Melatonin Promotes In Vitro Maturation of Vitrified-Warmed Mouse Germinal Vesicle Oocytes, Potentially by Reducing Oxidative Stress through the Nrf2 Pathway
Previously it was reported that melatonin could mitigate oxidative stress caused by oocyte cryopreservation; however, the underlying molecular mechanisms which cause this remain unclear. The objective was to explore whether melatonin could reduce oxidative stress during in vitro maturation of vitrif...
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MDPI AG
2021-08-01
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| Online Access: | https://www.mdpi.com/2076-2615/11/8/2324 |
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| author | Shichao Guo Jinyu Yang Jianpeng Qin Izhar Hyder Qazi Bo Pan Shengqin Zang Tianyi Lv Shoulong Deng Yi Fang Guangbin Zhou |
| author_facet | Shichao Guo Jinyu Yang Jianpeng Qin Izhar Hyder Qazi Bo Pan Shengqin Zang Tianyi Lv Shoulong Deng Yi Fang Guangbin Zhou |
| author_sort | Shichao Guo |
| collection | DOAJ |
| description | Previously it was reported that melatonin could mitigate oxidative stress caused by oocyte cryopreservation; however, the underlying molecular mechanisms which cause this remain unclear. The objective was to explore whether melatonin could reduce oxidative stress during in vitro maturation of vitrified-warmed mouse germinal vesicle (GV) oocytes through the Nrf2 signaling pathway or its receptors. During in vitro maturation of vitrified-warmed mouse GV oocytes, there were decreases (<i>p</i> < 0.05) in the development rates of metaphase I (MI) oocytes and metaphase II (MII) and spindle morphology grades; increases (<i>p</i> < 0.05) in the reactive oxygen species (ROS) levels; and decreases (<i>p</i> < 0.05) in expressions of Nrf2 signaling pathway-related genes (<i>Nrf2</i>, <i>SOD1</i>) and proteins (Nrf2, HO-1). However, adding 10<sup>−7</sup> mol/L melatonin to both the warming solution and maturation solutions improved (<i>p</i> < 0.05) these indicators. When the Nrf2 protein was specifically inhibited by Brusatol, melatonin did not increase development rates, spindle morphology grades, genes, or protein expressions, nor did it reduce vitrification-induced intracellular oxidative stress in GV oocytes during in vitro maturation. In addition, when melatonin receptors were inhibited by luzindole, the ability of melatonin to scavenge intracellular ROS was decreased, and the expressions of genes (<i>Nrf2</i>, <i>SOD1</i>) and proteins (Nrf2, HO-1) were not restored to control levels. Therefore, we concluded that 10<sup>−7</sup> mol/L melatonin acted on the Nrf2 signaling pathway through its receptors to regulate the expression of genes (<i>Nrf2</i>, <i>SOD1</i>) and proteins (Nrf2, HO-1), and mitigate intracellular oxidative stress, thereby enhancing in vitro development of vitrified-warmed mouse GV oocytes. |
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| language | English |
| last_indexed | 2024-03-10T09:03:58Z |
| publishDate | 2021-08-01 |
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| spelling | doaj.art-0f0caa57491e4756af81c55f6ba692fa2023-11-22T06:30:30ZengMDPI AGAnimals2076-26152021-08-01118232410.3390/ani11082324Melatonin Promotes In Vitro Maturation of Vitrified-Warmed Mouse Germinal Vesicle Oocytes, Potentially by Reducing Oxidative Stress through the Nrf2 PathwayShichao Guo0Jinyu Yang1Jianpeng Qin2Izhar Hyder Qazi3Bo Pan4Shengqin Zang5Tianyi Lv6Shoulong Deng7Yi Fang8Guangbin Zhou9Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaDepartment of Veterinary Anatomy & Histology, Shaheed Benazir Bhutto University of Veterinary and Animal Sciences, Sindh, Sakrand 67210, PakistanFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaInstitute of Laboratory Animal