Elongation factor P controls translation of the mgtA gene encoding a Mg2+ transporter during Salmonella infection
Abstract Ribosome often stalls on mRNA sequences harboring consecutive proline codons. Elongation factor P (EF‐P) is required for the stalled ribosome to continue translation and thus the absence of EF‐P affects translation of the associated open reading frame. Here we report that EF‐P controls tran...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-04-01
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| Series: | MicrobiologyOpen |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/mbo3.680 |
| _version_ | 1811212333079330816 |
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| author | Eunna Choi Daesil Nam Jeongjoon Choi Shinae Park Jung‐Shin Lee Eun‐Jin Lee |
| author_facet | Eunna Choi Daesil Nam Jeongjoon Choi Shinae Park Jung‐Shin Lee Eun‐Jin Lee |
| author_sort | Eunna Choi |
| collection | DOAJ |
| description | Abstract Ribosome often stalls on mRNA sequences harboring consecutive proline codons. Elongation factor P (EF‐P) is required for the stalled ribosome to continue translation and thus the absence of EF‐P affects translation of the associated open reading frame. Here we report that EF‐P controls translation of the mgtA gene encoding a Mg2+‐transporting ATPase from the intracellualr pathogen Salmonella enterica serovar Typhimurium. EF‐P's effect on mgtA translation is dependent on the 550th and 551st proline codons in the coding region and thus substitution of those proline codons eliminates EF‐P‐mediated control of MgtA protein without affecting the Mg2+‐transporting activity of the mgtA gene. The Pro550 and Pro551‐substituted mgtA gene promotes Salmonella's intramacrophage survival and mouse virulence, suggesting that EF‐P‐mediated translational control of the mgtA gene is required for Salmonella pathogenesis. |
| first_indexed | 2024-04-12T05:27:35Z |
| format | Article |
| id | doaj.art-0f2325909e8f4803b3231f923cc964d4 |
| institution | Directory Open Access Journal |
| issn | 2045-8827 |
| language | English |
| last_indexed | 2024-04-12T05:27:35Z |
| publishDate | 2019-04-01 |
| publisher | Wiley |
| record_format | Article |
| series | MicrobiologyOpen |
| spelling | doaj.art-0f2325909e8f4803b3231f923cc964d42022-12-22T03:46:12ZengWileyMicrobiologyOpen2045-88272019-04-0184n/an/a10.1002/mbo3.680Elongation factor P controls translation of the mgtA gene encoding a Mg2+ transporter during Salmonella infectionEunna Choi0Daesil Nam1Jeongjoon Choi2Shinae Park3Jung‐Shin Lee4Eun‐Jin Lee5Department of Genetic Engineering and Graduate School of Biotechnology College of Life Sciences Kyung Hee University Yongin South KoreaDivision of Microbiology Department of Molecular Cell Biology Samsung Biomedical Research Institute Sungkyunkwan University School of Medicine Suwon South KoreaDepartment of Microbial Pathogenesis Yale School of Medicine New Haven ConnecticutDepartment of Molecular Bioscience College of Biomedical Science Kangwon National University Chuncheon South KoreaDepartment of Molecular Bioscience College of Biomedical Science Kangwon National University Chuncheon South KoreaDepartment of Genetic Engineering and Graduate School of Biotechnology College of Life Sciences Kyung Hee University Yongin South KoreaAbstract Ribosome often stalls on mRNA sequences harboring consecutive proline codons. Elongation factor P (EF‐P) is required for the stalled ribosome to continue translation and thus the absence of EF‐P affects translation of the associated open reading frame. Here we report that EF‐P controls translation of the mgtA gene encoding a Mg2+‐transporting ATPase from the intracellualr pathogen Salmonella enterica serovar Typhimurium. EF‐P's effect on mgtA translation is dependent on the 550th and 551st proline codons in the coding region and thus substitution of those proline codons eliminates EF‐P‐mediated control of MgtA protein without affecting the Mg2+‐transporting activity of the mgtA gene. The Pro550 and Pro551‐substituted mgtA gene promotes Salmonella's intramacrophage survival and mouse virulence, suggesting that EF‐P‐mediated translational control of the mgtA gene is required for Salmonella pathogenesis.https://doi.org/10.1002/mbo3.680elongation factor PMg2+ transportermgtAtranslational control |
| spellingShingle | Eunna Choi Daesil Nam Jeongjoon Choi Shinae Park Jung‐Shin Lee Eun‐Jin Lee Elongation factor P controls translation of the mgtA gene encoding a Mg2+ transporter during Salmonella infection MicrobiologyOpen elongation factor P Mg2+ transporter mgtA translational control |
| title | Elongation factor P controls translation of the mgtA gene encoding a Mg2+ transporter during Salmonella infection |
| title_full | Elongation factor P controls translation of the mgtA gene encoding a Mg2+ transporter during Salmonella infection |
| title_fullStr | Elongation factor P controls translation of the mgtA gene encoding a Mg2+ transporter during Salmonella infection |
| title_full_unstemmed | Elongation factor P controls translation of the mgtA gene encoding a Mg2+ transporter during Salmonella infection |
| title_short | Elongation factor P controls translation of the mgtA gene encoding a Mg2+ transporter during Salmonella infection |
| title_sort | elongation factor p controls translation of the mgta gene encoding a mg2 transporter during salmonella infection |
| topic | elongation factor P Mg2+ transporter mgtA translational control |
| url | https://doi.org/10.1002/mbo3.680 |
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