Clinicoepidemiological study of dengue positive patients admitted to tertiary care hospitals in the Burdwan district, using molecular seroprofiling and enzyme linked immunosorbent assays of cytokines and chemokines
Background: The World Health Organization views dengue fever as a significant worldwide public health threat in the tropics and subtropics. Dengue virus has four serotypes, DENG 1 to 4, which were responsible for outbreaks that occurred in other parts of the world from the 1980s until now. Aims a...
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| Format: | Article |
| Language: | English |
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Manipal College of Medical Sciences, Pokhara
2023-05-01
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| Series: | Asian Journal of Medical Sciences |
| Subjects: | |
| Online Access: | https://www.nepjol.info/index.php/AJMS/article/view/52001 |
| _version_ | 1797835722142515200 |
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| author | Nabamita Chaudhury Tanusri Biswas Swati Nayek Arghya Nath Nivedita Mukherjee Soumi Nag Suraj Mondal Kuntal Das |
| author_facet | Nabamita Chaudhury Tanusri Biswas Swati Nayek Arghya Nath Nivedita Mukherjee Soumi Nag Suraj Mondal Kuntal Das |
| author_sort | Nabamita Chaudhury |
| collection | DOAJ |
| description | Background: The World Health Organization views dengue fever as a significant worldwide public health threat in the tropics and subtropics. Dengue virus has four serotypes, DENG 1 to 4, which were responsible for outbreaks that occurred in other parts of the world from the 1980s until now.
Aims and Objectives: Clinical manifestations and molecular and serological studies help determine the inflammatory response events associated with dengue severity. This study aimed to define the profiles of some cytokines and chemokines, as well as their molecular serotyping.
Materials and Methods: Blood serum samples are collected, followed by serodiagnosis (NS1 and IgM) through ELISA, molecular serotyping detection of dengue by real-time PCR through SyBr green, and an immunological study of cytokines tumor necrosis factor-alpha and interferon-gamma (IFN-γ) on mother-hand chemokines CCL26 and MCP1 by ELISA. Statistical analysis was performed by SPSS (version 22).
Results: The study examined 70 dengue seropositive samples, of which five were NS1 positive and 55 were IgM positive. After accounting for comorbidities and patients’ demographic classifications, the 21–30 age group has a maximum positivity of 30%. The mean concentration level of IFN-γ is elevated at 1544.438 pg/mL.
Conclusion: Dengue-positive patients have a high ischemic heart disease rate, among other diseases. An elevated circulating level of osteopetrosis may cause cancer, tuberculosis, and other serious diseases. In immunological aspects, IFN-γ elevation can be used to predict the severity of dengue infection. |
| first_indexed | 2024-04-09T14:58:12Z |
| format | Article |
| id | doaj.art-0f162518a8c04b5b9d967af67b2db46f |
| institution | Directory Open Access Journal |
| issn | 2467-9100 2091-0576 |
| language | English |
| last_indexed | 2024-04-09T14:58:12Z |
| publishDate | 2023-05-01 |
| publisher | Manipal College of Medical Sciences, Pokhara |
| record_format | Article |
| series | Asian Journal of Medical Sciences |
| spelling | doaj.art-0f162518a8c04b5b9d967af67b2db46f2023-05-01T23:55:00ZengManipal College of Medical Sciences, PokharaAsian Journal of Medical Sciences2467-91002091-05762023-05-01145121127https://doi.org/10.3126/ajms.v14i5.52001Clinicoepidemiological study of dengue positive patients admitted to tertiary care hospitals in the Burdwan district, using molecular seroprofiling and enzyme linked immunosorbent assays of cytokines and chemokinesNabamita Chaudhury0https://orcid.org/0000-0001-7929-6294Tanusri Biswas 1https://orcid.org/0000-0002-8599-5531Swati Nayek 2https://orcid.org/0000-0003-4272-9484Arghya Nath 3https://orcid.org/0000-0001-5498-1869Nivedita Mukherjee 4https://orcid.org/0000-0003-3417-495XSoumi Nag5https://orcid.org/0000-0002-7659-498XSuraj Mondal 6https://orcid.org/0000-0003-3467-3775Kuntal Das 7https://orcid.org/0000-0002-4082-881XAssistant Professor Co-P.I.-VRDL, Department of Microbiology, Burdwan Medical College and Hospital, Burdwan, West Bengal, IndiaAssociate Professor and Head, P.I.