Advances and Remaining Challenges in the Study of Influenza and Anthrax Infection in Lung Cell Culture
For over 30 years, immortalized lung cells have enabled researchers to elucidate lung-pathogen molecular interactions. However, over the last five years, numerous commercial companies are now providing affordable, ready-to-use primary lung cells for use in research laboratories. Despite advances in...
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-01-01
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| Series: | Challenges |
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| Online Access: | http://www.mdpi.com/2078-1547/9/1/2 |
| _version_ | 1818487156807565312 |
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| author | Joshua D. Powell Timothy M. Straub |
| author_facet | Joshua D. Powell Timothy M. Straub |
| author_sort | Joshua D. Powell |
| collection | DOAJ |
| description | For over 30 years, immortalized lung cells have enabled researchers to elucidate lung-pathogen molecular interactions. However, over the last five years, numerous commercial companies are now providing affordable, ready-to-use primary lung cells for use in research laboratories. Despite advances in primary cell culture, studies using immortalized lung cells still dominate the recent scientific literature. In this review, we highlight recent influenza and anthrax studies using in vitro primary lung tissue models and how these models are providing better predictive outcomes for when extrapolated to in vivo observations. By focusing on one virus (influenza) and one bacterium (Bacillus anthracis), it is the intent that these primary lung cell culture observations may translate into more useful studies for other related viral and bacterial lung pathogens of interest. |
| first_indexed | 2024-12-10T16:33:55Z |
| format | Article |
| id | doaj.art-0f0fd808a29144d1a612772377c13dcf |
| institution | Directory Open Access Journal |
| issn | 2078-1547 |
| language | English |
| last_indexed | 2024-12-10T16:33:55Z |
| publishDate | 2018-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Challenges |
| spelling | doaj.art-0f0fd808a29144d1a612772377c13dcf2022-12-22T01:41:29ZengMDPI AGChallenges2078-15472018-01-0191210.3390/challe9010002challe9010002Advances and Remaining Challenges in the Study of Influenza and Anthrax Infection in Lung Cell CultureJoshua D. Powell0Timothy M. Straub1Chemical and Biological Signature Sciences Group, Pacific Northwest National Laboratory, Richland, WA 99352, USAChemical and Biological Signature Sciences Group, Pacific Northwest National Laboratory, Richland, WA 99352, USAFor over 30 years, immortalized lung cells have enabled researchers to elucidate lung-pathogen molecular interactions. However, over the last five years, numerous commercial companies are now providing affordable, ready-to-use primary lung cells for use in research laboratories. Despite advances in primary cell culture, studies using immortalized lung cells still dominate the recent scientific literature. In this review, we highlight recent influenza and anthrax studies using in vitro primary lung tissue models and how these models are providing better predictive outcomes for when extrapolated to in vivo observations. By focusing on one virus (influenza) and one bacterium (Bacillus anthracis), it is the intent that these primary lung cell culture observations may translate into more useful studies for other related viral and bacterial lung pathogens of interest.http://www.mdpi.com/2078-1547/9/1/2anthraxinfluenzalung epithelialNHBE |
| spellingShingle | Joshua D. Powell Timothy M. Straub Advances and Remaining Challenges in the Study of Influenza and Anthrax Infection in Lung Cell Culture Challenges anthrax influenza lung epithelial NHBE |
| title | Advances and Remaining Challenges in the Study of Influenza and Anthrax Infection in Lung Cell Culture |
| title_full | Advances and Remaining Challenges in the Study of Influenza and Anthrax Infection in Lung Cell Culture |
| title_fullStr | Advances and Remaining Challenges in the Study of Influenza and Anthrax Infection in Lung Cell Culture |
| title_full_unstemmed | Advances and Remaining Challenges in the Study of Influenza and Anthrax Infection in Lung Cell Culture |
| title_short | Advances and Remaining Challenges in the Study of Influenza and Anthrax Infection in Lung Cell Culture |
| title_sort | advances and remaining challenges in the study of influenza and anthrax infection in lung cell culture |
| topic | anthrax influenza lung epithelial NHBE |
| url | http://www.mdpi.com/2078-1547/9/1/2 |
| work_keys_str_mv | AT joshuadpowell advancesandremainingchallengesinthestudyofinfluenzaandanthraxinfectioninlungcellculture AT timothymstraub advancesandremainingchallengesinthestudyofinfluenzaandanthraxinfectioninlungcellculture |