Characterization of the CCAAT-binding transcription factor complex in the plant pathogenic fungus Fusarium graminearum
Abstract The CCAAT sequence is a ubiquitous cis-element of eukaryotic promoters, and genes containing CCAAT sequences have been shown to be activated by the CCAAT-binding transcription factor complex in several eukaryotic model organisms. In general, CCAAT-binding transcription factors form heterodi...
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| Format: | Article |
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Nature Portfolio
2020-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-020-61885-4 |
| _version_ | 1818842886650724352 |
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| author | Jung-Eun Kim Hyejin Nam Jiyeun Park Gyung Ja Choi Yin-Won Lee Hokyoung Son |
| author_facet | Jung-Eun Kim Hyejin Nam Jiyeun Park Gyung Ja Choi Yin-Won Lee Hokyoung Son |
| author_sort | Jung-Eun Kim |
| collection | DOAJ |
| description | Abstract The CCAAT sequence is a ubiquitous cis-element of eukaryotic promoters, and genes containing CCAAT sequences have been shown to be activated by the CCAAT-binding transcription factor complex in several eukaryotic model organisms. In general, CCAAT-binding transcription factors form heterodimers or heterotrimeric complexes that bind to CCAAT sequences within the promoters of target genes and regulate various cellular processes. To date, except Hap complex, CCAAT-binding complex has been rarely reported in fungi. In this study, we characterized two CCAAT-binding transcription factors (Fct1 and Fct2) in the plant pathogenic fungus Fusarium graminearum. Previously, FCT1 and FCT2 were shown to be related to DNA damage response among eight CCAAT-binding transcription factors in F. graminearum. We demonstrate that the nuclear CCAAT-binding complex of F. graminearum has important functions in various fungal developmental processes, not just DNA damage response but virulence and mycotoxin production. Moreover, the results of biochemical and genetic analyses revealed that Fct1 and Fct2 may form a complex and play distinct roles among the eight CCAAT-binding transcription factors encoded by F. graminearum. To the best of our knowledge, the results of this study represent a substantial advancement in our understanding of the molecular mechanisms underlying the functions of CCAAT-binding factors in eukaryotes. |
| first_indexed | 2024-12-19T04:49:06Z |
| format | Article |
| id | doaj.art-0f13bbf48a034e59babef88b74cfb93e |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-19T04:49:06Z |
| publishDate | 2020-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-0f13bbf48a034e59babef88b74cfb93e2022-12-21T20:35:24ZengNature PortfolioScientific Reports2045-23222020-03-0110111110.1038/s41598-020-61885-4Characterization of the CCAAT-binding transcription factor complex in the plant pathogenic fungus Fusarium graminearumJung-Eun Kim0Hyejin Nam1Jiyeun Park2Gyung Ja Choi3Yin-Won Lee4Hokyoung Son5Research Institute of Agriculture and Life Sciences and Department of Agricultural Biotechnology, Seoul National UniversityResearch Institute of Agriculture and Life Sciences and Department of Agricultural Biotechnology, Seoul National UniversityResearch Institute of Agriculture and Life Sciences and Department of Agricultural Biotechnology, Seoul National UniversityTherapeutic & Biotechnology Division, Center for Eco-friendly New Materials, Korea Research Institute of Chemical TechnologyResearch Institute of Agriculture and Life Sciences and Department of Agricultural Biotechnology, Seoul National UniversityResearch Institute of Agriculture and Life Sciences and Department of Agricultural Biotechnology, Seoul National UniversityAbstract The CCAAT sequence is a ubiquitous cis-element of eukaryotic promoters, and genes containing CCAAT sequences have been shown to be activated by the CCAAT-binding transcription factor complex in several eukaryotic model organisms. In general, CCAAT-binding transcription factors form heterodimers or heterotrimeric complexes that bind to CCAAT sequences within the promoters of target genes and regulate various cellular processes. To date, except Hap complex, CCAAT-binding complex has been rarely reported in fungi. In this study, we characterized two CCAAT-binding transcription factors (Fct1 and Fct2) in the plant pathogenic fungus Fusarium graminearum. Previously, FCT1 and FCT2 were shown to be related to DNA damage response among eight CCAAT-binding transcription factors in F. graminearum. We demonstrate that the nuclear CCAAT-binding complex of F. graminearum has important functions in various fungal developmental processes, not just DNA damage response but virulence and mycotoxin production. Moreover, the results of biochemical and genetic analyses revealed that Fct1 and Fct2 may form a complex and play distinct roles among the eight CCAAT-binding transcription factors encoded by F. graminearum. To the best of our knowledge, the results of this study represent a substantial advancement in our understanding of the molecular mechanisms underlying the functions of CCAAT-binding factors in eukaryotes.https://doi.org/10.1038/s41598-020-61885-4 |
| spellingShingle | Jung-Eun Kim Hyejin Nam Jiyeun Park Gyung Ja Choi Yin-Won Lee Hokyoung Son Characterization of the CCAAT-binding transcription factor complex in the plant pathogenic fungus Fusarium graminearum Scientific Reports |
| title | Characterization of the CCAAT-binding transcription factor complex in the plant pathogenic fungus Fusarium graminearum |
| title_full | Characterization of the CCAAT-binding transcription factor complex in the plant pathogenic fungus Fusarium graminearum |
| title_fullStr | Characterization of the CCAAT-binding transcription factor complex in the plant pathogenic fungus Fusarium graminearum |
| title_full_unstemmed | Characterization of the CCAAT-binding transcription factor complex in the plant pathogenic fungus Fusarium graminearum |
| title_short | Characterization of the CCAAT-binding transcription factor complex in the plant pathogenic fungus Fusarium graminearum |
| title_sort | characterization of the ccaat binding transcription factor complex in the plant pathogenic fungus fusarium graminearum |
| url | https://doi.org/10.1038/s41598-020-61885-4 |
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