-VRDL, Department of Microbiology, Burdwan Medical College and Hospital, Burdwan, West Bengal, India Junior Resident, Department of Pathology, Burdwan Medical College and Hospital, Burdwan, West Bengal, India Research Scientist-B, ICMR-DHR Viral Research and Diagnostic Laboratory, Department of Microbiology, Burdwan Medical College, Burdwan, West Bengal, India Research Assistant, ICMR-DHR Viral Research and Diagnostic Laboratory, Department of Microbiology, Burdwan Medical College, Burdwan, West Bengal, India III Year, PGT, Department of Microbiology, Burdwan Medical College and Hospital, Burdwan, West Bengal, India Medical Lab Technologist, ICMR-DHR Viral Research and Diagnostic Laboratory, Department of Microbiology, Burdwan Medical College, Burdwan, West Bengal, India Medical Lab Technologist, ICMR-DHR Viral Research and Diagnostic Laboratory, Department of Microbiology, Burdwan Medical College, Burdwan, West Bengal, India Background: The World Health Organization views dengue fever as a significant worldwide public health threat in the tropics and subtropics. Dengue virus has four serotypes, DENG 1 to 4, which were responsible for outbreaks that occurred in other parts of the world from the 1980s until now. Aims and Objectives: Clinical manifestations and molecular and serological studies help determine the inflammatory response events associated with dengue severity. This study aimed to define the profiles of some cytokines and chemokines, as well as their molecular serotyping. Materials and Methods: Blood serum samples are collected, followed by serodiagnosis (NS1 and IgM) through ELISA, molecular serotyping detection of dengue by real-time PCR through SyBr green, and an immunological study of cytokines tumor necrosis factor-alpha and interferon-gamma (IFN-γ) on mother-hand chemokines CCL26 and MCP1 by ELISA. Statistical analysis was performed by SPSS (version 22). Results: The study examined 70 dengue seropositive samples, of which five were NS1 positive and 55 were IgM positive. After accounting for comorbidities and patients’ demographic classifications, the 21–30 age group has a maximum positivity of 30%. The mean concentration level of IFN-γ is elevated at 1544.438 pg/mL. Conclusion: Dengue-positive patients have a high ischemic heart disease rate, among other diseases. An elevated circulating level of osteopetrosis may cause cancer, tuberculosis, and other serious diseases. In immunological aspects, IFN-γ elevation can be used to predict the severity of dengue infection.https://www.nepjol.info/index.php/AJMS/article/view/52001dengue; elisa; real-time-pcr; serotype; deng-3; interferon-gamma; cytokines and chemokines; co-morbidites |
| spellingShingle | Nabamita Chaudhury Tanusri Biswas Swati Nayek Arghya Nath Nivedita Mukherjee Soumi Nag Suraj Mondal Kuntal Das Clinicoepidemiological study of dengue positive patients admitted to tertiary care hospitals in the Burdwan district, using molecular seroprofiling and enzyme linked immunosorbent assays of cytokines and chemokines Asian Journal of Medical Sciences dengue; elisa; real-time-pcr; serotype; deng-3; interferon-gamma; cytokines and chemokines; co-morbidites |
| title | Clinicoepidemiological study of dengue positive patients admitted to tertiary care hospitals in the Burdwan district, using molecular seroprofiling and enzyme linked immunosorbent assays of cytokines and chemokines |
| title_full | Clinicoepidemiological study of dengue positive patients admitted to tertiary care hospitals in the Burdwan district, using molecular seroprofiling and enzyme linked immunosorbent assays of cytokines and chemokines |
| title_fullStr | Clinicoepidemiological study of dengue positive patients admitted to tertiary care hospitals in the Burdwan district, using molecular seroprofiling and enzyme linked immunosorbent assays of cytokines and chemokines |
| title_full_unstemmed | Clinicoepidemiological study of dengue positive patients admitted to tertiary care hospitals in the Burdwan district, using molecular seroprofiling and enzyme linked immunosorbent assays of cytokines and chemokines |
| title_short | Clinicoepidemiological study of dengue positive patients admitted to tertiary care hospitals in the Burdwan district, using molecular seroprofiling and enzyme linked immunosorbent assays of cytokines and chemokines |
| title_sort | clinicoepidemiological study of dengue positive patients admitted to tertiary care hospitals in the burdwan district using molecular seroprofiling and enzyme linked immunosorbent assays of cytokines and chemokines |
| topic | dengue; elisa; real-time-pcr; serotype; deng-3; interferon-gamma; cytokines and chemokines; co-morbidites |
| url | https://www.nepjol.info/index.php/AJMS/article/view/52001 |
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