Value of Rapid Antigen Test in Comparison with Reverse Transcriptase-Polymerase Chain Reaction Method in the Diagnosis of COVID-19
Introduction: Using the rapid antigen test (RAT) before exhausting the reverse transcriptase-polymerase chain reaction (RT-PCR) test’s capacity is crucial to enhance suitable detection of patients and timely reception of results. Therefore, this study was done to evaluate the sensitivity and specifi...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | Turkish |
| Published: |
Galenos Yayinevi
2022-03-01
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| Series: | Mediterranean Journal of Infection, Microbes and Antimicrobials |
| Subjects: | |
| Online Access: | http://mjima.org/text.php?&id=299 |
| _version_ | 1797911288444092416 |
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| author | Alireza MIRAHMADIZADEH Babak Shirazi YEGANEH Jafar HASSANZADEH Leila BADIEE Mohebat VALI Zahra MALEKI Ali AKBARI Tahereh PAKDEL Haleh GHAEM |
| author_facet | Alireza MIRAHMADIZADEH Babak Shirazi YEGANEH Jafar HASSANZADEH Leila BADIEE Mohebat VALI Zahra MALEKI Ali AKBARI Tahereh PAKDEL Haleh GHAEM |
| author_sort | Alireza MIRAHMADIZADEH |
| collection | DOAJ |
| description | Introduction: Using the rapid antigen test (RAT) before exhausting the reverse transcriptase-polymerase chain reaction (RT-PCR) test’s capacity is crucial to enhance suitable detection of patients and timely reception of results. Therefore, this study was done to evaluate the sensitivity and specificity of RAT and compare it with the RT-PCR method in the diagnosis of Coronavirus disease-2019 (COVID-19).
Materials and Methods: This study was performed on 634 individuals referred to public sampling centers performing the COVID-19 test in Shiraz City, Fars Province, Southern Iran. The sampling process was done following a multi-stage stratified protocol. The COVITECH® one-step real-time RT-PCR kit method as the reference standard test was compared with the RAT using E-Health Barakat Company® rapid antigen kit in the pharyngeal specimens. The trained personnel collected the data. Sensitivity, specificity, positive predictive value (PPV), negative predictive value, and the
accuracy of the RAT were calculated using the MedCalc software. Moreover, the Kappa value was used to assess the level of agreement between RT-PCR and RAT.
Results: According to the results of the RT-PCR method as a reference test; sensitivity, specificity, Kappa value, and accuracy of the RAT were 81.82%, 92.28%, 63.8%, and 91.01%, respectively in the diagnosis of COVID-19. In asymptomatic individuals, the specificity and the PPV of the RAT were 100%.
Conclusion: In conclusion, our results suggested that a positive RAT provides considerable information for diagnosing COVID-19 in adults. As a result, in general, due to the high sensitivity, specificity, and accuracy of the RAT, it can be replaced with the RT-PCR test considering factors such as time, cost, and speed. |
| first_indexed | 2024-04-10T11:38:13Z |
| format | Article |
| id | doaj.art-0f12f184696246308bef63ac9287235b |
| institution | Directory Open Access Journal |
| issn | 2147-673X |
| language | Turkish |
| last_indexed | 2024-04-10T11:38:13Z |
| publishDate | 2022-03-01 |
| publisher | Galenos Yayinevi |
| record_format | Article |
| series | Mediterranean Journal of Infection, Microbes and Antimicrobials |
| spelling | doaj.art-0f12f184696246308bef63ac9287235b2023-02-15T16:17:37ZturGalenos YayineviMediterranean Journal of Infection, Microbes and Antimicrobials2147-673X2022-03-0111110.4274/mjima.galenos.2021.2021.2Value of Rapid Antigen Test in Comparison with Reverse Transcriptase-Polymerase Chain Reaction Method in the Diagnosis of COVID-19Alireza MIRAHMADIZADEH0https://orcid.org/0000-0002-2259-4984Babak Shirazi YEGANEH1https://orcid.org/0000-0001-6373-4376Jafar HASSANZADEH2https://orcid.org/0000-0002-1055-1418Leila BADIEE3https://orcid.org/0000-0003-0620-9449Mohebat VALI4https://orcid.org/0000-0001-7733-2116Zahra MALEKI5https://orcid.org/0000-0001-6919-9239Ali AKBARI6https://orcid.org/0000-0002-2970-2052Tahereh PAKDEL7https://orcid.org/0000-0002-8463-5868Haleh GHAEM8https://orcid.org/0000-0001-9564-392XNon-Communicable Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, IranShiraz