Extraction of Squilla (Harpiosquilla annandalei) shell derived chitosan and its nanocarrier efficiency for sustained protein delivery
The delivery of proteins has enormous potential in chronic disease treatment and immunomodulation. But, because of the intrinsic properties of proteins such as poor stability and quick bio clearance, their usage is limited in targeted delivery. This work suggests a cost-effective method to prepare a...
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| Format: | Article |
| Language: | English |
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Bangladesh Society for Microbiology, Immunology, and Advanced Biotechnology
2022-12-01
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| Series: | Journal of Advanced Biotechnology and Experimental Therapeutics |
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| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=22303 |
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| author | Akshad Balde Bhavika Waghela Nazeer Rasool Abdul |
| author_facet | Akshad Balde Bhavika Waghela Nazeer Rasool Abdul |
| author_sort | Akshad Balde |
| collection | DOAJ |
| description | The delivery of proteins has enormous potential in chronic disease treatment and immunomodulation. But, because of the intrinsic properties of proteins such as poor stability and quick bio clearance, their usage is limited in targeted delivery. This work suggests a cost-effective method to prepare an efficient polymer-based nanocarrier system for the sustained delivery of protein. Herein, low molecular weight chitosan(CS) was extracted from the shell waste of squilla (Harpiosquilla annandalei) (sCS)and characterized. It showed higher water-binding capacity (WBC) of 548 ± 11.7% as well as fat binding capacity (FBC) of 369 ± 19.9% respectively, as compared to commercial CS (cCS).BSA loaded nanoparticles were synthesized through the ionic gelation method using cCS and sCS.The sCS-based BSAencapsulated nanoparticles (BSA-sCSNPs) were observed to be uniform and small (60-195 nm) as compared to BSA loadedcCS-based nanoparticles (BSA-cCSNPs). The encapsulation efficiency (EE%) was highest for 1 mg/mL CS, BSA, and tripolyphosphate (TPP) based BSA-sCSNPs. Moreover, the in vitro BSA release profile exhibited that BSA-sCSNPs resulted in more stable and sustained release till the 16th h (85.4 ± 1.15%) than BSA-cCSNPs. Further, the sCS and BSA-sCSNPs were biocompatible with L929 cells and showed no cytotoxic effects. Hence, this biocompatible nanoparticle system can be used as a pharmaceutical as well as a nutraceutical agent to improve the stability as well as targeted delivery of the protein. [ J Adv Biotechnol Exp Ther 2022; 5(3.000): 473-486] |
| first_indexed | 2024-04-13T17:59:30Z |
| format | Article |
| id | doaj.art-0f145d56584b47a58064ad563d851483 |
| institution | Directory Open Access Journal |
| issn | 2616-4760 |
| language | English |
| last_indexed | 2024-04-13T17:59:30Z |
| publishDate | 2022-12-01 |
| publisher | Bangladesh Society for Microbiology, Immunology, and Advanced Biotechnology |
| record_format | Article |
| series | Journal of Advanced Biotechnology and Experimental Therapeutics |
| spelling | doaj.art-0f145d56584b47a58064ad563d8514832022-12-22T02:36:17ZengBangladesh Society for Microbiology, Immunology, and Advanced BiotechnologyJournal of Advanced Biotechnology and Experimental Therapeutics2616-47602022-12-015347348610.5455/jabet.2022.d12922303Extraction of Squilla (Harpiosquilla annandalei) shell derived chitosan and its nanocarrier efficiency for sustained protein deliveryAkshad Balde0Bhavika Waghela1Nazeer Rasool Abdul2Biopharmaceuticals Lab, Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur-603203, Chennai, Tamilnadu, India Biopharmaceuticals Lab, Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur-603203, Chennai, Tamilnadu, India Biopharmaceuticals Lab, Department of Biotechnology, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur-603203, Chennai, Tamilnadu, IndiaThe delivery of proteins has enormous potential in chronic disease treatment and immunomodulation. But, because of the intrinsic properties of proteins such as poor stability and quick bio clearance, their usage is limited in targeted delivery. This work suggests a cost-effective method to prepare an efficient polymer-based nanocarrier system for the sustained delivery of protein. Herein, low molecular weight chitosan(CS) was extracted from the shell waste of squilla (Harpiosquilla annandalei) (sCS)and characterized. It showed higher water-binding capacity (WBC) of 548 ± 11.7% as well as fat binding capacity (FBC) of 369 ± 19.9% respectively, as compared to commercial CS (cCS).BSA loaded nanoparticles were synthesized through the ionic gelation method using cCS and sCS.The sCS-based BSAencapsulated nanoparticles (BSA-sCSNPs) were observed to be uniform and small (60-195 nm) as compared to BSA loadedcCS-based nanoparticles (BSA-cCSNPs). The encapsulation efficiency (EE%) was highest for 1 mg/mL CS, BSA, and tripolyphosphate (TPP) based BSA-sCSNPs. Moreover, the in vitro BSA release profile exhibited that BSA-sCSNPs resulted in more stable and sustained release till the 16th h (85.4 ± 1.15%) than BSA-cCSNPs. Further, the sCS and BSA-sCSNPs were biocompatible with L929 cells and showed no cytotoxic effects. Hence, this biocompatible nanoparticle system can be used as a pharmaceutical as well as a nutraceutical agent to improve the stability as well as targeted delivery of the protein. [ J Adv Biotechnol Exp Ther 2022; 5(3.000): 473-486]http://www.ejmanager.com/fulltextpdf.php?mno=22303chitosanionic gelationmarine wastenanoparticlesprotein delivery |
| spellingShingle | Akshad Balde Bhavika Waghela Nazeer Rasool Abdul Extraction of Squilla (Harpiosquilla annandalei) shell derived chitosan and its nanocarrier efficiency for sustained protein delivery Journal of Advanced Biotechnology and Experimental Therapeutics chitosan ionic gelation marine waste nanoparticles protein delivery |
| title | Extraction of Squilla (Harpiosquilla annandalei) shell derived chitosan and its nanocarrier efficiency for sustained protein delivery |
| title_full | Extraction of Squilla (Harpiosquilla annandalei) shell derived chitosan and its nanocarrier efficiency for sustained protein delivery |
| title_fullStr | Extraction of Squilla (Harpiosquilla annandalei) shell derived chitosan and its nanocarrier efficiency for sustained protein delivery |
| title_full_unstemmed | Extraction of Squilla (Harpiosquilla annandalei) shell derived chitosan and its nanocarrier efficiency for sustained protein delivery |
| title_short | Extraction of Squilla (Harpiosquilla annandalei) shell derived chitosan and its nanocarrier efficiency for sustained protein delivery |
| title_sort | extraction of squilla harpiosquilla annandalei shell derived chitosan and its nanocarrier efficiency for sustained protein delivery |
| topic | chitosan ionic gelation marine waste nanoparticles protein delivery |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=22303 |
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