Evidence of Illegitimate Recombination Between Two Pasteurellaceae Plasmids Resulting in a Novel Multi-Resistance Replicon, pM3362MDR, in Actinobacillus pleuropneumoniae
Evidence of plasmids carrying the tetracycline resistance gene, tet(B), was found in the previously reported whole genome sequences of 14 United Kingdom, and 4 Brazilian, isolates of Actinobacillus pleuropneumoniae. Isolation and sequencing of selected plasmids, combined with comparative sequence an...
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| Format: | Article |
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Frontiers Media S.A.
2018-10-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2018.02489/full |
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| author | Yinghui Li Yinghui Li Giarlã Cunha da Silva Yanwen Li Ciro C. Rossi Roberto Fernandez Crespo Susanna M. Williamson Paul R. Langford Denise Mara Soares Bazzolli Janine T. Bossé |
| author_facet | Yinghui Li Yinghui Li Giarlã Cunha da Silva Yanwen Li Ciro C. Rossi Roberto Fernandez Crespo Susanna M. Williamson Paul R. Langford Denise Mara Soares Bazzolli Janine T. Bossé |
| author_sort | Yinghui Li |
| collection | DOAJ |
| description | Evidence of plasmids carrying the tetracycline resistance gene, tet(B), was found in the previously reported whole genome sequences of 14 United Kingdom, and 4 Brazilian, isolates of Actinobacillus pleuropneumoniae. Isolation and sequencing of selected plasmids, combined with comparative sequence analysis, indicated that the four Brazilian isolates all harbor plasmids that are nearly identical to pB1001, a plasmid previously found in Pasteurella multocida isolates from Spain. Of the United Kingdom isolates, 13/14 harbor plasmids that are (almost) identical to pTetHS016 from Haemophilus parasuis. The remaining United Kingdom isolate, MIDG3362, harbors a 12666 bp plasmid that shares extensive regions of similarity with pOV from P. multocida (which carries blaROB-1, sul2, and strAB genes), as well as with pTetHS016. The newly identified multi-resistance plasmid, pM3362MDR, appears to have arisen through illegitimate recombination of pTetHS016 into the stop codon of the truncated strB gene in a pOV-like plasmid. All of the tet(B)-carrying plasmids studied were capable of replicating in Escherichia coli, and predicted origins of replication were identified. A putative origin of transfer (oriT) sequence with similar secondary structure and a nic-site almost identical to that of RP4 was also identified in these plasmids, however, attempts to mobilize them from an RP4-encoding E. coli donor strain were not successful, indicating that specific conjugation machinery may be required. |
| first_indexed | 2024-12-12T19:45:38Z |
| format | Article |
| id | doaj.art-0f090ca1456c4e33b46d8e7366e055a2 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-12T19:45:38Z |
| publishDate | 2018-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-0f090ca1456c4e33b46d8e7366e055a22022-12-22T00:14:05ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-10-01910.3389/fmicb.2018.02489410580Evidence of Illegitimate Recombination Between Two Pasteurellaceae Plasmids Resulting in a Novel Multi-Resistance Replicon, pM3362MDR, in Actinobacillus pleuropneumoniaeYinghui Li0Yinghui Li1Giarlã Cunha da Silva2Yanwen Li3Ciro C. Rossi4Roberto Fernandez Crespo5Susanna M. Williamson6Paul R. Langford7Denise Mara Soares Bazzolli8Janine T. Bossé9Section of Paediatrics, Department of Medicine, Imperial College London, London, United KingdomShenzhen Center for Disease Control and Prevention, Shenzhen, ChinaLaboratório de Genética Molecular de Bactérias, Departamento de Microbiologia, Instituto de Biotecnologia Aplicada à Agropecuária, Universidade Federal de Viçosa, Viçosa, BrazilSection of Paediatrics, Department of Medicine, Imperial College London, London, United KingdomLaboratório de Genética Molecular de Bactérias, Departamento de Microbiologia, Instituto de Biotecnologia Aplicada à Agropecuária, Universidade