ThHSFA1 Confers Salt Stress Tolerance through Modulation of Reactive Oxygen Species Scavenging by Directly Regulating <i>ThWRKY4</i>
Heat shock transcription factors (HSFs) play critical roles in several types of environmental stresses. However, the detailed regulatory mechanisms in response to salt stress are still largely unknown. In this study, we examined the salt-induced transcriptional responses of <i>ThHSFA1</i>...
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MDPI AG
2021-05-01
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| Series: | International Journal of Molecular Sciences |
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| author | Ting-Ting Sun Chao Wang Rui Liu Yu Zhang Yu-Cheng Wang Liu-Qiang Wang |
| author_facet | Ting-Ting Sun Chao Wang Rui Liu Yu Zhang Yu-Cheng Wang Liu-Qiang Wang |
| author_sort | Ting-Ting Sun |
| collection | DOAJ |
| description | Heat shock transcription factors (HSFs) play critical roles in several types of environmental stresses. However, the detailed regulatory mechanisms in response to salt stress are still largely unknown. In this study, we examined the salt-induced transcriptional responses of <i>ThHSFA1</i>-<i>ThWRKY4</i> in <i>Tamarix</i> <i>hispida</i> and their functions and regulatory mechanisms in salt tolerance. ThHSFA1 protein acts as an upstream regulator that can directly activate <i>ThWRKY4</i> expression by binding to the heat shock element (HSE) of the <i>ThWRKY4</i> promoter using yeast one-hybrid (Y1H), chromatin immunoprecipitation (ChIP), and dual-luciferase reporter assays. <i>ThHSFA1</i> and <i>ThWRKY4</i> expression was significantly induced by salt stress and abscisic acid (ABA) treatment in the roots and leaves of <i>T</i>. <i>hispida</i>. ThHSFA1 is a nuclear-localized protein with transactivation activity at the <i>C</i>-terminus. Compared to nontransgenic plants, transgenic plants overexpressing <i>ThHSFA1</i> displayed enhanced salt tolerance and exhibited reduced reactive oxygen species (ROS) levels and increased antioxidant enzyme activity levels under salt stress. Therefore, we further concluded that <i>ThHSFA1</i> mediated the regulation of <i>ThWRKY4</i> in response to salt stress in <i>T</i>. <i>hispida</i>. |
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| spelling | doaj.art-0f27ece554504e1f931490fadd5714762023-11-21T19:02:02ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-05-01229504810.3390/ijms22095048ThHSFA1 Confers Salt Stress Tolerance through Modulation of Reactive Oxygen Species Scavenging by Directly Regulating <i>ThWRKY4</i>Ting-Ting Sun0Chao Wang1Rui Liu2Yu Zhang3Yu-Cheng Wang4Liu-Qiang Wang5State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of the State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, ChinaState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, ChinaState Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of the State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, ChinaState Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of the State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, ChinaState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, ChinaState Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of the State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, ChinaHeat shock transcription factors (HSFs) play critical roles in several types of environmental stresses. However, the detailed regulatory mechanisms in response to salt stress are still largely unknown. In this study, we examined the salt-induced transcriptional responses of <i>ThHSFA1</i>-<i>ThWRKY4</i> in <i>Tamarix</i> <i>hispida</i> and their functions and regulatory mechanisms in salt tolerance. ThHSFA1 protein acts as an upstream regulator that can directly activate <i>ThWRKY4</i> expression by binding to the heat shock element (HSE) of the <i>ThWRKY4</i> promoter using yeast one-hybrid (Y1H), chromatin immunoprecipitation (ChIP), and dual-luciferase reporter assays. <i>ThHSFA1</i> and <i>ThWRKY4</i> expression was significantly induced by salt stress and abscisic acid (ABA) treatment in the roots and leaves of <i>T</i>. <i>hispida</i>. ThHSFA1 is a nuclear-localized protein with transactivation activity at the <i>C</i>-terminus. Compared to nontransgenic plants, transgenic plants overexpressing <i>ThHSFA1</i> displayed enhanced salt tolerance and exhibited reduced reactive oxygen species (ROS) levels and increased antioxidant enzyme activity levels under salt stress. Therefore, we further concluded that <i>ThHSFA1</i> mediated the regulation of <i>ThWRKY4</i> in response to salt stress in <i>T</i>. <i>hispida</i>.https://www.mdpi.com/1422-0067/22/9/5048antioxidant enzymeheat shock elementheat shock transcription factorROSsalt stress<i>Tamarix</i> <i>hispida</i> |
| spellingShingle | Ting-Ting Sun Chao Wang Rui Liu Yu Zhang Yu-Cheng Wang Liu-Qiang Wang ThHSFA1 Confers Salt Stress Tolerance through Modulation of Reactive Oxygen Species Scavenging by Directly Regulating <i>ThWRKY4</i> International Journal of Molecular Sciences antioxidant enzyme heat shock element heat shock transcription factor ROS salt stress <i>Tamarix</i> <i>hispida</i> |
| title | ThHSFA1 Confers Salt Stress Tolerance through Modulation of Reactive Oxygen Species Scavenging by Directly Regulating <i>ThWRKY4</i> |
| title_full | ThHSFA1 Confers Salt Stress Tolerance through Modulation of Reactive Oxygen Species Scavenging by Directly Regulating <i>ThWRKY4</i> |
| title_fullStr | ThHSFA1 Confers Salt Stress Tolerance through Modulation of Reactive Oxygen Species Scavenging by Directly Regulating <i>ThWRKY4</i> |
| title_full_unstemmed | ThHSFA1 Confers Salt Stress Tolerance through Modulation of Reactive Oxygen Species Scavenging by Directly Regulating <i>ThWRKY4</i> |
| title_short | ThHSFA1 Confers Salt Stress Tolerance through Modulation of Reactive Oxygen Species Scavenging by Directly Regulating <i>ThWRKY4</i> |
| title_sort | thhsfa1 confers salt stress tolerance through modulation of reactive oxygen species scavenging by directly regulating i thwrky4 i |
| topic | antioxidant enzyme heat shock element heat shock transcription factor ROS salt stress <i>Tamarix</i> <i>hispida</i> |
| url | https://www.mdpi.com/1422-0067/22/9/5048 |
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