Cascading effects of polyphenol-rich purple corn pericarp extract on pupal, adult, and offspring of tobacco hornworm (Manduca sexta L.)
A major bottleneck in the commercialization of plant-based pest management compounds is that the extraction methods are complex, time-consuming, and even highly expensive. Using a recently developed inexpensive extraction and quantification methodology to isolate polyphenols (including anthocyanins...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Communicative & Integrative Biology |
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| Online Access: | http://dx.doi.org/10.1080/19420889.2020.1735223 |
| _version_ | 1819178379624054784 |
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| author | Mandeep Tayal Pavel Somavat Isabella Rodriguez Leilani Martinez Rupesh Kariyat |
| author_facet | Mandeep Tayal Pavel Somavat Isabella Rodriguez Leilani Martinez Rupesh Kariyat |
| author_sort | Mandeep Tayal |
| collection | DOAJ |
| description | A major bottleneck in the commercialization of plant-based pest management compounds is that the extraction methods are complex, time-consuming, and even highly expensive. Using a recently developed inexpensive extraction and quantification methodology to isolate polyphenols (including anthocyanins and condensed tannins) from purple corn pericarp, we examined their effects on Manduca sexta, a common insect herbivore. Following up on our previous work which demonstrated the negative impacts of polyphenol-rich extract on larval stages, we further examined whether there are any cascading effects on subsequent life stages (pupal and adult) including any possible transgenerational effects. Our results show that polyphenol-rich purple corn extract-fed caterpillars had significantly lower pupal mass and survival. Moreover, adult moths also had lower mass when eclosed from caterpillars reared on the extract diet. To test whether there were any transgenerational effects, we allowed male and female adults fed on purple corn extract diet and control diet to mate and lay eggs in a full factorial experiment. We found that purple corn extract-fed adult pair laid a lower number of eggs compared to other treatments. In addition, we also found that second instar M. sexta caterpillars hatched from eggs laid by any mating combination with at least one parent reared on purple corn extract gained significantly lower mass compared to caterpillars with both parents reared on the control diet. Taken together, our results show that there are cascading negative effects for feeding purple corn pericarp extract on pupal, adult, and second generation of M. sexta, reaffirming its potential application as a cost-effective and environmentally friendly pest deterrent. |
| first_indexed | 2024-12-22T21:41:37Z |
| format | Article |
| id | doaj.art-0f206c42522448af9c271044583d4e50 |
| institution | Directory Open Access Journal |
| issn | 1942-0889 |
| language | English |
| last_indexed | 2024-12-22T21:41:37Z |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Communicative & Integrative Biology |
| spelling | doaj.art-0f206c42522448af9c271044583d4e502022-12-21T18:11:36ZengTaylor & Francis GroupCommunicative & Integrative Biology1942-08892020-01-01131435310.1080/19420889.2020.17352231735223Cascading effects of polyphenol-rich purple corn pericarp extract on pupal, adult, and offspring of tobacco hornworm (Manduca sexta L.)Mandeep Tayal0Pavel Somavat1Isabella Rodriguez2Leilani Martinez3Rupesh Kariyat4The University of Texas Rio Grande ValleyThe University of Texas Rio Grande ValleyThe University of Texas Rio Grande ValleyWeslaco High SchoolThe University of Texas Rio Grande ValleyA major bottleneck in the commercialization of plant-based pest management compounds is that the extraction methods are complex, time-consuming, and even highly expensive. Using a recently developed inexpensive extraction and quantification methodology to isolate polyphenols (including anthocyanins and condensed tannins) from purple corn pericarp, we examined their effects on Manduca sexta, a common insect herbivore. Following up on our previous work which demonstrated the negative impacts of polyphenol-rich extract on larval stages, we further examined whether there are any cascading effects on subsequent life stages (pupal and adult) including any possible transgenerational effects. Our results show that polyphenol-rich purple corn extract-fed caterpillars had significantly lower pupal mass and survival. Moreover, adult moths also had lower mass when eclosed from caterpillars reared on the extract diet. To test whether there were any transgenerational effects, we allowed male and female adults fed on purple corn extract diet and control diet to mate and lay eggs in a full factorial experiment. We found that purple corn extract-fed adult pair laid a lower number of eggs compared to other treatments. In addition, we also found that second instar M. sexta caterpillars hatched from eggs laid by any mating combination with at least one parent reared on purple corn extract gained significantly lower mass compared to caterpillars with both parents reared on the control diet. Taken together, our results show that there are cascading negative effects for feeding purple corn pericarp extract on pupal, adult, and second generation of M. sexta, reaffirming its potential application as a cost-effective and environmentally friendly pest deterrent.http://dx.doi.org/10.1080/19420889.2020.1735223tobacco hornwormpolyphenolspurple cornherbivoregrowth and developmentpupal mass |
| spellingShingle | Mandeep Tayal Pavel Somavat Isabella Rodriguez Leilani Martinez Rupesh Kariyat Cascading effects of polyphenol-rich purple corn pericarp extract on pupal, adult, and offspring of tobacco hornworm (Manduca sexta L.) Communicative & Integrative Biology tobacco hornworm polyphenols purple corn herbivore growth and development pupal mass |
| title | Cascading effects of polyphenol-rich purple corn pericarp extract on pupal, adult, and offspring of tobacco hornworm (Manduca sexta L.) |
| title_full | Cascading effects of polyphenol-rich purple corn pericarp extract on pupal, adult, and offspring of tobacco hornworm (Manduca sexta L.) |
| title_fullStr | Cascading effects of polyphenol-rich purple corn pericarp extract on pupal, adult, and offspring of tobacco hornworm (Manduca sexta L.) |
| title_full_unstemmed | Cascading effects of polyphenol-rich purple corn pericarp extract on pupal, adult, and offspring of tobacco hornworm (Manduca sexta L.) |
| title_short | Cascading effects of polyphenol-rich purple corn pericarp extract on pupal, adult, and offspring of tobacco hornworm (Manduca sexta L.) |
| title_sort | cascading effects of polyphenol rich purple corn pericarp extract on pupal adult and offspring of tobacco hornworm manduca sexta l |
| topic | tobacco hornworm polyphenols purple corn herbivore growth and development pupal mass |
| url | http://dx.doi.org/10.1080/19420889.2020.1735223 |
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