Federal de Viçosa, Viçosa, BrazilSection of Paediatrics, Department of Medicine, Imperial College London, London, United KingdomAnimal and Plant Health Agency, Addlestone, United KingdomSection of Paediatrics, Department of Medicine, Imperial College London, London, United KingdomLaboratório de Genética Molecular de Bactérias, Departamento de Microbiologia, Instituto de Biotecnologia Aplicada à Agropecuária, Universidade Federal de Viçosa, Viçosa, BrazilSection of Paediatrics, Department of Medicine, Imperial College London, London, United KingdomEvidence of plasmids carrying the tetracycline resistance gene, tet(B), was found in the previously reported whole genome sequences of 14 United Kingdom, and 4 Brazilian, isolates of Actinobacillus pleuropneumoniae. Isolation and sequencing of selected plasmids, combined with comparative sequence analysis, indicated that the four Brazilian isolates all harbor plasmids that are nearly identical to pB1001, a plasmid previously found in Pasteurella multocida isolates from Spain. Of the United Kingdom isolates, 13/14 harbor plasmids that are (almost) identical to pTetHS016 from Haemophilus parasuis. The remaining United Kingdom isolate, MIDG3362, harbors a 12666 bp plasmid that shares extensive regions of similarity with pOV from P. multocida (which carries blaROB-1, sul2, and strAB genes), as well as with pTetHS016. The newly identified multi-resistance plasmid, pM3362MDR, appears to have arisen through illegitimate recombination of pTetHS016 into the stop codon of the truncated strB gene in a pOV-like plasmid. All of the tet(B)-carrying plasmids studied were capable of replicating in Escherichia coli, and predicted origins of replication were identified. A putative origin of transfer (oriT) sequence with similar secondary structure and a nic-site almost identical to that of RP4 was also identified in these plasmids, however, attempts to mobilize them from an RP4-encoding E. coli donor strain were not successful, indicating that specific conjugation machinery may be required.https://www.frontiersin.org/article/10.3389/fmicb.2018.02489/fullplasmidsantimicrobial resistancetetracyclinerespiratory tractPasteurellaceae |
| spellingShingle | Yinghui Li Yinghui Li Giarlã Cunha da Silva Yanwen Li Ciro C. Rossi Roberto Fernandez Crespo Susanna M. Williamson Paul R. Langford Denise Mara Soares Bazzolli Janine T. Bossé Evidence of Illegitimate Recombination Between Two Pasteurellaceae Plasmids Resulting in a Novel Multi-Resistance Replicon, pM3362MDR, in Actinobacillus pleuropneumoniae Frontiers in Microbiology plasmids antimicrobial resistance tetracycline respiratory tract Pasteurellaceae |
| title | Evidence of Illegitimate Recombination Between Two Pasteurellaceae Plasmids Resulting in a Novel Multi-Resistance Replicon, pM3362MDR, in Actinobacillus pleuropneumoniae |
| title_full | Evidence of Illegitimate Recombination Between Two Pasteurellaceae Plasmids Resulting in a Novel Multi-Resistance Replicon, pM3362MDR, in Actinobacillus pleuropneumoniae |
| title_fullStr | Evidence of Illegitimate Recombination Between Two Pasteurellaceae Plasmids Resulting in a Novel Multi-Resistance Replicon, pM3362MDR, in Actinobacillus pleuropneumoniae |
| title_full_unstemmed | Evidence of Illegitimate Recombination Between Two Pasteurellaceae Plasmids Resulting in a Novel Multi-Resistance Replicon, pM3362MDR, in Actinobacillus pleuropneumoniae |
| title_short | Evidence of Illegitimate Recombination Between Two Pasteurellaceae Plasmids Resulting in a Novel Multi-Resistance Replicon, pM3362MDR, in Actinobacillus pleuropneumoniae |
| title_sort | evidence of illegitimate recombination between two pasteurellaceae plasmids resulting in a novel multi resistance replicon pm3362mdr in actinobacillus pleuropneumoniae |
| topic | plasmids antimicrobial resistance tetracycline respiratory tract Pasteurellaceae |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2018.02489/full |
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