Cannabidiol Interacts Antagonistically with Cisplatin and Additively with Mitoxantrone in Various Melanoma Cell Lines—An Isobolographic Analysis
The medical application of cannabidiol (CBD) has been gathering increasing attention in recent years. This non-psychotropic cannabis-derived compound possesses antiepileptic, antipsychotic, anti-inflammatory and anxiolytic properties. Recent studies report that it also exerts antineoplastic effects...
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MDPI AG
2022-06-01
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| Series: | International Journal of Molecular Sciences |
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| author | Paweł Marzęda Paula Wróblewska-Łuczka Małgorzata Drozd Magdalena Florek-Łuszczki Katarzyna Załuska-Ogryzek Jarogniew J. Łuszczki |
| author_facet | Paweł Marzęda Paula Wróblewska-Łuczka Małgorzata Drozd Magdalena Florek-Łuszczki Katarzyna Załuska-Ogryzek Jarogniew J. Łuszczki |
| author_sort | Paweł Marzęda |
| collection | DOAJ |
| description | The medical application of cannabidiol (CBD) has been gathering increasing attention in recent years. This non-psychotropic cannabis-derived compound possesses antiepileptic, antipsychotic, anti-inflammatory and anxiolytic properties. Recent studies report that it also exerts antineoplastic effects in multiple types of cancers, including melanoma. In this in vitro study we tried to reveal the anticancer properties of CBD in malignant melanoma cell lines (SK-MEL 28, A375, FM55P and FM55M2) administered alone, as well as in combination with mitoxantrone (MTX) or cisplatin (CDDP). The effects of CBD on the viability of melanoma cells were measured by the MTT assay; cytotoxicity was determined in the LDH test and proliferation in the BrdU test. Moreover, the safety of CBD was tested in human keratinocytes (HaCaT) in LDH and MTT tests. Results indicate that CBD reduces the viability and proliferation of melanoma-malignant cells and exerts additive interactions with MTX. Unfortunately, CBD produced antagonistic interaction when combined with CDDP. CBD does not cause significant cytotoxicity in HaCaT cell line. In conclusion, CBD may be considered as a part of melanoma multi-drug therapy when combined with MTX. A special attention should be paid to the combination of CBD with CDDP due to the antagonistic interaction observed in the studied malignant melanoma cell lines. |
| first_indexed | 2024-03-09T23:32:47Z |
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| id | doaj.art-0f1c22e8488e49008ffcb4e832701d91 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T23:32:47Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-0f1c22e8488e49008ffcb4e832701d912023-11-23T17:06:12ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-06-012312675210.3390/ijms23126752Cannabidiol Interacts Antagonistically with Cisplatin and Additively with Mitoxantrone in Various Melanoma Cell Lines—An Isobolographic AnalysisPaweł Marzęda0Paula Wróblewska-Łuczka1Małgorzata Drozd2Magdalena Florek-Łuszczki3Katarzyna Załuska-Ogryzek4Jarogniew J. Łuszczki5Department of Pathophysiology, Medical University of Lublin, 20-090 Lublin, PolandDepartment of Pathophysiology, Medical University of Lublin, 20-090 Lublin, PolandDepartment of Pathophysiology, Medical University of Lublin, 20-090 Lublin, PolandDepartment of Medical Anthropology, Institute of Rural Health, 20-950 Lublin, PolandDepartment of Pathophysiology, Medical University of Lublin, 20-090 Lublin, PolandDepartment of Pathophysiology, Medical University of Lublin, 20-090 Lublin, PolandThe medical application of cannabidiol (CBD) has been gathering increasing attention in recent years. This non-psychotropic cannabis-derived compound possesses antiepileptic, antipsychotic, anti-inflammatory and anxiolytic properties. Recent studies report that it also exerts antineoplastic effects in multiple types of cancers, including melanoma. In this in vitro study we tried to reveal the anticancer properties of CBD in malignant melanoma cell lines (SK-MEL 28, A375, FM55P and FM55M2) administered alone, as well as in combination with mitoxantrone (MTX) or cisplatin (CDDP). The effects of CBD on the viability of melanoma cells were measured by the MTT assay; cytotoxicity was determined in the LDH test and proliferation in the BrdU test. Moreover, the safety of CBD was tested in human keratinocytes (HaCaT) in LDH and MTT tests. Results indicate that CBD reduces the viability and proliferation of melanoma-malignant cells and exerts additive interactions with MTX. Unfortunately, CBD produced antagonistic interaction when combined with CDDP. CBD does not cause significant cytotoxicity in HaCaT cell line. In conclusion, CBD may be considered as a part of melanoma multi-drug therapy when combined with MTX. A special attention should be paid to the combination of CBD with CDDP due to the antagonistic interaction observed in the studied malignant melanoma cell lines.https://www.mdpi.com/1422-0067/23/12/6752cannabinoidscannabidiolmelanomain vitro studydrug interactions |
| spellingShingle | Paweł Marzęda Paula Wróblewska-Łuczka Małgorzata Drozd Magdalena Florek-Łuszczki Katarzyna Załuska-Ogryzek Jarogniew J. Łuszczki Cannabidiol Interacts Antagonistically with Cisplatin and Additively with Mitoxantrone in Various Melanoma Cell Lines—An Isobolographic Analysis International Journal of Molecular Sciences cannabinoids cannabidiol melanoma in vitro study drug interactions |
| title | Cannabidiol Interacts Antagonistically with Cisplatin and Additively with Mitoxantrone in Various Melanoma Cell Lines—An Isobolographic Analysis |
| title_full | Cannabidiol Interacts Antagonistically with Cisplatin and Additively with Mitoxantrone in Various Melanoma Cell Lines—An Isobolographic Analysis |
| title_fullStr | Cannabidiol Interacts Antagonistically with Cisplatin and Additively with Mitoxantrone in Various Melanoma Cell Lines—An Isobolographic Analysis |
| title_full_unstemmed | Cannabidiol Interacts Antagonistically with Cisplatin and Additively with Mitoxantrone in Various Melanoma Cell Lines—An Isobolographic Analysis |
| title_short | Cannabidiol Interacts Antagonistically with Cisplatin and Additively with Mitoxantrone in Various Melanoma Cell Lines—An Isobolographic Analysis |
| title_sort | cannabidiol interacts antagonistically with cisplatin and additively with mitoxantrone in various melanoma cell lines an isobolographic analysis |
| topic | cannabinoids cannabidiol melanoma in vitro study drug interactions |
| url | https://www.mdpi.com/1422-0067/23/12/6752 |
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