Streptococcus pyogenes carriage acquisition, persistence and transmission dynamics within households in The Gambia (SpyCATS): protocol for a longitudinal household cohort study [version 2; peer review: 2 approved]
Background Streptococcus pyogenes (StrepA) causes a significant burden of disease globally from superficial infections to invasive disease. It is responsible for over 500,000 deaths each year, predominantly in low- and middle-income countries (LMIC). Superficial StrepA infections of the skin and pha...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wellcome
2023-10-01
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| Series: | Wellcome Open Research |
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| Online Access: | https://wellcomeopenresearch.org/articles/8-41/v2 |
| _version_ | 1827765158820184064 |
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| author | Edwin P. Armitage Gabrielle de Crombrugghe Alex J. Keeley Fatoumatta E. Camara Elina Senghore Amat Bittaye Musukoi Jammeh Adam Kucharski Isatou Ceesay Haddy Ceesay Muhammed Manneh Bunja Samateh Thushan I. de Silva Abdul Karim Sesay Beate Kampmann Michael Marks |
| author_facet | Edwin P. Armitage Gabrielle de Crombrugghe Alex J. Keeley Fatoumatta E. Camara Elina Senghore Amat Bittaye Musukoi Jammeh Adam Kucharski Isatou Ceesay Haddy Ceesay Muhammed Manneh Bunja Samateh Thushan I. de Silva Abdul Karim Sesay Beate Kampmann Michael Marks |
| author_sort | Edwin P. Armitage |
| collection | DOAJ |
| description | Background Streptococcus pyogenes (StrepA) causes a significant burden of disease globally from superficial infections to invasive disease. It is responsible for over 500,000 deaths each year, predominantly in low- and middle-income countries (LMIC). Superficial StrepA infections of the skin and pharynx can lead to rheumatic heart disease, the largest cause of StrepA-related deaths in LMIC. StrepA can also asymptomatically colonise normal skin and the pharynx (carriage), potentially increasing infection risk. Streptococcus dysgalactiae subsp. equisimilis (SDSE) carriage is also common in LMIC and may interact with StrepA. This study aims to investigate StrepA and SDSE carriage and infection epidemiology, transmission dynamics and naturally acquired immunity within households in The Gambia. Methods A longitudinal household observational cohort study will be conducted over one year. 45 households will be recruited from the urban area of Sukuta, The Gambia, resulting in approximately 450 participants. Households will be visited monthly, and available participants will undergo oropharyngeal and normal skin swabbing. Incident cases of pharyngitis and pyoderma will be captured via active case reporting, with swabs taken from disease sites. Swabs will be cultured for the presence of group A, C and G beta-haemolytic streptococci. Isolates will undergo whole genome sequencing. At each visit, clinical, socio-demographic and social mixing data will be collected. Blood serum will be collected at baseline and final visit. Oral fluid and dried blood spot samples will be collected at each visit. Mucosal and serum anti-StrepA antibody responses will be measured. Outcome This study will report StrepA and SDSE clinical epidemiology, risk factors, transmission dynamics, and serological responses to carriage and infection. Detailed social mixing behaviour will be combined with phylogenetic relatedness to model the extent of transmission occurring withing and between households. The study will provide data to help meet global strategic StrepA research goals. |
| first_indexed | 2024-03-11T11:16:09Z |
| format | Article |
| id | doaj.art-0f0cf0039ad24356afaeb9f92a071cee |
| institution | Directory Open Access Journal |
| issn | 2398-502X |
| language | English |
| last_indexed | 2024-03-11T11:16:09Z |
| publishDate | 2023-10-01 |
| publisher | Wellcome |
| record_format | Article |
| series | Wellcome Open Research |
| spelling | doaj.art-0f0cf0039ad24356afaeb9f92a071cee2023-11-11T01:00:02ZengWellcomeWellcome Open Research2398-502X2023-10-01822555Streptococcus pyogenes carriage acquisition, persistence and transmission dynamics within households in The Gambia (SpyCATS): protocol for a longitudinal household cohort study [version 2; peer review: 2 approved]Edwin P. Armitage0https://orcid.org/0000-0003-1125-4260Gabrielle de Crombrugghe1https://orcid.org/0000-0003-4700-8600Alex J. Keeley2Fatoumatta E. Camara3Elina Senghore4Amat Bittaye5Musukoi Jammeh6Adam Kucharski7Isatou Ceesay8Haddy Ceesay9Muhammed Manneh10Bunja Samateh11Thushan I. de Silva12Abdul Karim Sesay13Beate Kampmann14https://orcid.org/0000-0002-6546-4709Michael Marks15https://orcid.org/0000-0002-7585-4743Vaccines and Immunity Theme, Medical Research Council Unit The Gambia at the London School of Hygiene & Tropical Medicine, Banjul, The GambiaMolecular Bacteriology Laboratory, Faculty of Medicine, Free University of Brussels, Brussels, BelgiumVaccines and Immunity Theme, Medical Research Council Unit The Gambia at the London School of Hygiene & Tropical Medicine, Banjul, The GambiaVaccines and Immunity Theme, Medical Research Council Unit The Gambia at the London School of Hygiene & Tropical Medicine, Banjul, The GambiaVaccines and Immunity Theme, Medical Research Council Unit The Gambia at the London School of Hygiene & Tropical Medicine, Banjul, The GambiaVaccines and Immunity Theme, Medical Research Council Unit The Gambia at the London School of Hygiene & Tropical Medicine, Banjul, The GambiaVaccines and Immunity Theme, Medical Research Council Unit The Gambia at the London School of Hygiene & Tropical Medicine, Banjul, The GambiaCentre for Mathematical