Transcriptome and metabolomics analysis revealed that CmWRKY49 regulating CmPSY1 promotes β-carotene accumulation in orange fleshed oriental melon
The flesh color of oriental melons is an important commercial trait that affects consumer preferences. To explore the mechanisms underlying the flesh color formation and regulation during fruit ripening, carotenoid-targeted metabolomic and RNA-seq analysis were conducted between white-fleshed (WF) a...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2022-09-01
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| Series: | Horticultural Plant Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468014122000802 |
| _version_ | 1797203822356987904 |
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| author | Xiaoyu Duan Cai Jiang Yaping Zhao Ge Gao Meng Li Hongyan Qi |
| author_facet | Xiaoyu Duan Cai Jiang Yaping Zhao Ge Gao Meng Li Hongyan Qi |
| author_sort | Xiaoyu Duan |
| collection | DOAJ |
| description | The flesh color of oriental melons is an important commercial trait that affects consumer preferences. To explore the mechanisms underlying the flesh color formation and regulation during fruit ripening, carotenoid-targeted metabolomic and RNA-seq analysis were conducted between white-fleshed (WF) and orange-fleshed (OF) oriental melon cultivars at different stages. The carotenoid-targeted metabolomic analysis indicated that β-carotene was the major metabolite that caused differences in flesh color between the two cultivars. Additionally, through KEGG pathway enrichment and weighted gene co-expression network (WGCNA) analysis, metabolic pathways and related transcription factors that are associated with carotenoid metabolism were selected and transcriptome data was verified using RT-qPCR. Finally, the yeast one hybrid and luciferase activity showed that the transcription factor CmWRKY49 could directly bind to the CmPSY1 promoter to activate its expression in the ‘OF’ cultivar. Transient overexpression of CmWRKY49 in ‘OF’ cultivar increased the β-carotene content, while the β-carotene content decreased when it was silenced in the same cultivar. This study provides insights into the underlying regulatory network of carotenoid metabolism in oriental melon fruit. |
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| id | doaj.art-0f2c2da5664c48fd930a25f775eb6262 |
| institution | Directory Open Access Journal |
| issn | 2468-0141 |
| language | English |
| last_indexed | 2024-04-24T08:25:26Z |
| publishDate | 2022-09-01 |
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| series | Horticultural Plant Journal |
| spelling | doaj.art-0f2c2da5664c48fd930a25f775eb62622024-04-16T22:22:14ZengKeAi Communications Co., Ltd.Horticultural Plant Journal2468-01412022-09-0185650666Transcriptome and metabolomics analysis revealed that CmWRKY49 regulating CmPSY1 promotes β-carotene accumulation in orange fleshed oriental melonXiaoyu Duan0Cai Jiang1Yaping Zhao2Ge Gao3Meng Li4Hongyan Qi5Key Laboratory of Protected Horticulture of Education Ministry and Liaoning Province, College of Horticulture, Shenyang Agricultural University, National and Local Joint Engineering Research Centre of Northern Horticultural, Facilities Design and Application Technology (Liaoning), Shenyang 110866, ChinaNanodigmbio Biotechnology Co., Ltd. Nanjing 210038, ChinaKey Laboratory of Protected Horticulture of Education Ministry and Liaoning Province, College of Horticulture, Shenyang Agricultural University, National and Local Joint Engineering Research Centre of Northern Horticultural, Facilities Design and Application Technology (Liaoning), Shenyang 110866, ChinaKey Laboratory of Protected Horticulture of Education Ministry and Liaoning Province, College of Horticulture, Shenyang Agricultural University, National and Local Joint Engineering Research Centre of Northern Horticultural, Facilities Design and Application Technology (Liaoning), Shenyang 110866, ChinaKey Laboratory of Protected Horticulture of Education Ministry and Liaoning Province, College of Horticulture, Shenyang Agricultural University, National and Local Joint Engineering Research Centre of Northern Horticultural, Facilities Design and Application Technology (Liaoning), Shenyang 110866, ChinaKey Laboratory of Protected Horticulture of Education Ministry and Liaoning Province, College of Horticulture, Shenyang Agricultural University, National and Local Joint Engineering Research Centre of Northern Horticultural, Facilities Design and Application Technology (Liaoning), Shenyang 110866, China; Corresponding author.The flesh color of oriental melons is an important commercial trait that affects consumer preferences. To explore the mechanisms underlying the flesh color formation and regulation during fruit ripening, carotenoid-targeted metabolomic and RNA-seq analysis were conducted between white-fleshed (WF) and orange-fleshed (OF) oriental melon cultivars at different stages. The carotenoid-targeted metabolomic analysis indicated that β-carotene was the major metabolite that caused differences in flesh color between the two cultivars. Additionally, through KEGG pathway enrichment and weighted gene co-expression network (WGCNA) analysis, metabolic pathways and related transcription factors that are associated with carotenoid metabolism were selected and transcriptome data was verified using RT-qPCR. Finally, the yeast one hybrid and luciferase activity showed that the transcription factor CmWRKY49 could directly bind to the CmPSY1 promoter to activate its expression in the ‘OF’ cultivar. Transient overexpression of CmWRKY49 in ‘OF’ cultivar increased the β-carotene content, while the β-carotene content decreased when it was silenced in the same cultivar. This study provides insights into the underlying regulatory network of carotenoid metabolism in oriental melon fruit.http://www.sciencedirect.com/science/article/pii/S2468014122000802Oriental melonCarotenoidTranscriptome analysisWRKYCmPSY1 |
| spellingShingle | Xiaoyu Duan Cai Jiang Yaping Zhao Ge Gao Meng Li Hongyan Qi Transcriptome and metabolomics analysis revealed that CmWRKY49 regulating CmPSY1 promotes β-carotene accumulation in orange fleshed oriental melon Horticultural Plant Journal Oriental melon Carotenoid Transcriptome analysis WRKY CmPSY1 |
| title | Transcriptome and metabolomics analysis revealed that CmWRKY49 regulating CmPSY1 promotes β-carotene accumulation in orange fleshed oriental melon |
| title_full | Transcriptome and metabolomics analysis revealed that CmWRKY49 regulating CmPSY1 promotes β-carotene accumulation in orange fleshed oriental melon |
| title_fullStr | Transcriptome and metabolomics analysis revealed that CmWRKY49 regulating CmPSY1 promotes β-carotene accumulation in orange fleshed oriental melon |
| title_full_unstemmed | Transcriptome and metabolomics analysis revealed that CmWRKY49 regulating CmPSY1 promotes β-carotene accumulation in orange fleshed oriental melon |
| title_short | Transcriptome and metabolomics analysis revealed that CmWRKY49 regulating CmPSY1 promotes β-carotene accumulation in orange fleshed oriental melon |
| title_sort | transcriptome and metabolomics analysis revealed that cmwrky49 regulating cmpsy1 promotes β carotene accumulation in orange fleshed oriental melon |
| topic | Oriental melon Carotenoid Transcriptome analysis WRKY CmPSY1 |
| url | http://www.sciencedirect.com/science/article/pii/S2468014122000802 |
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