Sciences, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College, Beijing 100021, ChinaJilin Provincial Key Laboratory of Grassland Farming, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, ChinaFarm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, ChinaPreviously it was reported that melatonin could mitigate oxidative stress caused by oocyte cryopreservation; however, the underlying molecular mechanisms which cause this remain unclear. The objective was to explore whether melatonin could reduce oxidative stress during in vitro maturation of vitrified-warmed mouse germinal vesicle (GV) oocytes through the Nrf2 signaling pathway or its receptors. During in vitro maturation of vitrified-warmed mouse GV oocytes, there were decreases (<i>p</i> < 0.05) in the development rates of metaphase I (MI) oocytes and metaphase II (MII) and spindle morphology grades; increases (<i>p</i> < 0.05) in the reactive oxygen species (ROS) levels; and decreases (<i>p</i> < 0.05) in expressions of Nrf2 signaling pathway-related genes (<i>Nrf2</i>, <i>SOD1</i>) and proteins (Nrf2, HO-1). However, adding 10<sup>−7</sup> mol/L melatonin to both the warming solution and maturation solutions improved (<i>p</i> < 0.05) these indicators. When the Nrf2 protein was specifically inhibited by Brusatol, melatonin did not increase development rates, spindle morphology grades, genes, or protein expressions, nor did it reduce vitrification-induced intracellular oxidative stress in GV oocytes during in vitro maturation. In addition, when melatonin receptors were inhibited by luzindole, the ability of melatonin to scavenge intracellular ROS was decreased, and the expressions of genes (<i>Nrf2</i>, <i>SOD1</i>) and proteins (Nrf2, HO-1) were not restored to control levels. Therefore, we concluded that 10<sup>−7</sup> mol/L melatonin acted on the Nrf2 signaling pathway through its receptors to regulate the expression of genes (<i>Nrf2</i>, <i>SOD1</i>) and proteins (Nrf2, HO-1), and mitigate intracellular oxidative stress, thereby enhancing in vitro development of vitrified-warmed mouse GV oocytes.https://www.mdpi.com/2076-2615/11/8/2324vitrificationreceptorsantioxidantsreactive oxygen speciesNrf2 |
| spellingShingle | Shichao Guo Jinyu Yang Jianpeng Qin Izhar Hyder Qazi Bo Pan Shengqin Zang Tianyi Lv Shoulong Deng Yi Fang Guangbin Zhou Melatonin Promotes In Vitro Maturation of Vitrified-Warmed Mouse Germinal Vesicle Oocytes, Potentially by Reducing Oxidative Stress through the Nrf2 Pathway Animals vitrification receptors antioxidants reactive oxygen species Nrf2 |
| title | Melatonin Promotes In Vitro Maturation of Vitrified-Warmed Mouse Germinal Vesicle Oocytes, Potentially by Reducing Oxidative Stress through the Nrf2 Pathway |
| title_full | Melatonin Promotes In Vitro Maturation of Vitrified-Warmed Mouse Germinal Vesicle Oocytes, Potentially by Reducing Oxidative Stress through the Nrf2 Pathway |
| title_fullStr | Melatonin Promotes In Vitro Maturation of Vitrified-Warmed Mouse Germinal Vesicle Oocytes, Potentially by Reducing Oxidative Stress through the Nrf2 Pathway |
| title_full_unstemmed | Melatonin Promotes In Vitro Maturation of Vitrified-Warmed Mouse Germinal Vesicle Oocytes, Potentially by Reducing Oxidative Stress through the Nrf2 Pathway |
| title_short | Melatonin Promotes In Vitro Maturation of Vitrified-Warmed Mouse Germinal Vesicle Oocytes, Potentially by Reducing Oxidative Stress through the Nrf2 Pathway |
| title_sort | melatonin promotes in vitro maturation of vitrified warmed mouse germinal vesicle oocytes potentially by reducing oxidative stress through the nrf2 pathway |
| topic | vitrification receptors antioxidants reactive oxygen species Nrf2 |
| url | https://www.mdpi.com/2076-2615/11/8/2324 |
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