Characterization of the Plasma Lipidome in Dairy Cattle Transitioning from Gestation to Lactation: Identifying Novel Biomarkers of Metabolic Impairment
The discovery of novel biomarkers for peripartal diseases in dairy cows can improve our understanding of normal and dysfunctional metabolism, and lead to nutritional interventions that improve health and milk production. Our objectives were to characterize the plasma lipidome and identify metabolite...
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MDPI AG
2021-04-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/11/5/290 |
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| author | Jorge Eduardo Rico Sina Saed Samii Yu Zang Pragney Deme Norman J. Haughey Ester Grilli Joseph W. McFadden |
| author_facet | Jorge Eduardo Rico Sina Saed Samii Yu Zang Pragney Deme Norman J. Haughey Ester Grilli Joseph W. McFadden |
| author_sort | Jorge Eduardo Rico |
| collection | DOAJ |
| description | The discovery of novel biomarkers for peripartal diseases in dairy cows can improve our understanding of normal and dysfunctional metabolism, and lead to nutritional interventions that improve health and milk production. Our objectives were to characterize the plasma lipidome and identify metabolites associated with common markers of metabolic disease in peripartal dairy cattle. Multiparous Holstein cows (<i>n</i> = 27) were enrolled 30 d prior to expected parturition. Blood and liver samples were routinely collected through to d 14 postpartum. Untargeted lipidomics was performed using quadrupole time-of-flight mass spectrometry. Based on postpartum measures, cows were categorized into low or high total fatty acid area under the curve (total FAAUC; d 1–14 postpartum; 4915 ± 1369 vs. 12,501 ± 2761 (μmol/L × 14 d); <i>n</i> = 18), β-hydroxybutyrate AUC (BHBAAUC; d 1–14 postpartum; 4583 ± 459 vs. 7901 ± 1206 (μmol/L × 14 d); <i>n</i> = 18), or liver lipid content (d 5 and 14 postpartum; 5 ± 1 vs. 12 ± 2% of wet weight; <i>n</i> = 18). Cows displayed decreases in plasma triacylglycerols and monoalkyl-diacylglycerols, and the majority of phospholipids reached a nadir at parturition. Phosphatidylcholines (PC) 32:3, 35:5, and 37:5 were specific for high total FAAUC, PC 31:3, 32:3, 35:5, and 37:5 were specific for high BHBAAUC, and PC 31:2, 31:3, and 32:3 were specific for high liver lipid content. PC 32:3 was specific for elevated total FA, BHBA, and liver lipid content. Lipidomics revealed a dynamic peripartal lipidome remodeling, and lipid markers associated with elevated total FA, BHBA, and liver lipid content. The effectiveness of nutrition to impact these lipid biomarkers for preventing excess lipolysis and fatty liver warrants evaluation. |
| first_indexed | 2024-03-10T11:46:54Z |
| format | Article |
| id | doaj.art-0f098e6cf2ef449083f72fb954542af5 |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-10T11:46:54Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-0f098e6cf2ef449083f72fb954542af52023-11-21T18:01:07ZengMDPI AGMetabolites2218-19892021-04-0111529010.3390/metabo11050290Characterization of the Plasma Lipidome in Dairy Cattle Transitioning from Gestation to Lactation: Identifying Novel Biomarkers of Metabolic ImpairmentJorge Eduardo Rico0Sina Saed Samii1Yu Zang2Pragney Deme3Norman J. Haughey4Ester Grilli5Joseph W. McFadden6Department of Animal Science, Cornell University, Ithaca, NY 14853, USADivision of Animal and Nutritional Sciences, West Virginia University, Morgantown, WV 26505, USADivision of Animal and Nutritional Sciences, West Virginia University, Morgantown, WV 26505, USADepartment of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USADepartment of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USADepartment of Veterinary Medical Sciences, University of Bologna, 40064 Bologna, ItalyDepartment of Animal Science, Cornell University, Ithaca, NY 14853, USAThe discovery of novel biomarkers for peripartal diseases in dairy cows can improve our understanding of normal and dysfunctional metabolism, and lead to nutritional interventions that improve health and milk production. Our objectives were to characterize the plasma lipidome and identify metabolites associated with common markers of metabolic disease in peripartal dairy cattle. Multiparous Holstein cows (<i>n</i> = 27) were enrolled 30 d prior to expected parturition. Blood and liver samples were routinely collected through to d 14 postpartum. Untargeted lipidomics was performed using quadrupole time-of-flight mass spectrometry. Based on postpartum measures, cows were categorized into low or high total fatty acid area under the curve (total FAAUC; d 1–14 postpartum; 4915 ± 1369 vs. 12,501 ± 2761 (μmol/L × 14 d); <i>n</i> = 18), β-hydroxybutyrate AUC (BHBAAUC; d 1–14 postpartum; 4583 ± 459 vs. 7901 ± 1206 (μmol/L × 14 d); <i>n</i> = 18), or liver lipid content (d 5 and 14 postpartum; 5 ± 1 vs. 12 ± 2% of wet weight; <i>n</i> = 18). Cows displayed decreases in plasma triacylglycerols and monoalkyl-diacylglycerols, and the majority of phospholipids reached a nadir at parturition. Phosphatidylcholines (PC) 32:3, 35:5, and 37:5 were specific for high total FAAUC, PC 31:3, 32:3, 35:5, and 37:5 were specific for high BHBAAUC, and PC 31:2, 31:3, and 32:3 were specific for high liver lipid content. PC 32:3 was specific for elevated total FA, BHBA, and liver lipid content. Lipidomics revealed a dynamic peripartal lipidome remodeling, and lipid markers associated with elevated total FA, BHBA, and liver lipid content. The effectiveness of nutrition to impact these lipid biomarkers for preventing excess lipolysis and fatty liver warrants evaluation.https://www.mdpi.com/2218-1989/11/5/290biomarkercow healthperipartumlipidomics |
| spellingShingle | Jorge Eduardo Rico Sina Saed Samii Yu Zang Pragney Deme Norman J. Haughey Ester Grilli Joseph W. McFadden Characterization of the Plasma Lipidome in Dairy Cattle Transitioning from Gestation to Lactation: Identifying Novel Biomarkers of Metabolic Impairment Metabolites biomarker cow health peripartum lipidomics |
| title | Characterization of the Plasma Lipidome in Dairy Cattle Transitioning from Gestation to Lactation: Identifying Novel Biomarkers of Metabolic Impairment |
| title_full | Characterization of the Plasma Lipidome in Dairy Cattle Transitioning from Gestation to Lactation: Identifying Novel Biomarkers of Metabolic Impairment |
| title_fullStr | Characterization of the Plasma Lipidome in Dairy Cattle Transitioning from Gestation to Lactation: Identifying Novel Biomarkers of Metabolic Impairment |
| title_full_unstemmed | Characterization of the Plasma Lipidome in Dairy Cattle Transitioning from Gestation to Lactation: Identifying Novel Biomarkers of Metabolic Impairment |
| title_short | Characterization of the Plasma Lipidome in Dairy Cattle Transitioning from Gestation to Lactation: Identifying Novel Biomarkers of Metabolic Impairment |
| title_sort | characterization of the plasma lipidome in dairy cattle transitioning from gestation to lactation identifying novel biomarkers of metabolic impairment |
| topic | biomarker cow health peripartum lipidomics |
| url | https://www.mdpi.com/2218-1989/11/5/290 |
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