Protocol for using autoclaved intertidal sediment as a medium to enrich marine cable bacteria
Summary: Cable bacteria (CB) are non-isolated filamentous bacteria in the family of Desulfobulbaceae, known for fostering centimeter-long electron transfer in sediments with pronounced redox zonation. This protocol details steps to extract CB filaments from cultured natural sediment, inoculate autoc...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-09-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722004841 |
| _version_ | 1811310646433677312 |
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| author | Cheng Li Clare E. Reimers Peter J. Chace |
| author_facet | Cheng Li Clare E. Reimers Peter J. Chace |
| author_sort | Cheng Li |
| collection | DOAJ |
| description | Summary: Cable bacteria (CB) are non-isolated filamentous bacteria in the family of Desulfobulbaceae, known for fostering centimeter-long electron transfer in sediments with pronounced redox zonation. This protocol details steps to extract CB filaments from cultured natural sediment, inoculate autoclaved sediment with extracted filaments, and subsequently evaluate the growth and enrichment of CB. We also describe the approaches for collecting suitable sediment, preparing autoclaved sediment, and manufacturing glass needles and hooks for the extraction of CB. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-13T10:02:34Z |
| format | Article |
| id | doaj.art-0f13aad436fc4ed491e0ff9d75bdd35b |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-13T10:02:34Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-0f13aad436fc4ed491e0ff9d75bdd35b2022-12-22T02:51:11ZengElsevierSTAR Protocols2666-16672022-09-0133101604Protocol for using autoclaved intertidal sediment as a medium to enrich marine cable bacteriaCheng Li0Clare E. Reimers1Peter J. Chace2College of Earth, Ocean, and Atmospheric Science, Oregon State University, Corvallis, OR 97333, USA; Corresponding authorCollege of Earth, Ocean, and Atmospheric Science, Oregon State University, Corvallis, OR 97333, USACollege of Earth, Ocean, and Atmospheric Science, Oregon State University, Corvallis, OR 97333, USASummary: Cable bacteria (CB) are non-isolated filamentous bacteria in the family of Desulfobulbaceae, known for fostering centimeter-long electron transfer in sediments with pronounced redox zonation. This protocol details steps to extract CB filaments from cultured natural sediment, inoculate autoclaved sediment with extracted filaments, and subsequently evaluate the growth and enrichment of CB. We also describe the approaches for collecting suitable sediment, preparing autoclaved sediment, and manufacturing glass needles and hooks for the extraction of CB. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166722004841Cell cultureMicrobiologyEnvironmental sciences |
| spellingShingle | Cheng Li Clare E. Reimers Peter J. Chace Protocol for using autoclaved intertidal sediment as a medium to enrich marine cable bacteria STAR Protocols Cell culture Microbiology Environmental sciences |
| title | Protocol for using autoclaved intertidal sediment as a medium to enrich marine cable bacteria |
| title_full | Protocol for using autoclaved intertidal sediment as a medium to enrich marine cable bacteria |
| title_fullStr | Protocol for using autoclaved intertidal sediment as a medium to enrich marine cable bacteria |
| title_full_unstemmed | Protocol for using autoclaved intertidal sediment as a medium to enrich marine cable bacteria |
| title_short | Protocol for using autoclaved intertidal sediment as a medium to enrich marine cable bacteria |
| title_sort | protocol for using autoclaved intertidal sediment as a medium to enrich marine cable bacteria |
| topic | Cell culture Microbiology Environmental sciences |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722004841 |
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