Effect of Dried Leaves of <i>Leucaena leucocephala</i> on Rumen Fermentation, Rumen Microbial Population, and Enteric Methane Production in Crossbred Heifers
The effects of dietary inclusion of dried <i>Leucaena</i> <i>leucocephala</i> leaves (DLL) on nutrient digestibility, fermentation parameters, microbial rumen population, and production of enteric methane (CH<sub>4</sub>) in crossbred heifers were evaluated. Four...
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MDPI AG
2020-02-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/10/2/300 |
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| author | María Denisse Montoya-Flores Isabel Cristina Molina-Botero Jacobo Arango José Luis Romano-Muñoz Francisco Javier Solorio-Sánchez Carlos Fernando Aguilar-Pérez Juan Carlos Ku-Vera |
| author_facet | María Denisse Montoya-Flores Isabel Cristina Molina-Botero Jacobo Arango José Luis Romano-Muñoz Francisco Javier Solorio-Sánchez Carlos Fernando Aguilar-Pérez Juan Carlos Ku-Vera |
| author_sort | María Denisse Montoya-Flores |
| collection | DOAJ |
| description | The effects of dietary inclusion of dried <i>Leucaena</i> <i>leucocephala</i> leaves (DLL) on nutrient digestibility, fermentation parameters, microbial rumen population, and production of enteric methane (CH<sub>4</sub>) in crossbred heifers were evaluated. Four heifers were used in a 4 × 4 Latin square design consisting of four periods and four levels of inclusion of DLL: 0%, 12%, 24%, and 36% of dry matter (DM) intake. Results showed that DM intake (DMI), organic matter intake, and gross energy intake (GEI) were similar (<i>p</i> > 0.05) among treatments. Apparent digestibility of organic matter, neutral detergent fiber, and energy decreased with increasing levels of DLL in the ration (<i>p</i> < 0.05). In contrast, digestible crude protein (CP) was higher (<i>p</i> < 0.05) in treatments with 12% and 24% DM of DLL. The inclusion of DLL did not affect (<i>p</i> > 0.05) rumen pH and total volatile fatty acids. Rumen microbial community was not affected (<i>p</i> > 0.05) by treatment. There was a linear reduction (<i>p</i> < 0.05) in CH<sub>4</sub> emissions as the levels of DLL in the ration were increased. Results of this study suggest that an inclusion of 12% DM of ration as DLL enhances digestible CP and reduces daily production of enteric CH<sub>4</sub> without adversely affecting DMI, rumen microbial population, and fermentation parameters. |
| first_indexed | 2024-12-22T23:31:54Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-12-22T23:31:54Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-0f063da06f1442a39447118a27d4a9ca2022-12-21T18:08:44ZengMDPI AGAnimals2076-26152020-02-0110230010.3390/ani10020300ani10020300Effect of Dried Leaves of <i>Leucaena leucocephala</i> on Rumen Fermentation, Rumen Microbial Population, and Enteric Methane Production in Crossbred HeifersMaría Denisse Montoya-Flores0Isabel Cristina Molina-Botero1Jacobo Arango2José Luis Romano-Muñoz3Francisco Javier Solorio-Sánchez4Carlos Fernando Aguilar-Pérez5Juan Carlos Ku-Vera6Faculty of Veterinary Medicine and Animal Science, Autonomous University of Yucatan, Mérida 97300, MexicoFaculty of Veterinary Medicine and Animal Science, Autonomous University of Yucatan, Mérida 97300, MexicoThe Alliance of Bioversity International and the International Center for Tropical Agriculture (CIAT), Km 17 Recta Cali-Palmira, A.A. 6713 Cali, ColombiaNational Center for Disciplinary Research in Physiology and Animal Breeding, National Institute for Forestry, Crops, and Livestock Research – Ministry of Agriculture and Rural Development, Ajuchitlán 76280, MexicoFaculty of Veterinary Medicine and Animal Science, Autonomous University of Yucatan, Mérida 97300, MexicoFaculty of Veterinary Medicine and Animal Science, Autonomous University of Yucatan, Mérida 97300, MexicoFaculty of Veterinary Medicine and Animal Science, Autonomous University of Yucatan, Mérida 97300, MexicoThe effects of dietary inclusion of dried <i>Leucaena</i> <i>leucocephala</i> leaves (DLL) on nutrient digestibility, fermentation parameters, microbial rumen population, and production of enteric methane (CH<sub>4</sub>) in crossbred heifers were evaluated. Four heifers were used in a 4 × 4 Latin square design consisting of four periods and four levels of inclusion of DLL: 0%, 12%, 24%, and 36% of dry matter (DM) intake. Results showed that DM intake (DMI), organic matter intake, and gross energy intake (GEI) were similar (<i>p</i> > 0.05) among treatments. Apparent digestibility of organic matter, neutral detergent fiber, and energy decreased with increasing levels of DLL in the ration (<i>p</i> < 0.05). In contrast, digestible crude protein (CP) was higher (<i>p</i> < 0.05) in treatments with 12% and 24% DM of DLL. The inclusion of DLL did not affect (<i>p</i> > 0.05) rumen pH and total volatile fatty acids. Rumen microbial community was not affected (<i>p</i> > 0.05) by treatment. There was a linear reduction (<i>p</i> < 0.05) in CH<sub>4</sub> emissions as the levels of DLL in the ration were increased. Results of this study suggest that an inclusion of 12% DM of ration as DLL enhances digestible CP and reduces daily production of enteric CH<sub>4</sub> without adversely affecting DMI, rumen microbial population, and fermentation parameters.https://www.mdpi.com/2076-2615/10/2/300<i>leucaena leucocephala</i>methanedigestibilityvolatile fatty acids |
| spellingShingle | María Denisse Montoya-Flores Isabel Cristina Molina-Botero Jacobo Arango José Luis Romano-Muñoz Francisco Javier Solorio-Sánchez Carlos Fernando Aguilar-Pérez Juan Carlos Ku-Vera Effect of Dried Leaves of <i>Leucaena leucocephala</i> on Rumen Fermentation, Rumen Microbial Population, and Enteric Methane Production in Crossbred Heifers Animals <i>leucaena leucocephala</i> methane digestibility volatile fatty acids |
| title | Effect of Dried Leaves of <i>Leucaena leucocephala</i> on Rumen Fermentation, Rumen Microbial Population, and Enteric Methane Production in Crossbred Heifers |
| title_full | Effect of Dried Leaves of <i>Leucaena leucocephala</i> on Rumen Fermentation, Rumen Microbial Population, and Enteric Methane Production in Crossbred Heifers |
| title_fullStr | Effect of Dried Leaves of <i>Leucaena leucocephala</i> on Rumen Fermentation, Rumen Microbial Population, and Enteric Methane Production in Crossbred Heifers |
| title_full_unstemmed | Effect of Dried Leaves of <i>Leucaena leucocephala</i> on Rumen Fermentation, Rumen Microbial Population, and Enteric Methane Production in Crossbred Heifers |
| title_short | Effect of Dried Leaves of <i>Leucaena leucocephala</i> on Rumen Fermentation, Rumen Microbial Population, and Enteric Methane Production in Crossbred Heifers |
| title_sort | effect of dried leaves of i leucaena leucocephala i on rumen fermentation rumen microbial population and enteric methane production in crossbred heifers |
| topic | <i>leucaena leucocephala</i> methane digestibility volatile fatty acids |
| url | https://www.mdpi.com/2076-2615/10/2/300 |
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