Vitamin D Ameliorates the Hepatic Oxidative Damage and Fibrotic Effect Caused by Thioacetamide in Rats
Vitamin D<sub>3</sub> (VD<sub>3</sub>) is a sunshine hormone that regulates cellular proliferation, differentiation, apoptosis, and angiogenesis related to liver parenchyma. We used a thioacetamide (TAA)-induced hepatic fibrosis rat model in our study to investigate the benef...
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MDPI AG
2023-02-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/11/2/424 |
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| author | Aya Megahed Hossam Gadalla Fatma M. Abdelhamid Samah J. Almehmadi Anmar A. Khan Talat A. Albukhari Engy F. Risha |
| author_facet | Aya Megahed Hossam Gadalla Fatma M. Abdelhamid Samah J. Almehmadi Anmar A. Khan Talat A. Albukhari Engy F. Risha |
| author_sort | Aya Megahed |
| collection | DOAJ |
| description | Vitamin D<sub>3</sub> (VD<sub>3</sub>) is a sunshine hormone that regulates cellular proliferation, differentiation, apoptosis, and angiogenesis related to liver parenchyma. We used a thioacetamide (TAA)-induced hepatic fibrosis rat model in our study to investigate the beneficial roles of VD<sub>3</sub> to overcome extensive liver fibrosis. Randomly, four equal groups (eight rats per group) underwent therapy for eight successive weeks: a control group, a group treated with TAA 100 mg/kg BW IP every other day, a group treated with VD<sub>3</sub> 1000 IU/kg BW IM every day, and a TAA+VD group treated with both therapies. Treatment with VD<sub>3</sub> after TAA-induced hepatic fibrosis was found to alleviate elevated liver function measures by decreasing ALT, AST, and ALP activity; decreasing total bilirubin, direct bilirubin, cholesterol, and triglyceride levels; and increasing glucose and 25[OH]D<sub>3</sub>. Rats treated with VD<sub>3</sub> showed marked decreases in MDA and increased SOD, CAT, and GSH levels. In addition, CD34 and FGF23 gene expressions were reduced after dual therapy. Liver sections from the TAA+VD group showed markedly decreased hepatic lesions, and Masson’s trichrome stain showed a marked decrease in dense bluish-stained fibrous tissue. The immunohistochemical expression of TGF-β and α-SMA showed markedly decreased positive brown cytoplasmic expression in a few hepatocytes, clarifying the antifibrotic effect of VD<sub>3</sub> in hepatic fibrosis. In conclusion, VD<sub>3</sub> alleviates hepatotoxicity and fibrosis caused by TAA. |
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| last_indexed | 2024-03-11T09:06:37Z |
| publishDate | 2023-02-01 |
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| series | Biomedicines |
| spelling | doaj.art-0f293583404c4742b872a790ebc077802023-11-16T19:18:01ZengMDPI AGBiomedicines2227-90592023-02-0111242410.3390/biomedicines11020424Vitamin D Ameliorates the Hepatic Oxidative Damage and Fibrotic Effect Caused by Thioacetamide in RatsAya Megahed0Hossam Gadalla1Fatma M. Abdelhamid2Samah J. Almehmadi3Anmar A. Khan4Talat A. Albukhari5Engy F. Risha6Department of Clinical Pathology, Faculty of Veterinary Medicine, Mansoura University, Mansour 35516, EgyptDepartment of Clinical Pathology, Faculty of Veterinary Medicine, Mansoura University, Mansour 35516, EgyptDepartment of Clinical Pathology, Faculty of Veterinary Medicine, Mansoura University, Mansour 35516, EgyptLaboratory Medicine Department, Faculty of Applied Medical Sciences, Umm Al-Qura University, Al Abdeyah, Makkah P.O. Box 7607, Saudi ArabiaLaboratory Medicine Department, Faculty of Applied Medical Sciences, Umm Al-Qura University, Al Abdeyah, Makkah P.O. Box 7607, Saudi ArabiaDepartment of Immunology and Hematology, Faculty of Medicine, Umm Al-Qura University, Makkah P.O. Box 7607, Saudi ArabiaDepartment of Clinical Pathology, Faculty of Veterinary Medicine, Mansoura University, Mansour 35516, EgyptVitamin D<sub>3</sub> (VD<sub>3</sub>) is a sunshine hormone that regulates cellular proliferation, differentiation, apoptosis, and angiogenesis related to liver parenchyma. We used a thioacetamide (TAA)-induced hepatic fibrosis rat model in our study to investigate the beneficial roles of VD<sub>3</sub> to overcome extensive liver fibrosis. Randomly, four equal groups (eight rats per group) underwent therapy for eight successive weeks: a control group, a group treated with TAA 100 mg/kg BW IP every other day, a group treated with VD<sub>3</sub> 1000 IU/kg BW IM every day, and a TAA+VD group treated with both therapies. Treatment with VD<sub>3</sub> after TAA-induced hepatic fibrosis was found to alleviate elevated liver function measures by decreasing ALT, AST, and ALP activity; decreasing total bilirubin, direct bilirubin, cholesterol, and triglyceride levels; and increasing glucose and 25[OH]D<sub>3</sub>. Rats treated with VD<sub>3</sub> showed marked decreases in MDA and increased SOD, CAT, and GSH levels. In addition, CD34 and FGF23 gene expressions were reduced after dual therapy. Liver sections from the TAA+VD group showed markedly decreased hepatic lesions, and Masson’s trichrome stain showed a marked decrease in dense bluish-stained fibrous tissue. The immunohistochemical expression of TGF-β and α-SMA showed markedly decreased positive brown cytoplasmic expression in a few hepatocytes, clarifying the antifibrotic effect of VD<sub>3</sub> in hepatic fibrosis. In conclusion, VD<sub>3</sub> alleviates hepatotoxicity and fibrosis caused by TAA.https://www.mdpi.com/2227-9059/11/2/424vitamin D<sub>3</sub>thioacetamidehepatic oxidative stressfibrosis scoringfibrotic markers |
| spellingShingle | Aya Megahed Hossam Gadalla Fatma M. Abdelhamid Samah J. Almehmadi Anmar A. Khan Talat A. Albukhari Engy F. Risha Vitamin D Ameliorates the Hepatic Oxidative Damage and Fibrotic Effect Caused by Thioacetamide in Rats Biomedicines vitamin D<sub>3</sub> thioacetamide hepatic oxidative stress fibrosis scoring fibrotic markers |
| title | Vitamin D Ameliorates the Hepatic Oxidative Damage and Fibrotic Effect Caused by Thioacetamide in Rats |
| title_full | Vitamin D Ameliorates the Hepatic Oxidative Damage and Fibrotic Effect Caused by Thioacetamide in Rats |
| title_fullStr | Vitamin D Ameliorates the Hepatic Oxidative Damage and Fibrotic Effect Caused by Thioacetamide in Rats |
| title_full_unstemmed | Vitamin D Ameliorates the Hepatic Oxidative Damage and Fibrotic Effect Caused by Thioacetamide in Rats |
| title_short | Vitamin D Ameliorates the Hepatic Oxidative Damage and Fibrotic Effect Caused by Thioacetamide in Rats |
| title_sort | vitamin d ameliorates the hepatic oxidative damage and fibrotic effect caused by thioacetamide in rats |
| topic | vitamin D<sub>3</sub> thioacetamide hepatic oxidative stress fibrosis scoring fibrotic markers |
| url | https://www.mdpi.com/2227-9059/11/2/424 |
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