Autologous Chondrocyte Transplantation in the Repair of Full-Thickness Focal Cartilage Damage in Rabbits
Purpose. To compare the efficacy of autologous chondrocyte transplantation (ACT) versus non-operative measures for cartilage repair in rabbits. Methods. Nine New Zealand white rabbits were used. Identical focal defects were created in the articular cartilage of both knees. One month later, the right...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2008-08-01
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| Series: | Journal of Orthopaedic Surgery |
| Online Access: | https://doi.org/10.1177/230949900801600220 |
| _version_ | 1818160069290754048 |
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| author | T Kamarul L Selvaratnam T Masjuddin S Ab-Rahim C Ng KY Chan TS Ahmad |
| author_facet | T Kamarul L Selvaratnam T Masjuddin S Ab-Rahim C Ng KY Chan TS Ahmad |
| author_sort | T Kamarul |
| collection | DOAJ |
| description | Purpose. To compare the efficacy of autologous chondrocyte transplantation (ACT) versus non-operative measures for cartilage repair in rabbits. Methods. Nine New Zealand white rabbits were used. Identical focal defects were created in the articular cartilage of both knees. One month later, the right knee was repaired via ACT, while the left knee was left untreated (control group). The quality of cartilage tissues in both knees was compared 3 months later, according to the quantitative analysis of glycosaminoglycan (GAG) in the cartilage and macroscopic examination of histology using the Brittberg/International Cartilage Research Society (ICRS) score. Results. Microscopic examination showed enhanced regeneration following ACT repair. Quantification analysis revealed significantly higher cellular expression of GAG in the ACT-treated knees (1.12 vs 0.81 μg GAGs/mg protein, p=0.008). The mean Brittberg/ICRS score was significantly higher in the treated knees (6.00 vs 1.89, p=0.007). Conclusion. ACT is superior to non-operative measures for repairing focal cartilage defects, as determined by favourable histological and immunohistological outcomes at the cellular level. |
| first_indexed | 2024-12-11T15:56:01Z |
| format | Article |
| id | doaj.art-0f22c66ffc1649899c9b10bf11be6be2 |
| institution | Directory Open Access Journal |
| issn | 2309-4990 |
| language | English |
| last_indexed | 2024-12-11T15:56:01Z |
| publishDate | 2008-08-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of Orthopaedic Surgery |
| spelling | doaj.art-0f22c66ffc1649899c9b10bf11be6be22022-12-22T00:59:25ZengSAGE PublishingJournal of Orthopaedic Surgery2309-49902008-08-011610.1177/230949900801600220Autologous Chondrocyte Transplantation in the Repair of Full-Thickness Focal Cartilage Damage in RabbitsT KamarulL Selvaratnam0T MasjuddinS Ab-RahimC NgKY Chan1TS Ahmad Current affiliation: Monash University, Sunway Campus, Selangor, Malaysia Current affiliation: Gleneagles Hospital, Kuala Lumpur, MalaysiaPurpose. To compare the efficacy of autologous chondrocyte transplantation (ACT) versus non-operative measures for cartilage repair in rabbits. Methods. Nine New Zealand white rabbits were used. Identical focal defects were created in the articular cartilage of both knees. One month later, the right knee was repaired via ACT, while the left knee was left untreated (control group). The quality of cartilage tissues in both knees was compared 3 months later, according to the quantitative analysis of glycosaminoglycan (GAG) in the cartilage and macroscopic examination of histology using the Brittberg/International Cartilage Research Society (ICRS) score. Results. Microscopic examination showed enhanced regeneration following ACT repair. Quantification analysis revealed significantly higher cellular expression of GAG in the ACT-treated knees (1.12 vs 0.81 μg GAGs/mg protein, p=0.008). The mean Brittberg/ICRS score was significantly higher in the treated knees (6.00 vs 1.89, p=0.007). Conclusion. ACT is superior to non-operative measures for repairing focal cartilage defects, as determined by favourable histological and immunohistological outcomes at the cellular level.https://doi.org/10.1177/230949900801600220 |
| spellingShingle | T Kamarul L Selvaratnam T Masjuddin S Ab-Rahim C Ng KY Chan TS Ahmad Autologous Chondrocyte Transplantation in the Repair of Full-Thickness Focal Cartilage Damage in Rabbits Journal of Orthopaedic Surgery |
| title | Autologous Chondrocyte Transplantation in the Repair of Full-Thickness Focal Cartilage Damage in Rabbits |
| title_full | Autologous Chondrocyte Transplantation in the Repair of Full-Thickness Focal Cartilage Damage in Rabbits |
| title_fullStr | Autologous Chondrocyte Transplantation in the Repair of Full-Thickness Focal Cartilage Damage in Rabbits |
| title_full_unstemmed | Autologous Chondrocyte Transplantation in the Repair of Full-Thickness Focal Cartilage Damage in Rabbits |
| title_short | Autologous Chondrocyte Transplantation in the Repair of Full-Thickness Focal Cartilage Damage in Rabbits |
| title_sort | autologous chondrocyte transplantation in the repair of full thickness focal cartilage damage in rabbits |
| url | https://doi.org/10.1177/230949900801600220 |
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