<i>Lonicera caerulea</i> Pomace Alleviates DSS-Induced Colitis via Intestinal Barrier Improvement and Gut Microbiota Modulation
The objective of this investigation was to appraise the mitigative effects of <i>Lonicera caerulea</i> pomace (LCP)-supplemented diets on Dextran Sulfate Sodium (DSS)-induced colitis, and to discuss the potential mechanisms. LCP, a by-product of fruit juice processing, harbors a higher c...
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MDPI AG
2023-09-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/18/3329 |
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| author | Baixi Zhang Xinwen Huang Lijuan Niu Xuemei Chen Bo Hu Xiaoshu Tang |
| author_facet | Baixi Zhang Xinwen Huang Lijuan Niu Xuemei Chen Bo Hu Xiaoshu Tang |
| author_sort | Baixi Zhang |
| collection | DOAJ |
| description | The objective of this investigation was to appraise the mitigative effects of <i>Lonicera caerulea</i> pomace (LCP)-supplemented diets on Dextran Sulfate Sodium (DSS)-induced colitis, and to discuss the potential mechanisms. LCP, a by-product of fruit juice processing, harbors a higher content of polyphenols and dietary fiber compared to the <i>L. caerulea</i> pulp. In a murine model of colitis, the LCP diet attenuated the symptoms of colitis, as evidenced by the reduction in the disease activity index (DAI), extension of colon length, and amelioration of histopathological damage. The anti-inflammatory attributes of LCP were substantiated by a decrease in myeloperoxidase (MPO) activity and suppression of inflammatory cytokine expressions within the colon. Meanwhile, LCP mediated the repair of the intestinal barrier, characterized by the upregulation of gene expressions of tight junction (TJ) proteins and <i>Muc2</i>. Furthermore, LCP altered the composition of the gut microbiota, manifested in increased alpha diversity, enhancement of the abundance of beneficial bacteria (<i>Akkermansia</i>, <i>Coprococcus</i> and <i>Bifidobacterium</i>), and diminishment in the abundance of pathogenic bacteria (<i>Escherichia</i>, <i>Enterococcus</i>, <i>Mucispirillum</i> and <i>Clostridium</i>). Dietary LCP also increased the concentrations of SCFAs within the intestinal luminal contents of colitis mice. Given the affirmative impact of LCP on colitis, LCP may possess great potential in promoting intestinal health. |
| first_indexed | 2024-03-10T22:45:57Z |
| format | Article |
| id | doaj.art-0f0ee747c66e4f1e874ad0a877471c45 |
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| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T22:45:57Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-0f0ee747c66e4f1e874ad0a877471c452023-11-19T10:41:31ZengMDPI AGFoods2304-81582023-09-011218332910.3390/foods12183329<i>Lonicera caerulea</i> Pomace Alleviates DSS-Induced Colitis via Intestinal Barrier Improvement and Gut Microbiota ModulationBaixi Zhang0Xinwen Huang1Lijuan Niu2Xuemei Chen3Bo Hu4Xiaoshu Tang5School of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaSchool of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaSchool of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaSchool of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaSchool of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaSchool of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaThe objective of this investigation was to appraise the mitigative effects of <i>Lonicera caerulea</i> pomace (LCP)-supplemented diets on Dextran Sulfate Sodium (DSS)-induced colitis, and to discuss the potential mechanisms. LCP, a by-product of fruit juice processing, harbors a higher content of polyphenols and dietary fiber compared to the <i>L. caerulea</i> pulp. In a murine model of colitis, the LCP diet attenuated the symptoms of colitis, as evidenced by the reduction in the disease activity index (DAI), extension of colon length, and amelioration of histopathological damage. The anti-inflammatory attributes of LCP were substantiated by a decrease in myeloperoxidase (MPO) activity and suppression of inflammatory cytokine expressions within the colon. Meanwhile, LCP mediated the repair of the intestinal barrier, characterized by the upregulation of gene expressions of tight junction (TJ) proteins and <i>Muc2</i>. Furthermore, LCP altered the composition of the gut microbiota, manifested in increased alpha diversity, enhancement of the abundance of beneficial bacteria (<i>Akkermansia</i>, <i>Coprococcus</i> and <i>Bifidobacterium</i>), and diminishment in the abundance of pathogenic bacteria (<i>Escherichia</i>, <i>Enterococcus</i>, <i>Mucispirillum</i> and <i>Clostridium</i>). Dietary LCP also increased the concentrations of SCFAs within the intestinal luminal contents of colitis mice. Given the affirmative impact of LCP on colitis, LCP may possess great potential in promoting intestinal health.https://www.mdpi.com/2304-8158/12/18/3329<i>Lonicera caerulea</i>ulcerative colitisanti-inflammatoryintestinal barriergut microbiota |
| spellingShingle | Baixi Zhang Xinwen Huang Lijuan Niu Xuemei Chen Bo Hu Xiaoshu Tang <i>Lonicera caerulea</i> Pomace Alleviates DSS-Induced Colitis via Intestinal Barrier Improvement and Gut Microbiota Modulation Foods <i>Lonicera caerulea</i> ulcerative colitis anti-inflammatory intestinal barrier gut microbiota |
| title | <i>Lonicera caerulea</i> Pomace Alleviates DSS-Induced Colitis via Intestinal Barrier Improvement and Gut Microbiota Modulation |
| title_full | <i>Lonicera caerulea</i> Pomace Alleviates DSS-Induced Colitis via Intestinal Barrier Improvement and Gut Microbiota Modulation |
| title_fullStr | <i>Lonicera caerulea</i> Pomace Alleviates DSS-Induced Colitis via Intestinal Barrier Improvement and Gut Microbiota Modulation |
| title_full_unstemmed | <i>Lonicera caerulea</i> Pomace Alleviates DSS-Induced Colitis via Intestinal Barrier Improvement and Gut Microbiota Modulation |
| title_short | <i>Lonicera caerulea</i> Pomace Alleviates DSS-Induced Colitis via Intestinal Barrier Improvement and Gut Microbiota Modulation |
| title_sort | i lonicera caerulea i pomace alleviates dss induced colitis via intestinal barrier improvement and gut microbiota modulation |
| topic | <i>Lonicera caerulea</i> ulcerative colitis anti-inflammatory intestinal barrier gut microbiota |
| url | https://www.mdpi.com/2304-8158/12/18/3329 |
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