Functional Characterization and Genomic Analysis of the Chlorantraniliprole-Degrading Strain <i>Pseudomonas</i> Sp. GW13
Chlorantraniliprole (CAP) is a widely used insecticide in many areas due to its excellent insecticidal ability and mammalian safety, however, the removal of CAP has not been extensively studied. In this study, a bacterial strain GW13, which is capable of co-metabolizing CAP, was isolated from a vege...
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MDPI AG
2019-11-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/6/4/106 |
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| author | Wa Gao Dongyang Li Hong You |
| author_facet | Wa Gao Dongyang Li Hong You |
| author_sort | Wa Gao |
| collection | DOAJ |
| description | Chlorantraniliprole (CAP) is a widely used insecticide in many areas due to its excellent insecticidal ability and mammalian safety, however, the removal of CAP has not been extensively studied. In this study, a bacterial strain GW13, which is capable of co-metabolizing CAP, was isolated from a vegetable field soil. The strain was identified as <i>Pseudomonas</i> sp. based on its physico-biochemical characteristics and 16S rRNA gene analysis. The bacterial strain GW13 could degrade CAP through co-metabolism, and glucose was the best additional carbon resource. In the presence of 1.0 g/L glucose, GW13 could co-metabolize over 80% of 200 mg/L CAP in 24 h. The degradation rate increased after 6 h and slowed again after 10 h. The GW13 genome analysis revealed many genes associated with metabolism, showing the degradation mechanism of GW13 from the genomic perspective. The EAWAG-BBD (Swiss Federal Institute of Aquatic Science and Technology Biocatalysis/Biodegradation Database) prediction results showed that the main pathway for CAP degradation is amide hydrolysis, which is consistent with many genes associated with amidase in the GW13 genome. This study may facilitate research on CAP biodegradation mechanisms in the environment. |
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| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-12T07:13:46Z |
| publishDate | 2019-11-01 |
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| spelling | doaj.art-0f0ccf3abf6c4febae71464ebf81f9922023-09-02T22:56:07ZengMDPI AGBioengineering2306-53542019-11-016410610.3390/bioengineering6040106bioengineering6040106Functional Characterization and Genomic Analysis of the Chlorantraniliprole-Degrading Strain <i>Pseudomonas</i> Sp. GW13Wa Gao0Dongyang Li1Hong You2Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Key Laboratory of Fermentation Engineering (Ministry of Education), School of Food and Biological Engineering, Hubei University of Technology, Wuhan 430068, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang, Henan 455000, ChinaHubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaChlorantraniliprole (CAP) is a widely used insecticide in many areas due to its excellent insecticidal ability and mammalian safety, however, the removal of CAP has not been extensively studied. In this study, a bacterial strain GW13, which is capable of co-metabolizing CAP, was isolated from a vegetable field soil. The strain was identified as <i>Pseudomonas</i> sp. based on its physico-biochemical characteristics and 16S rRNA gene analysis. The bacterial strain GW13 could degrade CAP through co-metabolism, and glucose was the best additional carbon resource. In the presence of 1.0 g/L glucose, GW13 could co-metabolize over 80% of 200 mg/L CAP in 24 h. The degradation rate increased after 6 h and slowed again after 10 h. The GW13 genome analysis revealed many genes associated with metabolism, showing the degradation mechanism of GW13 from the genomic perspective. The EAWAG-BBD (Swiss Federal Institute of Aquatic Science and Technology Biocatalysis/Biodegradation Database) prediction results showed that the main pathway for CAP degradation is amide hydrolysis, which is consistent with many genes associated with amidase in the GW13 genome. This study may facilitate research on CAP biodegradation mechanisms in the environment.https://www.mdpi.com/2306-5354/6/4/106chlorantraniliproleco-metabolismpseudomonasdraft genome |
| spellingShingle | Wa Gao Dongyang Li Hong You Functional Characterization and Genomic Analysis of the Chlorantraniliprole-Degrading Strain <i>Pseudomonas</i> Sp. GW13 Bioengineering chlorantraniliprole co-metabolism pseudomonas draft genome |
| title | Functional Characterization and Genomic Analysis of the Chlorantraniliprole-Degrading Strain <i>Pseudomonas</i> Sp. GW13 |
| title_full | Functional Characterization and Genomic Analysis of the Chlorantraniliprole-Degrading Strain <i>Pseudomonas</i> Sp. GW13 |
| title_fullStr | Functional Characterization and Genomic Analysis of the Chlorantraniliprole-Degrading Strain <i>Pseudomonas</i> Sp. GW13 |
| title_full_unstemmed | Functional Characterization and Genomic Analysis of the Chlorantraniliprole-Degrading Strain <i>Pseudomonas</i> Sp. GW13 |
| title_short | Functional Characterization and Genomic Analysis of the Chlorantraniliprole-Degrading Strain <i>Pseudomonas</i> Sp. GW13 |
| title_sort | functional characterization and genomic analysis of the chlorantraniliprole degrading strain i pseudomonas i sp gw13 |
| topic | chlorantraniliprole co-metabolism pseudomonas draft genome |
| url | https://www.mdpi.com/2306-5354/6/4/106 |
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