Pathogenesis-Related Genes of PR1, PR2, PR4, and PR5 Families Are Involved in the Response to <i>Fusarium</i> Infection in Garlic (<i>Allium sativum</i> L.)
Plants of the genus <i>Allium</i> developed a diversity of defense mechanisms against pathogenic fungi of the genus <i>Fusarium</i>, including transcriptional activation of pathogenesis-related (PR) genes. However, the information on the regulation of PR factors in garlic (&l...
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MDPI AG
2021-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/13/6688 |
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| author | Olga K. Anisimova Anna V. Shchennikova Elena Z. Kochieva Mikhail A. Filyushin |
| author_facet | Olga K. Anisimova Anna V. Shchennikova Elena Z. Kochieva Mikhail A. Filyushin |
| author_sort | Olga K. Anisimova |
| collection | DOAJ |
| description | Plants of the genus <i>Allium</i> developed a diversity of defense mechanisms against pathogenic fungi of the genus <i>Fusarium</i>, including transcriptional activation of pathogenesis-related (PR) genes. However, the information on the regulation of PR factors in garlic (<i>Allium sativum</i> L.) is limited. In the present study, we identified <i>AsPR</i> genes putatively encoding PR1, PR2, PR4, and PR5 proteins in <i>A. sativum</i> cv. Ershuizao, which may be involved in the defense against <i>Fusarium</i> infection. The promoters of the <i>AsPR1–5</i> genes contained jasmonic acid-, salicylic acid-, gibberellin-, abscisic acid-, auxin-, ethylene-, and stress-responsive elements associated with the response to plant parasites. The expression of <i>AsPR1c</i>, <i>d</i>, <i>g</i>, <i>k, AsPR2b</i>, <i>AsPR5a, c</i> (in roots), and <i>AsPR4a(c)</i>, <i>b,</i> and <i>AsPR2c</i> (in stems and cloves) significantly differed between garlic cultivars resistant and susceptible to <i>Fusarium</i> rot, suggesting that it could define the PR protein-mediated protection against <i>Fusarium</i> infection in garlic. Our results provide insights into the role of PR factors in <i>A. sativum</i> and may be useful for breeding programs to increase the resistance of <i>Allium</i> crops to <i>Fusarium</i> infections. |
| first_indexed | 2024-03-10T10:10:21Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T10:10:21Z |
| publishDate | 2021-06-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-0f256f93cceb4ff3aa9eed7f7a3906ec2023-11-22T01:13:24ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-06-012213668810.3390/ijms22136688Pathogenesis-Related Genes of PR1, PR2, PR4, and PR5 Families Are Involved in the Response to <i>Fusarium</i> Infection in Garlic (<i>Allium sativum</i> L.)Olga K. Anisimova0Anna V. Shchennikova1Elena Z. Kochieva2Mikhail A. Filyushin3Federal Research Centre “Fundamentals of Biotechnology” of the Russian Academy of Sciences, 119071 Moscow, RussiaFederal Research Centre “Fundamentals of Biotechnology” of the Russian Academy of Sciences, 119071 Moscow, RussiaFederal Research Centre “Fundamentals of Biotechnology” of the Russian Academy of Sciences, 119071 Moscow, RussiaFederal Research Centre “Fundamentals of Biotechnology” of the Russian Academy of Sciences, 119071 Moscow, RussiaPlants of the genus <i>Allium</i> developed a diversity of defense mechanisms against pathogenic fungi of the genus <i>Fusarium</i>, including transcriptional activation of pathogenesis-related (PR) genes. However, the information on the regulation of PR factors in garlic (<i>Allium sativum</i> L.) is limited. In the present study, we identified <i>AsPR</i> genes putatively encoding PR1, PR2, PR4, and PR5 proteins in <i>A. sativum</i> cv. Ershuizao, which may be involved in the defense against <i>Fusarium</i> infection. The promoters of the <i>AsPR1–5</i> genes contained jasmonic acid-, salicylic acid-, gibberellin-, abscisic acid-, auxin-, ethylene-, and stress-responsive elements associated with the response to plant parasites. The expression of <i>AsPR1c</i>, <i>d</i>, <i>g</i>, <i>k, AsPR2b</i>, <i>AsPR5a, c</i> (in roots), and <i>AsPR4a(c)</i>, <i>b,</i> and <i>AsPR2c</i> (in stems and cloves) significantly differed between garlic cultivars resistant and susceptible to <i>Fusarium</i> rot, suggesting that it could define the PR protein-mediated protection against <i>Fusarium</i> infection in garlic. Our results provide insights into the role of PR factors in <i>A. sativum</i> and may be useful for breeding programs to increase the resistance of <i>Allium</i> crops to <i>Fusarium</i> infections.https://www.mdpi.com/1422-0067/22/13/6688garlic <i>Allium sativum</i> L.pathogenesis-related proteinsbiotic stress<i>Fusarium</i> spp.gene structuregene expression |
| spellingShingle | Olga K. Anisimova Anna V. Shchennikova Elena Z. Kochieva Mikhail A. Filyushin Pathogenesis-Related Genes of PR1, PR2, PR4, and PR5 Families Are Involved in the Response to <i>Fusarium</i> Infection in Garlic (<i>Allium sativum</i> L.) International Journal of Molecular Sciences garlic <i>Allium sativum</i> L. pathogenesis-related proteins biotic stress <i>Fusarium</i> spp. gene structure gene expression |
| title | Pathogenesis-Related Genes of PR1, PR2, PR4, and PR5 Families Are Involved in the Response to <i>Fusarium</i> Infection in Garlic (<i>Allium sativum</i> L.) |
| title_full | Pathogenesis-Related Genes of PR1, PR2, PR4, and PR5 Families Are Involved in the Response to <i>Fusarium</i> Infection in Garlic (<i>Allium sativum</i> L.) |
| title_fullStr | Pathogenesis-Related Genes of PR1, PR2, PR4, and PR5 Families Are Involved in the Response to <i>Fusarium</i> Infection in Garlic (<i>Allium sativum</i> L.) |
| title_full_unstemmed | Pathogenesis-Related Genes of PR1, PR2, PR4, and PR5 Families Are Involved in the Response to <i>Fusarium</i> Infection in Garlic (<i>Allium sativum</i> L.) |
| title_short | Pathogenesis-Related Genes of PR1, PR2, PR4, and PR5 Families Are Involved in the Response to <i>Fusarium</i> Infection in Garlic (<i>Allium sativum</i> L.) |
| title_sort | pathogenesis related genes of pr1 pr2 pr4 and pr5 families are involved in the response to i fusarium i infection in garlic i allium sativum i l |
| topic | garlic <i>Allium sativum</i> L. pathogenesis-related proteins biotic stress <i>Fusarium</i> spp. gene structure gene expression |
| url | https://www.mdpi.com/1422-0067/22/13/6688 |
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