Repair of the abdominal wall with acellular bovine pericardial membranes - Part II - Histological and morphometric analyses
Introduction: Histological analysis is the main tool for evaluating acellular bioprostheses, mostly on an experimental basis. The objective is to histologically analyze the acellular matrix of bovine pericardium in abdominal wall repairs implanted in humans. Method: From a series of 30 r...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Cirurgia Plástica
2024-02-01
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| Series: | Revista Brasileira de Cirurgia Plástica |
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| Online Access: | http://www.rbcp.org.br/export-pdf/3408/1983-5175-rbcp-39-01-e0799-en.pdf |
| _version_ | 1827069385724919808 |
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| author | Luiz Fernando Frascino Luca Reis Frascino Jorge Alberto Thome Moacir Fernandes de Godoy |
| author_facet | Luiz Fernando Frascino Luca Reis Frascino Jorge Alberto Thome Moacir Fernandes de Godoy |
| author_sort | Luiz Fernando Frascino |
| collection | DOAJ |
| description | Introduction: Histological analysis is the main tool for evaluating acellular bioprostheses, mostly on an experimental basis. The objective is to histologically analyze the acellular matrix of bovine pericardium in abdominal wall repairs implanted in humans.
Method: From a series of 30 repairs with the membrane, 3 patients underwent surgical revision unrelated to the implants at 13, 22, and 23 months postoperatively, obtaining biopsies of the previously implanted areas. In addition to evaluating the basic aspects of biocompatibility and tissue neoformation, the slides were digitalized and subjected to computerized analysis with the ImageJ software to quantify the kinetics of membrane degradation associated with the analysis of the fractal dimension of the samples. The values obtained for percentages of residual membrane had their means compared by analysis of variance (ANOVA) and the unpaired Student’s T test, also used for the fractal dimension quantification values.
Results: The biocompatibility of the material was demonstrated, with tissue neoformation, collagen deposition, and cellularized tissue with a normal appearance without important local reactions. Residual fragments of the membrane were quantified at 40%±7% at 13 months, at 20%±6% at 22 months, and at 17%±6% at 23 months postoperatively, with the analysis of the fractal dimension indicating a progressive degradation of implants, with statistical significance between 13 months and late samples.
Conclusion: The results confirmed the functionality of the acellular bovine pericardium under different levels of mechanical stress in abdominal wall repairs in humans. |
| first_indexed | 2024-03-08T04:59:04Z |
| format | Article |
| id | doaj.art-0f185d2beea84a9789717d52336b03df |
| institution | Directory Open Access Journal |
| issn | 1983-5175 2177-1235 |
| language | English |
| last_indexed | 2025-03-20T00:05:47Z |
| publishDate | 2024-02-01 |
| publisher | Sociedade Brasileira de Cirurgia Plástica |
| record_format | Article |
| series | Revista Brasileira de Cirurgia Plástica |
| spelling | doaj.art-0f185d2beea84a9789717d52336b03df2024-10-11T18:15:27ZengSociedade Brasileira de Cirurgia PlásticaRevista Brasileira de Cirurgia Plástica1983-51752177-12352024-02-0139110.5935/2177-1235.2023RBCP0799-ENRepair of the abdominal wall with acellular bovine pericardial membranes - Part II - Histological and morphometric analysesLuiz Fernando Frascino0Luca Reis Frascino1Jorge Alberto Thome2Moacir Fernandes de Godoy3Hospital do Coração de São José do Rio Preto, São José do Rio Preto, SP, BrazilInstituto Frascino, Unidade de Publicação e Pesquisa, São José do Rio Preto, SP, BrazilFaculdade de Medicina de São José do Rio Preto, São José do Rio Preto, SP, BrazilFaculdade de Medicina de São José do Rio Preto, São José do Rio Preto, SP, BrazilIntroduction: Histological analysis is the main tool for evaluating acellular bioprostheses, mostly on an experimental basis. The objective is to histologically analyze the acellular matrix of bovine pericardium in abdominal wall repairs implanted in humans. Method: From a series of 30 repairs with the membrane, 3 patients underwent surgical revision unrelated to the implants at 13, 22, and 23 months postoperatively, obtaining biopsies of the previously implanted areas. In addition to evaluating the basic aspects of biocompatibility and tissue neoformation, the slides were digitalized and subjected to computerized analysis with the ImageJ software to quantify the kinetics of membrane degradation associated with the analysis of the fractal dimension of the samples. The values obtained for percentages of residual membrane had their means compared by analysis of variance (ANOVA) and the unpaired Student’s T test, also used for the fractal dimension quantification values. Results: The biocompatibility of the material was demonstrated, with tissue neoformation, collagen deposition, and cellularized tissue with a normal appearance without important local reactions. Residual fragments of the membrane were quantified at 40%±7% at 13 months, at 20%±6% at 22 months, and at 17%±6% at 23 months postoperatively, with the analysis of the fractal dimension indicating a progressive degradation of implants, with statistical significance between 13 months and late samples. Conclusion: The results confirmed the functionality of the acellular bovine pericardium under different levels of mechanical stress in abdominal wall repairs in humans.http://www.rbcp.org.br/export-pdf/3408/1983-5175-rbcp-39-01-e0799-en.pdfextracellular matrixabdominal herniaabdominal wallprosthetics and implantssurgical meshesbioprosthesispericardium |
| spellingShingle | Luiz Fernando Frascino Luca Reis Frascino Jorge Alberto Thome Moacir Fernandes de Godoy Repair of the abdominal wall with acellular bovine pericardial membranes - Part II - Histological and morphometric analyses Revista Brasileira de Cirurgia Plástica extracellular matrix abdominal hernia abdominal wall prosthetics and implants surgical meshes bioprosthesis pericardium |
| title | Repair of the abdominal wall with acellular bovine pericardial membranes - Part II - Histological and morphometric analyses |
| title_full | Repair of the abdominal wall with acellular bovine pericardial membranes - Part II - Histological and morphometric analyses |
| title_fullStr | Repair of the abdominal wall with acellular bovine pericardial membranes - Part II - Histological and morphometric analyses |
| title_full_unstemmed | Repair of the abdominal wall with acellular bovine pericardial membranes - Part II - Histological and morphometric analyses |
| title_short | Repair of the abdominal wall with acellular bovine pericardial membranes - Part II - Histological and morphometric analyses |
| title_sort | repair of the abdominal wall with acellular bovine pericardial membranes part ii histological and morphometric analyses |
| topic | extracellular matrix abdominal hernia abdominal wall prosthetics and implants surgical meshes bioprosthesis pericardium |
| url | http://www.rbcp.org.br/export-pdf/3408/1983-5175-rbcp-39-01-e0799-en.pdf |
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