Genetic and Biochemical Characterization of an Exopolysaccharide With in vitro Antitumoral Activity Produced by Lactobacillus fermentum YL-11
In the present study, the whole genome sequence of Lactobacillus fermentum YL-11, a novel exopolysaccharide (EPS)-producing lactic acid bacteria (LAB) strain isolated from fermented milk, was determined. Genetic information and the synthetic mechanism of the EPS in L. fermentum YL-11 were identified...
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| Format: | Article |
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Frontiers Media S.A.
2019-12-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.02898/full |
| _version_ | 1818510291059605504 |
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| author | Yunlu Wei Yunlu Wei Fei Li Fei Li Le Li Linlin Huang Linlin Huang Quanhong Li Quanhong Li |
| author_facet | Yunlu Wei Yunlu Wei Fei Li Fei Li Le Li Linlin Huang Linlin Huang Quanhong Li Quanhong Li |
| author_sort | Yunlu Wei |
| collection | DOAJ |
| description | In the present study, the whole genome sequence of Lactobacillus fermentum YL-11, a novel exopolysaccharide (EPS)-producing lactic acid bacteria (LAB) strain isolated from fermented milk, was determined. Genetic information and the synthetic mechanism of the EPS in L. fermentum YL-11 were identified based on bioinformatic analysis of the complete genome. The purified EPS of YL-11 mainly comprised galactose (48.0%), glucose (30.3%), mannose (11.8%), and arabinose (6.0%). In vitro, the EPS from YL-11 exhibited inhibition activity against HT-29 and Caco-2 colon cancer cells, suggesting that EPS from strain YL-11 might be used as an antitumoral agent. EPS at 600 and 800 μg/mL achieved inhibition rates of 46.5 ± 3.5% and 45.6 ± 6.1% to HT-29 cells, respectively. The genomic information about L. fermentum YL-11 and the antitumoral activity of YL-11 EPS provide a theoretical foundation for the future application of EPS in the food and pharmaceutical industries. |
| first_indexed | 2024-12-10T22:57:13Z |
| format | Article |
| id | doaj.art-0f2ade6fe55243c7b51a5426938f4665 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-10T22:57:13Z |
| publishDate | 2019-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-0f2ade6fe55243c7b51a5426938f46652022-12-22T01:30:15ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-12-011010.3389/fmicb.2019.02898445549Genetic and Biochemical Characterization of an Exopolysaccharide With in vitro Antitumoral Activity Produced by Lactobacillus fermentum YL-11Yunlu Wei0Yunlu Wei1Fei Li2Fei Li3Le Li4Linlin Huang5Linlin Huang6Quanhong Li7Quanhong Li8College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, ChinaNational Engineering Research Center for Fruit and Vegetable Processing, Beijing, ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing, ChinaNational Engineering Research Center for Fruit and Vegetable Processing, Beijing, ChinaDepartment of Environmental and Quality Inspection, Chongqing Chemical Industry Vocational College, Chongqing, ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing, ChinaNational Engineering Research Center for Fruit and Vegetable Processing, Beijing, ChinaCollege of Food Science and Nutritional Engineering, China Agricultural University, Beijing, ChinaNational Engineering Research Center for Fruit and Vegetable Processing, Beijing, ChinaIn the present study, the whole genome sequence of Lactobacillus fermentum YL-11, a novel exopolysaccharide (EPS)-producing lactic acid bacteria (LAB) strain isolated from fermented milk, was determined. Genetic information and the synthetic mechanism of the EPS in L. fermentum YL-11 were identified based on bioinformatic analysis of the complete genome. The purified EPS of YL-11 mainly comprised galactose (48.0%), glucose (30.3%), mannose (11.8%), and arabinose (6.0%). In vitro, the EPS from YL-11 exhibited inhibition activity against HT-29 and Caco-2 colon cancer cells, suggesting that EPS from strain YL-11 might be used as an antitumoral agent. EPS at 600 and 800 μg/mL achieved inhibition rates of 46.5 ± 3.5% and 45.6 ± 6.1% to HT-29 cells, respectively. The genomic information about L. fermentum YL-11 and the antitumoral activity of YL-11 EPS provide a theoretical foundation for the future application of EPS in the food and pharmaceutical industries.https://www.frontiersin.org/article/10.3389/fmicb.2019.02898/fullLactobacillus fermentumexopolysaccharidewhole genome sequencebiosynthetic mechanismantitumoral activity |
| spellingShingle | Yunlu Wei Yunlu Wei Fei Li Fei Li Le Li Linlin Huang Linlin Huang Quanhong Li Quanhong Li Genetic and Biochemical Characterization of an Exopolysaccharide With in vitro Antitumoral Activity Produced by Lactobacillus fermentum YL-11 Frontiers in Microbiology Lactobacillus fermentum exopolysaccharide whole genome sequence biosynthetic mechanism antitumoral activity |
| title | Genetic and Biochemical Characterization of an Exopolysaccharide With in vitro Antitumoral Activity Produced by Lactobacillus fermentum YL-11 |
| title_full | Genetic and Biochemical Characterization of an Exopolysaccharide With in vitro Antitumoral Activity Produced by Lactobacillus fermentum YL-11 |
| title_fullStr | Genetic and Biochemical Characterization of an Exopolysaccharide With in vitro Antitumoral Activity Produced by Lactobacillus fermentum YL-11 |
| title_full_unstemmed | Genetic and Biochemical Characterization of an Exopolysaccharide With in vitro Antitumoral Activity Produced by Lactobacillus fermentum YL-11 |
| title_short | Genetic and Biochemical Characterization of an Exopolysaccharide With in vitro Antitumoral Activity Produced by Lactobacillus fermentum YL-11 |
| title_sort | genetic and biochemical characterization of an exopolysaccharide with in vitro antitumoral activity produced by lactobacillus fermentum yl 11 |
| topic | Lactobacillus fermentum exopolysaccharide whole genome sequence biosynthetic mechanism antitumoral activity |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.02898/full |
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