Expression of Transferrin Protein and Messenger RNA in Neural Cells from Mouse and Human Brain Tissue
Iron is an essential nutrient in the body. However, iron generates oxidative stress and hence needs to be bound to carrier proteins such as the glycoprotein transferrin (Tf) in body fluids. We previously reported that cerebrospinal fluid contains Tf glycan-isoforms that are derived from the brain, b...
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2022-06-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/12/7/594 |
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| author | Eriko Abe Takashi J. Fuwa Kyoka Hoshi Takashi Saito Takenobu Murakami Masakazu Miyajima Norihiro Ogawa Hiroyasu Akatsu Yoshio Hashizume Yasuhiro Hashimoto Takashi Honda |
| author_facet | Eriko Abe Takashi J. Fuwa Kyoka Hoshi Takashi Saito Takenobu Murakami Masakazu Miyajima Norihiro Ogawa Hiroyasu Akatsu Yoshio Hashizume Yasuhiro Hashimoto Takashi Honda |
| author_sort | Eriko Abe |
| collection | DOAJ |
| description | Iron is an essential nutrient in the body. However, iron generates oxidative stress and hence needs to be bound to carrier proteins such as the glycoprotein transferrin (Tf) in body fluids. We previously reported that cerebrospinal fluid contains Tf glycan-isoforms that are derived from the brain, but their origins at the cellular level in the brain have not yet been elucidated. In the present report, we described the localization of Tf protein and mRNA in mouse and human brain tissue. In situ hybridization of mouse brain tissue revealed that Tf mRNA is expressed by different cell types such as epithelial cells in the choroid plexus, oligodendrocyte-like cells in the medulla, and neurons in the cortex, hippocampus, and basal ganglia. In contrast, Tf protein was barely detected by immunohistochemistry in hippocampal and some cortical neurons, but it was detected in other types of cells such as oligodendrocyte-like cells and choroid plexus epithelial cells. The results showed that Tf mRNA is expressed by neural cells, while Tf protein is expressed in different brain regions, though at very low levels in hippocampal neurons. Low Tf level in the hippocampus may increases susceptibility to iron-induced oxidative stress, and account for neuron death in neurodegenerative diseases. |
| first_indexed | 2024-03-09T06:13:20Z |
| format | Article |
| id | doaj.art-0f0717c9ea1d4a13b3d98d1c67ce6dc0 |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-09T06:13:20Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-0f0717c9ea1d4a13b3d98d1c67ce6dc02023-12-03T11:55:48ZengMDPI AGMetabolites2218-19892022-06-0112759410.3390/metabo12070594Expression of Transferrin Protein and Messenger RNA in Neural Cells from Mouse and Human Brain TissueEriko Abe0Takashi J. Fuwa1Kyoka Hoshi2Takashi Saito3Takenobu Murakami4Masakazu Miyajima5Norihiro Ogawa6Hiroyasu Akatsu7Yoshio Hashizume8Yasuhiro Hashimoto9Takashi Honda10Department of Biochemistry, Fukushima Medical University, Fukushima 960-1295, JapanDepartment of Biochemistry, Fukushima Medical University, Fukushima 960-1295, JapanDepartment of Biochemistry, Fukushima Medical University, Fukushima 960-1295, JapanDepartment of Neurocognitive Science, Nagoya City University, Aichi 467-8601, JapanDivision of Neurology, Department of Brain and Neurosciences, Faculty of Medicine, Tottori University, Tottori 683-8504, JapanDepartment of Neurosurgery, Juntendo University, Tokyo 113-8421, JapanDepartment of Neuropathology, Fukushimura Hospital, Aichi 467-8601, JapanDepartment of Neuropathology, Fukushimura Hospital, Aichi 467-8601, JapanDepartment of Neuropathology, Fukushimura Hospital, Aichi 467-8601, JapanDepartment of Forensic Medicine, Fukushima Medical University, Fukushima 960-1295, JapanDepartment of Forensic Medicine, Fukushima Medical University, Fukushima 960-1295, JapanIron is an essential nutrient in the body. However, iron generates oxidative stress and hence needs to be bound to carrier proteins such as the glycoprotein transferrin (Tf) in body fluids. We previously reported that cerebrospinal fluid contains Tf glycan-isoforms that are derived from the brain, but their origins at the cellular level in the brain have not yet been elucidated. In the present report, we described the localization of Tf protein and mRNA in mouse and human brain tissue. In situ hybridization of mouse brain tissue revealed that Tf mRNA is expressed by different cell types such as epithelial cells in the choroid plexus, oligodendrocyte-like cells in the medulla, and neurons in the cortex, hippocampus, and basal ganglia. In contrast, Tf protein was barely detected by immunohistochemistry in hippocampal and some cortical neurons, but it was detected in other types of cells such as oligodendrocyte-like cells and choroid plexus epithelial cells. The results showed that Tf mRNA is expressed by neural cells, while Tf protein is expressed in different brain regions, though at very low levels in hippocampal neurons. Low Tf level in the hippocampus may increases susceptibility to iron-induced oxidative stress, and account for neuron death in neurodegenerative diseases.https://www.mdpi.com/2218-1989/12/7/594brainchoroid plexuscortexhippocampusmRNAneuron |
| spellingShingle | Eriko Abe Takashi J. Fuwa Kyoka Hoshi Takashi Saito Takenobu Murakami Masakazu Miyajima Norihiro Ogawa Hiroyasu Akatsu Yoshio Hashizume Yasuhiro Hashimoto Takashi Honda Expression of Transferrin Protein and Messenger RNA in Neural Cells from Mouse and Human Brain Tissue Metabolites brain choroid plexus cortex hippocampus mRNA neuron |
| title | Expression of Transferrin Protein and Messenger RNA in Neural Cells from Mouse and Human Brain Tissue |
| title_full | Expression of Transferrin Protein and Messenger RNA in Neural Cells from Mouse and Human Brain Tissue |
| title_fullStr | Expression of Transferrin Protein and Messenger RNA in Neural Cells from Mouse and Human Brain Tissue |
| title_full_unstemmed | Expression of Transferrin Protein and Messenger RNA in Neural Cells from Mouse and Human Brain Tissue |
| title_short | Expression of Transferrin Protein and Messenger RNA in Neural Cells from Mouse and Human Brain Tissue |
| title_sort | expression of transferrin protein and messenger rna in neural cells from mouse and human brain tissue |
| topic | brain choroid plexus cortex hippocampus mRNA neuron |
| url | https://www.mdpi.com/2218-1989/12/7/594 |
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