Electron cryo-tomography provides insight into procentriole architecture and assembly mechanism
Centriole is an essential structure with multiple functions in cellular processes. Centriole biogenesis and homeostasis is tightly regulated. Using electron cryo-tomography (cryoET) we present the structure of procentrioles from Chlamydomonas reinhardtii. We identified a set of non-tubulin component...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2019-02-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/43434 |
| _version_ | 1811227822782414848 |
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| author | Sam Li Jose-Jesus Fernandez Wallace F Marshall David A Agard |
| author_facet | Sam Li Jose-Jesus Fernandez Wallace F Marshall David A Agard |
| author_sort | Sam Li |
| collection | DOAJ |
| description | Centriole is an essential structure with multiple functions in cellular processes. Centriole biogenesis and homeostasis is tightly regulated. Using electron cryo-tomography (cryoET) we present the structure of procentrioles from Chlamydomonas reinhardtii. We identified a set of non-tubulin components attached to the triplet microtubule (MT), many are at the junctions of tubules likely to reinforce the triplet. We describe structure of the A-C linker that bridges neighboring triplets. The structure infers that POC1 is likely an integral component of A-C linker. Its conserved WD40 β-propeller domain provides attachment sites for other A-C linker components. The twist of A-C linker results in an iris diaphragm-like motion of the triplets in the longitudinal direction of procentriole. Finally, we identified two assembly intermediates at the growing ends of procentriole allowing us to propose a model for the procentriole assembly. Our results provide a comprehensive structural framework for understanding the molecular mechanisms underpinning procentriole biogenesis and assembly. |
| first_indexed | 2024-04-12T09:48:24Z |
| format | Article |
| id | doaj.art-0f190cbfa5084a8cb923d8dc27809da8 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T09:48:24Z |
| publishDate | 2019-02-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-0f190cbfa5084a8cb923d8dc27809da82022-12-22T03:37:53ZengeLife Sciences Publications LtdeLife2050-084X2019-02-01810.7554/eLife.43434Electron cryo-tomography provides insight into procentriole architecture and assembly mechanismSam Li0https://orcid.org/0000-0002-0210-8192Jose-Jesus Fernandez1https://orcid.org/0000-0003-2222-3355Wallace F Marshall2https://orcid.org/0000-0002-8467-5763David A Agard3https://orcid.org/0000-0003-3512-695XDepartment of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, United StatesCentro Nacional de Biotecnologia (CSIC), Madrid, SpainDepartment of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, United StatesDepartment of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, United States; Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, United StatesCentriole is an essential structure with multiple functions in cellular processes. Centriole biogenesis and homeostasis is tightly regulated. Using electron cryo-tomography (cryoET) we present the structure of procentrioles from Chlamydomonas reinhardtii. We identified a set of non-tubulin components attached to the triplet microtubule (MT), many are at the junctions of tubules likely to reinforce the triplet. We describe structure of the A-C linker that bridges neighboring triplets. The structure infers that POC1 is likely an integral component of A-C linker. Its conserved WD40 β-propeller domain provides attachment sites for other A-C linker components. The twist of A-C linker results in an iris diaphragm-like motion of the triplets in the longitudinal direction of procentriole. Finally, we identified two assembly intermediates at the growing ends of procentriole allowing us to propose a model for the procentriole assembly. Our results provide a comprehensive structural framework for understanding the molecular mechanisms underpinning procentriole biogenesis and assembly.https://elifesciences.org/articles/43434centrioleprocentrioleelectron cryo-tomographysubtomogram averagingmicrotubule tripletstructure heterogeneity |
| spellingShingle | Sam Li Jose-Jesus Fernandez Wallace F Marshall David A Agard Electron cryo-tomography provides insight into procentriole architecture and assembly mechanism eLife centriole procentriole electron cryo-tomography subtomogram averaging microtubule triplet structure heterogeneity |
| title | Electron cryo-tomography provides insight into procentriole architecture and assembly mechanism |
| title_full | Electron cryo-tomography provides insight into procentriole architecture and assembly mechanism |
| title_fullStr | Electron cryo-tomography provides insight into procentriole architecture and assembly mechanism |
| title_full_unstemmed | Electron cryo-tomography provides insight into procentriole architecture and assembly mechanism |
| title_short | Electron cryo-tomography provides insight into procentriole architecture and assembly mechanism |
| title_sort | electron cryo tomography provides insight into procentriole architecture and assembly mechanism |
| topic | centriole procentriole electron cryo-tomography subtomogram averaging microtubule triplet structure heterogeneity |
| url | https://elifesciences.org/articles/43434 |
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