Functional Analysis of a Tannic-Acid-Inducible and Hypoviral-Regulated Small Heat-Shock Protein Hsp24 from the Chestnut Blight Fungus Cryphonectria parasitica
A small heat-shock protein gene, CpHsp24, of Cryphonectria parasitica was selected based on its expression pattern, which showed that it was tannic acid inducible and that its induction was severely hampered by a hypovirus. The predicted protein sequence of CpHsp24 consisted of a hallmark α-crystall...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2014-01-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-08-13-0225-R |
| _version_ | 1818647410186911744 |
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| author | Jin-Ho Baek Jin-Ah Park Jung-Mi Kim Jung-Mi Oh Seung-Moon Park Dae-Hyuk Kim |
| author_facet | Jin-Ho Baek Jin-Ah Park Jung-Mi Kim Jung-Mi Oh Seung-Moon Park Dae-Hyuk Kim |
| author_sort | Jin-Ho Baek |
| collection | DOAJ |
| description | A small heat-shock protein gene, CpHsp24, of Cryphonectria parasitica was selected based on its expression pattern, which showed that it was tannic acid inducible and that its induction was severely hampered by a hypovirus. The predicted protein sequence of CpHsp24 consisted of a hallmark α-crystalline domain flanked by a variable N-terminal and a short C-terminal region. Disruption of CpHsp24 resulted in a slow growth rate under standard growth conditions. The CpHsp24-null mutant showed enhanced sensitivity to heat shock, which was consistent with Northern and Western analyses displaying the heat-shock induction of the CpHsp24 gene and protein, respectively. Virulence tests on the excised bark revealed a severe decrease in the necrotic area of the CpHsp24-null mutant. When the hypovirus was transferred, virus-containing CpHsp24-null progeny displayed severely retarded growth patterns with hypovirulent characteristics of reduced pigmentation and sporulation. Because the tannic-acid-inducible and hypoviral-suppressible expression and the severely impaired virulence are also characteristics of the laccase3 gene (lac3), lac3 expression in the CpHsp24-null mutant was also examined. The resulting lac3 induction was severely affected in the CpHsp24-null mutant, suggesting that CpHsp24 is important for lac3 induction and that CpHsp24 may act as a molecular chaperone for the lac3 protein. |
| first_indexed | 2024-12-17T01:02:05Z |
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| id | doaj.art-0f0777493cea4eeb8d9e64e8d84b9858 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-17T01:02:05Z |
| publishDate | 2014-01-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-0f0777493cea4eeb8d9e64e8d84b98582022-12-21T22:09:24ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062014-01-01271566510.1094/MPMI-08-13-0225-RFunctional Analysis of a Tannic-Acid-Inducible and Hypoviral-Regulated Small Heat-Shock Protein Hsp24 from the Chestnut Blight Fungus Cryphonectria parasiticaJin-Ho BaekJin-Ah ParkJung-Mi KimJung-Mi OhSeung-Moon ParkDae-Hyuk KimA small heat-shock protein gene, CpHsp24, of Cryphonectria parasitica was selected based on its expression pattern, which showed that it was tannic acid inducible and that its induction was severely hampered by a hypovirus. The predicted protein sequence of CpHsp24 consisted of a hallmark α-crystalline domain flanked by a variable N-terminal and a short C-terminal region. Disruption of CpHsp24 resulted in a slow growth rate under standard growth conditions. The CpHsp24-null mutant showed enhanced sensitivity to heat shock, which was consistent with Northern and Western analyses displaying the heat-shock induction of the CpHsp24 gene and protein, respectively. Virulence tests on the excised bark revealed a severe decrease in the necrotic area of the CpHsp24-null mutant. When the hypovirus was transferred, virus-containing CpHsp24-null progeny displayed severely retarded growth patterns with hypovirulent characteristics of reduced pigmentation and sporulation. Because the tannic-acid-inducible and hypoviral-suppressible expression and the severely impaired virulence are also characteristics of the laccase3 gene (lac3), lac3 expression in the CpHsp24-null mutant was also examined. The resulting lac3 induction was severely affected in the CpHsp24-null mutant, suggesting that CpHsp24 is important for lac3 induction and that CpHsp24 may act as a molecular chaperone for the lac3 protein.https://apsjournals.apsnet.org/doi/10.1094/MPMI-08-13-0225-R |
| spellingShingle | Jin-Ho Baek Jin-Ah Park Jung-Mi Kim Jung-Mi Oh Seung-Moon Park Dae-Hyuk Kim Functional Analysis of a Tannic-Acid-Inducible and Hypoviral-Regulated Small Heat-Shock Protein Hsp24 from the Chestnut Blight Fungus Cryphonectria parasitica Molecular Plant-Microbe Interactions |
| title | Functional Analysis of a Tannic-Acid-Inducible and Hypoviral-Regulated Small Heat-Shock Protein Hsp24 from the Chestnut Blight Fungus Cryphonectria parasitica |
| title_full | Functional Analysis of a Tannic-Acid-Inducible and Hypoviral-Regulated Small Heat-Shock Protein Hsp24 from the Chestnut Blight Fungus Cryphonectria parasitica |
| title_fullStr | Functional Analysis of a Tannic-Acid-Inducible and Hypoviral-Regulated Small Heat-Shock Protein Hsp24 from the Chestnut Blight Fungus Cryphonectria parasitica |
| title_full_unstemmed | Functional Analysis of a Tannic-Acid-Inducible and Hypoviral-Regulated Small Heat-Shock Protein Hsp24 from the Chestnut Blight Fungus Cryphonectria parasitica |
| title_short | Functional Analysis of a Tannic-Acid-Inducible and Hypoviral-Regulated Small Heat-Shock Protein Hsp24 from the Chestnut Blight Fungus Cryphonectria parasitica |
| title_sort | functional analysis of a tannic acid inducible and hypoviral regulated small heat shock protein hsp24 from the chestnut blight fungus cryphonectria parasitica |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-08-13-0225-R |
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