Evaluation of 16S rDNA Heart Tissue PCR as a Complement to Blood Cultures for the Routine Etiological Diagnosis of Infective Endocarditis
Identification of the causative pathogen is required to optimize the effective therapy in infective endocarditis (IE). The aim of this study was to assess a 16S rDNA PCR to identify bacteria from heart valve tissues and to evaluate its usefulness as a complement to blood and removed valves cultures....
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-07-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/11/8/1372 |
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| author | Raquel Rodríguez-García María Ángeles Rodríguez-Esteban Jonathan Fernández-Suárez Ana Morilla Enrique García-Carús Mauricio Telenti Carlos Morales Guillermo Muñiz Albaiceta Javier Fernández |
| author_facet | Raquel Rodríguez-García María Ángeles Rodríguez-Esteban Jonathan Fernández-Suárez Ana Morilla Enrique García-Carús Mauricio Telenti Carlos Morales Guillermo Muñiz Albaiceta Javier Fernández |
| author_sort | Raquel Rodríguez-García |
| collection | DOAJ |
| description | Identification of the causative pathogen is required to optimize the effective therapy in infective endocarditis (IE). The aim of this study was to assess a 16S rDNA PCR to identify bacteria from heart valve tissues and to evaluate its usefulness as a complement to blood and removed valves cultures. A total of 266 patients diagnosed with IE from January 2015 to December 2019 were evaluated. Results between 16S rDNA PCR from heart valve tissues were compared with microbiological cultures. Blood cultures were positive in 83.5% of patients diagnosed with IE, while 39.6% and 71.8% of the evaluated heart valve samples were positive by culture and 16S rDNA PCR, respectively. For 32 (12%) patients, 16S rDNA tissue PCR provided valuable information supporting the results of blood cultures in the case of bacteria characteristic from the skin microbiota. Additionally, a microorganism was identified by using 16S rDNA PCR in 36% of blood culture-negative cases. The present study reveals that molecular diagnosis using 16S rDNA tissue PCR provides complementary information for the diagnosis of IE, and it should be recommended in surgical endocarditis, especially when blood cultures are negative. |
| first_indexed | 2024-03-10T08:53:15Z |
| format | Article |
| id | doaj.art-0f0cdb15d4774913bade7835ffd575a1 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-10T08:53:15Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-0f0cdb15d4774913bade7835ffd575a12023-11-22T07:19:33ZengMDPI AGDiagnostics2075-44182021-07-01118137210.3390/diagnostics11081372Evaluation of 16S rDNA Heart Tissue PCR as a Complement to Blood Cultures for the Routine Etiological Diagnosis of Infective EndocarditisRaquel Rodríguez-García0María Ángeles Rodríguez-Esteban1Jonathan Fernández-Suárez2Ana Morilla3Enrique García-Carús4Mauricio Telenti5Carlos Morales6Guillermo Muñiz Albaiceta7Javier Fernández8Unidad de Cuidados Intensivos Cardiológicos, Hospital Universitario Central de Asturias, 33011 Oviedo, SpainUnidad de Cuidados Intensivos Cardiológicos, Hospital Universitario Central de Asturias, 33011 Oviedo, SpainServicio de Microbiología Clínica, Hospital Universitario Central de Asturias, 33011 Oviedo, SpainServicio de Microbiología Clínica, Hospital Universitario Central de Asturias, 33011 Oviedo, SpainServicio de Medicina Interna, Hospital Universitario Central de Asturias, 33011 Oviedo, SpainServicio de Microbiología Clínica, Hospital Universitario Central de Asturias, 33011 Oviedo, SpainServicio de Cirugía Cardiaca, Hospital Universitario Central de Asturias, 33011 Oviedo, SpainUnidad de Cuidados Intensivos Cardiológicos, Hospital Universitario Central de Asturias, 33011 Oviedo, SpainInstituto de Investigación Sanitaria del Principado de Asturias (ISPA), 33011 Oviedo, SpainIdentification of the causative pathogen is required to optimize the effective therapy in infective endocarditis (IE). The aim of this study was to assess a 16S rDNA PCR to identify bacteria from heart valve tissues and to evaluate its usefulness as a complement to blood and removed valves cultures. A total of 266 patients diagnosed with IE from January 2015 to December 2019 were evaluated. Results between 16S rDNA PCR from heart valve tissues were compared with microbiological cultures. Blood cultures were positive in 83.5% of patients diagnosed with IE, while 39.6% and 71.8% of the evaluated heart valve samples were positive by culture and 16S rDNA PCR, respectively. For 32 (12%) patients, 16S rDNA tissue PCR provided valuable information supporting the results of blood cultures in the case of bacteria characteristic from the skin microbiota. Additionally, a microorganism was identified by using 16S rDNA PCR in 36% of blood culture-negative cases. The present study reveals that molecular diagnosis using 16S rDNA tissue PCR provides complementary information for the diagnosis of IE, and it should be recommended in surgical endocarditis, especially when blood cultures are negative.https://www.mdpi.com/2075-4418/11/8/1372infective endocarditis16S rDNA PCRblood culturevalve heart culturemolecular diagnosis |
| spellingShingle | Raquel Rodríguez-García María Ángeles Rodríguez-Esteban Jonathan Fernández-Suárez Ana Morilla Enrique García-Carús Mauricio Telenti Carlos Morales Guillermo Muñiz Albaiceta Javier Fernández Evaluation of 16S rDNA Heart Tissue PCR as a Complement to Blood Cultures for the Routine Etiological Diagnosis of Infective Endocarditis Diagnostics infective endocarditis 16S rDNA PCR blood culture valve heart culture molecular diagnosis |
| title | Evaluation of 16S rDNA Heart Tissue PCR as a Complement to Blood Cultures for the Routine Etiological Diagnosis of Infective Endocarditis |
| title_full | Evaluation of 16S rDNA Heart Tissue PCR as a Complement to Blood Cultures for the Routine Etiological Diagnosis of Infective Endocarditis |
| title_fullStr | Evaluation of 16S rDNA Heart Tissue PCR as a Complement to Blood Cultures for the Routine Etiological Diagnosis of Infective Endocarditis |
| title_full_unstemmed | Evaluation of 16S rDNA Heart Tissue PCR as a Complement to Blood Cultures for the Routine Etiological Diagnosis of Infective Endocarditis |
| title_short | Evaluation of 16S rDNA Heart Tissue PCR as a Complement to Blood Cultures for the Routine Etiological Diagnosis of Infective Endocarditis |
| title_sort | evaluation of 16s rdna heart tissue pcr as a complement to blood cultures for the routine etiological diagnosis of infective endocarditis |
| topic | infective endocarditis 16S rDNA PCR blood culture valve heart culture molecular diagnosis |
| url | https://www.mdpi.com/2075-4418/11/8/1372 |
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