An Ultrafast One-Step Quantitative Reverse Transcription–Polymerase Chain Reaction Assay for Detection of SARS-CoV-2

An Ultrafast One-Step Quantitative Reverse Transcription–Polymerase Chain Reaction Assay for Detection of SARS-CoV-2

We developed an ultrafast one-step RT-qPCR assay for SARS-CoV-2 detection, which can be completed in only 30 min on benchtop Bio-Rad CFX96. The assay significantly reduces the running time of conventional RT-qPCR: reduced RT step from 10 to 1 min, and reduced the PCR cycle of denaturation from 10 to...

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Bibliographic Details
Main Authors: Jadranka Milosevic, Mengrou Lu, Wallace Greene, Hong-Zhang He, Si-Yang Zheng
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-11-01
Series:Frontiers in Microbiology
Subjects:
ultrafast
one-step RT-qPCR assay
SARS-CoV-2 detection
COVID-19
nasopharyngeal swab
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2021.749783/full

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