An Ultrafast One-Step Quantitative Reverse Transcription–Polymerase Chain Reaction Assay for Detection of SARS-CoV-2
We developed an ultrafast one-step RT-qPCR assay for SARS-CoV-2 detection, which can be completed in only 30 min on benchtop Bio-Rad CFX96. The assay significantly reduces the running time of conventional RT-qPCR: reduced RT step from 10 to 1 min, and reduced the PCR cycle of denaturation from 10 to...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.749783/full |
| _version_ | 1818568664716148736 |
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| author | Jadranka Milosevic Jadranka Milosevic Mengrou Lu Wallace Greene Hong-Zhang He Hong-Zhang He Si-Yang Zheng Si-Yang Zheng |
| author_facet | Jadranka Milosevic Jadranka Milosevic Mengrou Lu Wallace Greene Hong-Zhang He Hong-Zhang He Si-Yang Zheng Si-Yang Zheng |
| author_sort | Jadranka Milosevic |
| collection | DOAJ |
| description | We developed an ultrafast one-step RT-qPCR assay for SARS-CoV-2 detection, which can be completed in only 30 min on benchtop Bio-Rad CFX96. The assay significantly reduces the running time of conventional RT-qPCR: reduced RT step from 10 to 1 min, and reduced the PCR cycle of denaturation from 10 to 1 s and extension from 30 to 1 s. A cohort of 60 nasopharyngeal swab samples testing showed that the assay had a clinical sensitivity of 100% and a clinical specificity of 100%. |
| first_indexed | 2024-12-14T06:38:13Z |
| format | Article |
| id | doaj.art-0f23aaf52ea64b4ba3ecd0d4c47ef909 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-14T06:38:13Z |
| publishDate | 2021-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-0f23aaf52ea64b4ba3ecd0d4c47ef9092022-12-21T23:13:18ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-11-011210.3389/fmicb.2021.749783749783An Ultrafast One-Step Quantitative Reverse Transcription–Polymerase Chain Reaction Assay for Detection of SARS-CoV-2Jadranka Milosevic0Jadranka Milosevic1Mengrou Lu2Wallace Greene3Hong-Zhang He4Hong-Zhang He5Si-Yang Zheng6Si-Yang Zheng7Captis Diagnostics Inc., Pittsburgh, PA, United StatesBiomedical Engineering Department, Carnegie Mellon University, Pittsburgh, PA, United StatesBiomedical Engineering Department, Carnegie Mellon University, Pittsburgh, PA, United StatesPenn State Hershey Medical Center, Penn State College of Medicine, Hershey, PA, United StatesCaptis Diagnostics Inc., Pittsburgh, PA, United StatesBiomedical Engineering Department, Carnegie Mellon University, Pittsburgh, PA, United StatesBiomedical Engineering Department, Carnegie Mellon University, Pittsburgh, PA, United StatesElectrical & Computer Engineering, Carnegie Mellon University, Pittsburgh, PA, United StatesWe developed an ultrafast one-step RT-qPCR assay for SARS-CoV-2 detection, which can be completed in only 30 min on benchtop Bio-Rad CFX96. The assay significantly reduces the running time of conventional RT-qPCR: reduced RT step from 10 to 1 min, and reduced the PCR cycle of denaturation from 10 to 1 s and extension from 30 to 1 s. A cohort of 60 nasopharyngeal swab samples testing showed that the assay had a clinical sensitivity of 100% and a clinical specificity of 100%.https://www.frontiersin.org/articles/10.3389/fmicb.2021.749783/fullultrafastone-step RT-qPCR assaySARS-CoV-2 detectionCOVID-19nasopharyngeal swab |
| spellingShingle | Jadranka Milosevic Jadranka Milosevic Mengrou Lu Wallace Greene Hong-Zhang He Hong-Zhang He Si-Yang Zheng Si-Yang Zheng An Ultrafast One-Step Quantitative Reverse Transcription–Polymerase Chain Reaction Assay for Detection of SARS-CoV-2 Frontiers in Microbiology ultrafast one-step RT-qPCR assay SARS-CoV-2 detection COVID-19 nasopharyngeal swab |
| title | An Ultrafast One-Step Quantitative Reverse Transcription–Polymerase Chain Reaction Assay for Detection of SARS-CoV-2 |
| title_full | An Ultrafast One-Step Quantitative Reverse Transcription–Polymerase Chain Reaction Assay for Detection of SARS-CoV-2 |
| title_fullStr | An Ultrafast One-Step Quantitative Reverse Transcription–Polymerase Chain Reaction Assay for Detection of SARS-CoV-2 |
| title_full_unstemmed | An Ultrafast One-Step Quantitative Reverse Transcription–Polymerase Chain Reaction Assay for Detection of SARS-CoV-2 |
| title_short | An Ultrafast One-Step Quantitative Reverse Transcription–Polymerase Chain Reaction Assay for Detection of SARS-CoV-2 |
| title_sort | ultrafast one step quantitative reverse transcription polymerase chain reaction assay for detection of sars cov 2 |
| topic | ultrafast one-step RT-qPCR assay SARS-CoV-2 detection COVID-19 nasopharyngeal swab |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.749783/full |
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