) were analyzed by real-time quantitative PCR. The results revealed that melatonin treatment not only improved the first polar body extrusion rate and cumulus expansion of oocytes via melatonin receptors, but also enhanced the rates of cleavage and blastocyst formation of PA embryos. Additionally, melatonin treatment significantly increased intraooplasmic level of glutathione independently of melatonin receptors. Furthermore, melatonin supplementation not only significantly enhanced mitochondrial distribution and relative abundances of <i>BMP15</i> and <i>CAT</i> mRNA, but also decreased intracellular level of ROS and relative abundances of <i>P53</i> and <i>BAX</i> mRNA of the oocytes. In conclusion, melatonin enhanced the quality and in vitro development of porcine oocytes, which may be related to antioxidant and anti-apoptotic mechanisms. |
| first_indexed | 2024-12-19T06:41:59Z |
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| language | English |
| last_indexed | 2024-12-19T06:41:59Z |
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| spelling | doaj.art-0f0433021475430cb7502d8118fb14b92022-12-21T20:32:02ZengMDPI AGAnimals2076-26152020-01-0110220910.3390/ani10020209ani10020209Effect of Melatonin on the <i>In Vitro</i> Maturation of Porcine Oocytes, Development of Parthenogenetically Activated Embryos, and Expression of Genes Related to the Oocyte Developmental CapabilityLing Yang0Qingkai Wang1Maosheng Cui2Qianjun Li3Shuqin Mu4Zimo Zhao5College of Life Sciences and Food Engineering, Hebei University of Engineering, Handan 056021, ChinaCollege of Life Sciences and Food Engineering, Hebei University of Engineering, Handan 056021, ChinaAnimal Husbandry and Veterinary Research Institute of Tianjin, Tianjin 300412, ChinaAnimal Husbandry and Veterinary Research Institute of Tianjin, Tianjin 300412, ChinaAnimal Husbandry and Veterinary Research Institute of Tianjin, Tianjin 300412, ChinaCollege of Life Sciences and Food Engineering, Hebei University of Engineering, Handan 056021, ChinaMelatonin treatment can improve quality and in vitro development of porcine oocytes, but the mechanism of improving quality and developmental competence is not fully understood. In this study, porcine cumulus−oocyte complexes were cultured in TCM199 medium with non-treated (control), 10<sup>−5</sup> M luzindole (melatonin receptor antagonist), 10<sup>−5</sup> M melatonin, and melatonin + luzindole during in vitro maturation, and parthenogenetically activated (PA) embryos were treated with nothing (control), or 10<sup>−5</sup> M melatonin. Cumulus oophorus expansion, oocyte survival rate, first polar body extrusion rate, mitochondrial distribution, and intracellular levels of reactive oxygen species (ROS) and glutathione of oocytes, and cleavage rate and blastocyst rate of the PA embryos were assessed. In addition, expression of growth differentiation factor 9 (GDF9), tumor protein p53 (P53), BCL2 associated X protein (BAX), catalase (CAT), and bone morphogenetic protein 15 (BMP15) were analyzed by real-time quantitative PCR. The results revealed that melatonin treatment not only improved the first polar body extrusion rate and cumulus expansion of oocytes via melatonin receptors, but also enhanced the rates of cleavage and blastocyst formation of PA embryos. Additionally, melatonin treatment significantly increased intraooplasmic level of glutathione independently of melatonin receptors. Furthermore, melatonin supplementation not only significantly enhanced mitochondrial distribution and relative abundances of <i>BMP15</i> and <i>CAT</i> mRNA, but also decreased intracellular level of ROS and relative abundances of <i>P53</i> and <i>BAX</i> mRNA of the oocytes. In conclusion, melatonin enhanced the quality and in vitro development of porcine oocytes, which may be related to antioxidant and anti-apoptotic mechanisms.https://www.mdpi.com/2076-2615/10/2/209melatoninembryooocytepig |
| spellingShingle | Ling Yang Qingkai Wang Maosheng Cui Qianjun Li Shuqin Mu Zimo Zhao Effect of Melatonin on the <i>In Vitro</i> Maturation of Porcine Oocytes, Development of Parthenogenetically Activated Embryos, and Expression of Genes Related to the Oocyte Developmental Capability Animals melatonin embryo oocyte pig |
| title | Effect of Melatonin on the <i>In Vitro</i> Maturation of Porcine Oocytes, Development of Parthenogenetically Activated Embryos, and Expression of Genes Related to the Oocyte Developmental Capability |
| title_full | Effect of Melatonin on the <i>In Vitro</i> Maturation of Porcine Oocytes, Development of Parthenogenetically Activated Embryos, and Expression of Genes Related to the Oocyte Developmental Capability |
| title_fullStr | Effect of Melatonin on the <i>In Vitro</i> Maturation of Porcine Oocytes, Development of Parthenogenetically Activated Embryos, and Expression of Genes Related to the Oocyte Developmental Capability |
| title_full_unstemmed | Effect of Melatonin on the <i>In Vitro</i> Maturation of Porcine Oocytes, Development of Parthenogenetically Activated Embryos, and Expression of Genes Related to the Oocyte Developmental Capability |
| title_short | Effect of Melatonin on the <i>In Vitro</i> Maturation of Porcine Oocytes, Development of Parthenogenetically Activated Embryos, and Expression of Genes Related to the Oocyte Developmental Capability |
| title_sort | effect of melatonin on the i in vitro i maturation of porcine oocytes development of parthenogenetically activated embryos and expression of genes related to the oocyte developmental capability |
| topic | melatonin embryo oocyte pig |
| url | https://www.mdpi.com/2076-2615/10/2/209 |
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