Modelling of Infectious Diseases, London School of Hygiene and Tropical Medicine, London, WC1E 7HT, UKVaccines and Immunity Theme, Medical Research Council Unit The Gambia at the London School of Hygiene & Tropical Medicine, Banjul, The GambiaVaccines and Immunity Theme, Medical Research Council Unit The Gambia at the London School of Hygiene & Tropical Medicine, Banjul, The GambiaVaccines and Immunity Theme, Medical Research Council Unit The Gambia at the London School of Hygiene & Tropical Medicine, Banjul, The GambiaVaccines and Immunity Theme, Medical Research Council Unit The Gambia at the London School of Hygiene & Tropical Medicine, Banjul, The GambiaVaccines and Immunity Theme, Medical Research Council Unit The Gambia at the London School of Hygiene & Tropical Medicine, Banjul, The GambiaGenomics Strategic Core Platform, Medical Research Council Unit The Gambia at the London School of Hygiene & Tropical Medicine, Banjul, The GambiaVaccines and Immunity Theme, Medical Research Council Unit The Gambia at the London School of Hygiene & Tropical Medicine, Banjul, The GambiaDivision of Infection and Immunity, University College London, London, WC1E 6BT, UKBackground Streptococcus pyogenes (StrepA) causes a significant burden of disease globally from superficial infections to invasive disease. It is responsible for over 500,000 deaths each year, predominantly in low- and middle-income countries (LMIC). Superficial StrepA infections of the skin and pharynx can lead to rheumatic heart disease, the largest cause of StrepA-related deaths in LMIC. StrepA can also asymptomatically colonise normal skin and the pharynx (carriage), potentially increasing infection risk. Streptococcus dysgalactiae subsp. equisimilis (SDSE) carriage is also common in LMIC and may interact with StrepA. This study aims to investigate StrepA and SDSE carriage and infection epidemiology, transmission dynamics and naturally acquired immunity within households in The Gambia. Methods A longitudinal household observational cohort study will be conducted over one year. 45 households will be recruited from the urban area of Sukuta, The Gambia, resulting in approximately 450 participants. Households will be visited monthly, and available participants will undergo oropharyngeal and normal skin swabbing. Incident cases of pharyngitis and pyoderma will be captured via active case reporting, with swabs taken from disease sites. Swabs will be cultured for the presence of group A, C and G beta-haemolytic streptococci. Isolates will undergo whole genome sequencing. At each visit, clinical, socio-demographic and social mixing data will be collected. Blood serum will be collected at baseline and final visit. Oral fluid and dried blood spot samples will be collected at each visit. Mucosal and serum anti-StrepA antibody responses will be measured. Outcome This study will report StrepA and SDSE clinical epidemiology, risk factors, transmission dynamics, and serological responses to carriage and infection. Detailed social mixing behaviour will be combined with phylogenetic relatedness to model the extent of transmission occurring withing and between households. The study will provide data to help meet global strategic StrepA research goals.https://wellcomeopenresearch.org/articles/8-41/v2Streptococcus pyogenes asymptomatic carriage pharyngitis pyoderma longitudinal cohort study transmission modellingeng |
| spellingShingle | Edwin P. Armitage Gabrielle de Crombrugghe Alex J. Keeley Fatoumatta E. Camara Elina Senghore Amat Bittaye Musukoi Jammeh Adam Kucharski Isatou Ceesay Haddy Ceesay Muhammed Manneh Bunja Samateh Thushan I. de Silva Abdul Karim Sesay Beate Kampmann Michael Marks Streptococcus pyogenes carriage acquisition, persistence and transmission dynamics within households in The Gambia (SpyCATS): protocol for a longitudinal household cohort study [version 2; peer review: 2 approved] Wellcome Open Research Streptococcus pyogenes asymptomatic carriage pharyngitis pyoderma longitudinal cohort study transmission modelling eng |
| title | Streptococcus pyogenes carriage acquisition, persistence and transmission dynamics within households in The Gambia (SpyCATS): protocol for a longitudinal household cohort study [version 2; peer review: 2 approved] |
| title_full | Streptococcus pyogenes carriage acquisition, persistence and transmission dynamics within households in The Gambia (SpyCATS): protocol for a longitudinal household cohort study [version 2; peer review: 2 approved] |
| title_fullStr | Streptococcus pyogenes carriage acquisition, persistence and transmission dynamics within households in The Gambia (SpyCATS): protocol for a longitudinal household cohort study [version 2; peer review: 2 approved] |
| title_full_unstemmed | Streptococcus pyogenes carriage acquisition, persistence and transmission dynamics within households in The Gambia (SpyCATS): protocol for a longitudinal household cohort study [version 2; peer review: 2 approved] |
| title_short | Streptococcus pyogenes carriage acquisition, persistence and transmission dynamics within households in The Gambia (SpyCATS): protocol for a longitudinal household cohort study [version 2; peer review: 2 approved] |
| title_sort | streptococcus pyogenes carriage acquisition persistence and transmission dynamics within households in the gambia spycats protocol for a longitudinal household cohort study version 2 peer review 2 approved |
| topic | Streptococcus pyogenes asymptomatic carriage pharyngitis pyoderma longitudinal cohort study transmission modelling eng |
| url | https://wellcomeopenresearch.org/articles/8-41/v2 |
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