RNA N<sup>6</sup>-Methyladenosine Affects Copper-Induced Oxidative Stress Response in <i>Arabidopsis thaliana</i>
Recently, post-transcriptional regulation of mRNA mediated by N<sup>6</sup>-methyladenosine (m<sup>6</sup>A) has been found to have profound effects on transcriptome regulation during plant responses to various abiotic stresses. However, whether this RNA modification can affe...
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MDPI AG
2024-01-01
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| Series: | Non-Coding RNA |
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| Online Access: | https://www.mdpi.com/2311-553X/10/1/8 |
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| author | Bishwas Sharma Ganesan Govindan Yongfang Li Ramanjulu Sunkar Brian D. Gregory |
| author_facet | Bishwas Sharma Ganesan Govindan Yongfang Li Ramanjulu Sunkar Brian D. Gregory |
| author_sort | Bishwas Sharma |
| collection | DOAJ |
| description | Recently, post-transcriptional regulation of mRNA mediated by N<sup>6</sup>-methyladenosine (m<sup>6</sup>A) has been found to have profound effects on transcriptome regulation during plant responses to various abiotic stresses. However, whether this RNA modification can affect an oxidative stress response in plants has not been studied. To assess the role of m<sup>6</sup>A modifications during copper-induced oxidative stress responses, m<sup>6</sup>A-IP-seq was performed in Arabidopsis seedlings exposed to high levels of copper sulfate. This analysis revealed large-scale shifts in this modification on the transcripts most relevant for oxidative stress. This altered epitranscriptomic mark is known to influence transcript abundance and translation; therefore we scrutinized these possibilities. We found an increased abundance of copper-enriched m<sup>6</sup>A-containing transcripts. Similarly, we also found increased ribosome occupancy of copper-enriched m<sup>6</sup>A-containing transcripts, specifically those encoding proteins involved with stress responses relevant to oxidative stressors. Furthermore, the significance of the m<sup>6</sup>A epitranscriptome on plant oxidative stress tolerance was uncovered by assessing germination and seedling development of the <i>mta</i> (N<sup>6</sup>-methyladenosine RNA methyltransferase A mutant complemented with <i>ABI3</i>:<i>MTA</i>) mutant exposed to high copper treatment. These analyses suggested hypersensitivity of the <i>mta</i> mutant compared to the wild-type plants in response to copper-induced oxidative stress. Overall, our findings suggest an important role for m<sup>6</sup>A in the oxidative stress response of Arabidopsis. |
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| last_indexed | 2024-03-07T22:18:59Z |
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| series | Non-Coding RNA |
| spelling | doaj.art-0f21ba3beb134674bff4f9bee69195ea2024-02-23T15:29:35ZengMDPI AGNon-Coding RNA2311-553X2024-01-01101810.3390/ncrna10010008RNA N<sup>6</sup>-Methyladenosine Affects Copper-Induced Oxidative Stress Response in <i>Arabidopsis thaliana</i>Bishwas Sharma0Ganesan Govindan1Yongfang Li2Ramanjulu Sunkar3Brian D. Gregory4Department of Biology, University of Pennsylvania, Philadelphia, PA 19104, USADepartment of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078, USADepartment of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078, USADepartment of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK 74078, USADepartment of Biology, University of Pennsylvania, Philadelphia, PA 19104, USARecently, post-transcriptional regulation of mRNA mediated by N<sup>6</sup>-methyladenosine (m<sup>6</sup>A) has been found to have profound effects on transcriptome regulation during plant responses to various abiotic stresses. However, whether this RNA modification can affect an oxidative stress response in plants has not been studied. To assess the role of m<sup>6</sup>A modifications during copper-induced oxidative stress responses, m<sup>6</sup>A-IP-seq was performed in Arabidopsis seedlings exposed to high levels of copper sulfate. This analysis revealed large-scale shifts in this modification on the transcripts most relevant for oxidative stress. This altered epitranscriptomic mark is known to influence transcript abundance and translation; therefore we scrutinized these possibilities. We found an increased abundance of copper-enriched m<sup>6</sup>A-containing transcripts. Similarly, we also found increased ribosome occupancy of copper-enriched m<sup>6</sup>A-containing transcripts, specifically those encoding proteins involved with stress responses relevant to oxidative stressors. Furthermore, the significance of the m<sup>6</sup>A epitranscriptome on plant oxidative stress tolerance was uncovered by assessing germination and seedling development of the <i>mta</i> (N<sup>6</sup>-methyladenosine RNA methyltransferase A mutant complemented with <i>ABI3</i>:<i>MTA</i>) mutant exposed to high copper treatment. These analyses suggested hypersensitivity of the <i>mta</i> mutant compared to the wild-type plants in response to copper-induced oxidative stress. Overall, our findings suggest an important role for m<sup>6</sup>A in the oxidative stress response of Arabidopsis.https://www.mdpi.com/2311-553X/10/1/8epitranscriptomelong non-coding RNAsplant stress responseRNA methylationribosome occupancytranscript stability |
| spellingShingle | Bishwas Sharma Ganesan Govindan Yongfang Li Ramanjulu Sunkar Brian D. Gregory RNA N<sup>6</sup>-Methyladenosine Affects Copper-Induced Oxidative Stress Response in <i>Arabidopsis thaliana</i> Non-Coding RNA epitranscriptome long non-coding RNAs plant stress response RNA methylation ribosome occupancy transcript stability |
| title | RNA N<sup>6</sup>-Methyladenosine Affects Copper-Induced Oxidative Stress Response in <i>Arabidopsis thaliana</i> |
| title_full | RNA N<sup>6</sup>-Methyladenosine Affects Copper-Induced Oxidative Stress Response in <i>Arabidopsis thaliana</i> |
| title_fullStr | RNA N<sup>6</sup>-Methyladenosine Affects Copper-Induced Oxidative Stress Response in <i>Arabidopsis thaliana</i> |
| title_full_unstemmed | RNA N<sup>6</sup>-Methyladenosine Affects Copper-Induced Oxidative Stress Response in <i>Arabidopsis thaliana</i> |
| title_short | RNA N<sup>6</sup>-Methyladenosine Affects Copper-Induced Oxidative Stress Response in <i>Arabidopsis thaliana</i> |
| title_sort | rna n sup 6 sup methyladenosine affects copper induced oxidative stress response in i arabidopsis thaliana i |
| topic | epitranscriptome long non-coding RNAs plant stress response RNA methylation ribosome occupancy transcript stability |
| url | https://www.mdpi.com/2311-553X/10/1/8 |
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