University of Medical Sciences, Faculty of Medicine, Department of Pathology, Shiraz, IranShiraz University of Medical Sciences, Department of Epidemiology, Shiraz, IranShiraz University of Medical Sciences, Head of Laboratories of Health Affair, Shiraz, IranShiraz University of Medical Sciences, Student Research Committee, Shiraz, IranShiraz University of Medical Sciences, Student Research Committee, Shiraz, IranShiraz University of Medical Sciences, Faculty of Medicine, Department of Anesthesiology, Shiraz, IranShiraz University of Medical Sciences, Expert in Laboratory Diagnosis, Shiraz, IranNon-Communicable Diseases Research Center, Research Center for Health Sciences, Institute of Health, Department of Epidemiology, School of Health, Shiraz University of Medical Sciences, Shiraz, IranIntroduction: Using the rapid antigen test (RAT) before exhausting the reverse transcriptase-polymerase chain reaction (RT-PCR) test’s capacity is crucial to enhance suitable detection of patients and timely reception of results. Therefore, this study was done to evaluate the sensitivity and specificity of RAT and compare it with the RT-PCR method in the diagnosis of Coronavirus disease-2019 (COVID-19). Materials and Methods: This study was performed on 634 individuals referred to public sampling centers performing the COVID-19 test in Shiraz City, Fars Province, Southern Iran. The sampling process was done following a multi-stage stratified protocol. The COVITECH® one-step real-time RT-PCR kit method as the reference standard test was compared with the RAT using E-Health Barakat Company® rapid antigen kit in the pharyngeal specimens. The trained personnel collected the data. Sensitivity, specificity, positive predictive value (PPV), negative predictive value, and the accuracy of the RAT were calculated using the MedCalc software. Moreover, the Kappa value was used to assess the level of agreement between RT-PCR and RAT. Results: According to the results of the RT-PCR method as a reference test; sensitivity, specificity, Kappa value, and accuracy of the RAT were 81.82%, 92.28%, 63.8%, and 91.01%, respectively in the diagnosis of COVID-19. In asymptomatic individuals, the specificity and the PPV of the RAT were 100%. Conclusion: In conclusion, our results suggested that a positive RAT provides considerable information for diagnosing COVID-19 in adults. As a result, in general, due to the high sensitivity, specificity, and accuracy of the RAT, it can be replaced with the RT-PCR test considering factors such as time, cost, and speed.http://mjima.org/text.php?&id=299covid-19reverse transcriptase polymerase chain reactionsensitivityspecificityrapid |
| spellingShingle | Alireza MIRAHMADIZADEH Babak Shirazi YEGANEH Jafar HASSANZADEH Leila BADIEE Mohebat VALI Zahra MALEKI Ali AKBARI Tahereh PAKDEL Haleh GHAEM Value of Rapid Antigen Test in Comparison with Reverse Transcriptase-Polymerase Chain Reaction Method in the Diagnosis of COVID-19 Mediterranean Journal of Infection, Microbes and Antimicrobials covid-19 reverse transcriptase polymerase chain reaction sensitivity specificity rapid |
| title | Value of Rapid Antigen Test in Comparison with Reverse Transcriptase-Polymerase Chain Reaction Method in the Diagnosis of COVID-19 |
| title_full | Value of Rapid Antigen Test in Comparison with Reverse Transcriptase-Polymerase Chain Reaction Method in the Diagnosis of COVID-19 |
| title_fullStr | Value of Rapid Antigen Test in Comparison with Reverse Transcriptase-Polymerase Chain Reaction Method in the Diagnosis of COVID-19 |
| title_full_unstemmed | Value of Rapid Antigen Test in Comparison with Reverse Transcriptase-Polymerase Chain Reaction Method in the Diagnosis of COVID-19 |
| title_short | Value of Rapid Antigen Test in Comparison with Reverse Transcriptase-Polymerase Chain Reaction Method in the Diagnosis of COVID-19 |
| title_sort | value of rapid antigen test in comparison with reverse transcriptase polymerase chain reaction method in the diagnosis of covid 19 |
| topic | covid-19 reverse transcriptase polymerase chain reaction sensitivity specificity rapid |
| url | http://mjima.org/text.php?&id=299 |
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