Purification and characterization of a novel bacteriocin produced by Enterococcus faecalis CG-9 from human saliva
Strain CG-9, isolated from human saliva and identified as Enterococcus faecalis, produced a component that inhibits the growth of Gram-positive and Gram-negative bacteria. The active peptide from the cell-free supernatant of Enterococcus faecalis CG-9 was purified in three steps: (1) precipitation w...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Biotechnology & Biotechnological Equipment |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/13102818.2020.1830714 |
| _version_ | 1818562259226460160 |
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| author | Gongcheng Cui Changpei Pan Peng Xu Yanting Li Lijuan Wang Binting Gong Xiangping Li Shihai Huang |
| author_facet | Gongcheng Cui Changpei Pan Peng Xu Yanting Li Lijuan Wang Binting Gong Xiangping Li Shihai Huang |
| author_sort | Gongcheng Cui |
| collection | DOAJ |
| description | Strain CG-9, isolated from human saliva and identified as Enterococcus faecalis, produced a component that inhibits the growth of Gram-positive and Gram-negative bacteria. The active peptide from the cell-free supernatant of Enterococcus faecalis CG-9 was purified in three steps: (1) precipitation with 70% saturated ammonium sulfate: (2) cation-exchange chromatography, (3) gel filter chromatography. The purified bacteriocin exhibited a wide range of antimicrobial activity against many food-borne spoilage and Pathogenic bacteria such as Escherichia coli, Listeria monocytogenes, Staphylococcus aureus, as well as some yeast. The bacteriocin was heat stable, pH resistant and protease sensitive. Ethylenediaminetetraacetic acid, sodium dodecyl sulfate, Tween 80 and 6% sodium chloride did not decrease the activity. The characterization, antimicrobial spectrum and molecular mass of this bacteriocin suggested that it was a novel bacteriocin with potential research and application value in food industry. |
| first_indexed | 2024-12-14T01:01:21Z |
| format | Article |
| id | doaj.art-0f1d4f1c9e72474fa0ec1c6dd6f3fafa |
| institution | Directory Open Access Journal |
| issn | 1310-2818 1314-3530 |
| language | English |
| last_indexed | 2024-12-14T01:01:21Z |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj.art-0f1d4f1c9e72474fa0ec1c6dd6f3fafa2022-12-21T23:23:13ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302020-01-013411224123310.1080/13102818.2020.18307141830714Purification and characterization of a novel bacteriocin produced by Enterococcus faecalis CG-9 from human salivaGongcheng Cui0Changpei Pan1Peng Xu2Yanting Li3Lijuan Wang4Binting Gong5Xiangping Li6Shihai Huang7College of Life Science and Technology, Guangxi UniversityNanning Science and Technology Enterprise Incubation Base Co., LtdCollege of Life Science and Technology, Guangxi UniversityCollege of Life Science and Technology, Guangxi UniversityCollege of Life Science and Technology, Guangxi UniversityCollege of Life Science and Technology, Guangxi UniversityCollege of Animal Science and Technology, Guangxi UniversityCollege of Life Science and Technology, Guangxi UniversityStrain CG-9, isolated from human saliva and identified as Enterococcus faecalis, produced a component that inhibits the growth of Gram-positive and Gram-negative bacteria. The active peptide from the cell-free supernatant of Enterococcus faecalis CG-9 was purified in three steps: (1) precipitation with 70% saturated ammonium sulfate: (2) cation-exchange chromatography, (3) gel filter chromatography. The purified bacteriocin exhibited a wide range of antimicrobial activity against many food-borne spoilage and Pathogenic bacteria such as Escherichia coli, Listeria monocytogenes, Staphylococcus aureus, as well as some yeast. The bacteriocin was heat stable, pH resistant and protease sensitive. Ethylenediaminetetraacetic acid, sodium dodecyl sulfate, Tween 80 and 6% sodium chloride did not decrease the activity. The characterization, antimicrobial spectrum and molecular mass of this bacteriocin suggested that it was a novel bacteriocin with potential research and application value in food industry.http://dx.doi.org/10.1080/13102818.2020.1830714salivaenterococcus faecalisbacteriocinpurificationcharacteristics |
| spellingShingle | Gongcheng Cui Changpei Pan Peng Xu Yanting Li Lijuan Wang Binting Gong Xiangping Li Shihai Huang Purification and characterization of a novel bacteriocin produced by Enterococcus faecalis CG-9 from human saliva Biotechnology & Biotechnological Equipment saliva enterococcus faecalis bacteriocin purification characteristics |
| title | Purification and characterization of a novel bacteriocin produced by Enterococcus faecalis CG-9 from human saliva |
| title_full | Purification and characterization of a novel bacteriocin produced by Enterococcus faecalis CG-9 from human saliva |
| title_fullStr | Purification and characterization of a novel bacteriocin produced by Enterococcus faecalis CG-9 from human saliva |
| title_full_unstemmed | Purification and characterization of a novel bacteriocin produced by Enterococcus faecalis CG-9 from human saliva |
| title_short | Purification and characterization of a novel bacteriocin produced by Enterococcus faecalis CG-9 from human saliva |
| title_sort | purification and characterization of a novel bacteriocin produced by enterococcus faecalis cg 9 from human saliva |
| topic | saliva enterococcus faecalis bacteriocin purification characteristics |
| url | http://dx.doi.org/10.1080/13102818.2020.1830714 |
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