OPTIMIZATION OF A CULTURE MEDIUM FOR BIOMASS AND δ-ENDOTOXIN PRODUCTION BY A RECOMBINANT ESCHERICHIA COLI STRAIN
A recombinant strain of Escherichia coli harboring a plasmid containing the Bacillus thuringiensis δ-endotoxin synthesis gene, was tested for its efficacy to synthesize δ-endotoxin, in a complex medium containing sucrose and yeast extract. Also, the recombinant E. coli strain was tested for its effi...
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| Format: | Article |
| Language: | Arabic |
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The Union of Arab Universities
2008-03-01
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| Series: | Arab Universities Journal of Agricultural Sciences |
| Subjects: | |
| Online Access: | https://ajs.journals.ekb.eg/article_14535_c511d0a00466b9eb1484083007eb148c.pdf |
| _version_ | 1797269643198464000 |
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| author | Rawia F. Gamal Al-Fateh R. Abeer Mohammed M. El-Sawy |
| author_facet | Rawia F. Gamal Al-Fateh R. Abeer Mohammed M. El-Sawy |
| author_sort | Rawia F. Gamal |
| collection | DOAJ |
| description | A recombinant strain of Escherichia coli harboring a plasmid containing the Bacillus thuringiensis δ-endotoxin synthesis gene, was tested for its efficacy to synthesize δ-endotoxin, in a complex medium containing sucrose and yeast extract. Also, the recombinant E. coli strain was tested for its efficacy against the 2nd instars of Spodoptera littoralis. The recombinant strain of E. coli showed a good activity against the 2nd instars of S. littoralis, the mortality was 70 % after 7 days at room temperature. A high cell biomass (8.8gL-1) and δ-endotoxin concentration (6.8 mg L-1), were obtained by the shake flask culture (100 ml medium/250 ml flask, at 200 rpm), of the recombinant E. coli in modified MR medium containing sucrose (20g/L), as carbon source and yeast extract as nitrogen source, in the presence of CaCO3, K2HPO4, MgSO4, FeSO4 and ZnSO4 as mineral salts. The best pH values for cell biomass production and endotoxin production were 7.0 and 7.5, respectively. The corresponding figures for the best temperature were 37oC and 30oC, respectively. The use of some byproducts such as black-strap molasses, corn-steep liquor and cheese whey, as an alternative for carbon and nitrogen sources of medium, were found to enhance the cell growth but showed no effect on endotoxin production. |
| first_indexed | 2024-04-25T01:51:38Z |
| format | Article |
| id | doaj.art-0f3373846fc14d2897c539d48c263063 |
| institution | Directory Open Access Journal |
| issn | 1110-2675 2636-3585 |
| language | Arabic |
| last_indexed | 2024-04-25T01:51:38Z |
| publishDate | 2008-03-01 |
| publisher | The Union of Arab Universities |
| record_format | Article |
| series | Arab Universities Journal of Agricultural Sciences |
| spelling | doaj.art-0f3373846fc14d2897c539d48c2630632024-03-07T17:45:59ZaraThe Union of Arab UniversitiesArab Universities Journal of Agricultural Sciences1110-26752636-35852008-03-01161415510.21608/ajs.2008.1453514535OPTIMIZATION OF A CULTURE MEDIUM FOR BIOMASS AND δ-ENDOTOXIN PRODUCTION BY A RECOMBINANT ESCHERICHIA COLI STRAINRawia F. Gamal0Al-Fateh R.1Abeer Mohammed2M. El-Sawy3Dept. Microbiol., Fac. Agric., Ain Shams University, Shobra El-Khema, Cairo, EgyptPlant Protection Research Institute, Agric. Research Center, Dokki, Giza, EgyptPlant Protection Research Institute, Agric. Research Center, Dokki, Giza, EgyptDept. Microbiol., Fac. Agric., Ain Shams University, Shobra El-Khema, Cairo, EgyptA recombinant strain of Escherichia coli harboring a plasmid containing the Bacillus thuringiensis δ-endotoxin synthesis gene, was tested for its efficacy to synthesize δ-endotoxin, in a complex medium containing sucrose and yeast extract. Also, the recombinant E. coli strain was tested for its efficacy against the 2nd instars of Spodoptera littoralis. The recombinant strain of E. coli showed a good activity against the 2nd instars of S. littoralis, the mortality was 70 % after 7 days at room temperature. A high cell biomass (8.8gL-1) and δ-endotoxin concentration (6.8 mg L-1), were obtained by the shake flask culture (100 ml medium/250 ml flask, at 200 rpm), of the recombinant E. coli in modified MR medium containing sucrose (20g/L), as carbon source and yeast extract as nitrogen source, in the presence of CaCO3, K2HPO4, MgSO4, FeSO4 and ZnSO4 as mineral salts. The best pH values for cell biomass production and endotoxin production were 7.0 and 7.5, respectively. The corresponding figures for the best temperature were 37oC and 30oC, respectively. The use of some byproducts such as black-strap molasses, corn-steep liquor and cheese whey, as an alternative for carbon and nitrogen sources of medium, were found to enhance the cell growth but showed no effect on endotoxin production.https://ajs.journals.ekb.eg/article_14535_c511d0a00466b9eb1484083007eb148c.pdfrecombinant escherichia colibiomassδ-endotoxin productionmedium optimization |
| spellingShingle | Rawia F. Gamal Al-Fateh R. Abeer Mohammed M. El-Sawy OPTIMIZATION OF A CULTURE MEDIUM FOR BIOMASS AND δ-ENDOTOXIN PRODUCTION BY A RECOMBINANT ESCHERICHIA COLI STRAIN Arab Universities Journal of Agricultural Sciences recombinant escherichia coli biomass δ-endotoxin production medium optimization |
| title | OPTIMIZATION OF A CULTURE MEDIUM FOR BIOMASS AND δ-ENDOTOXIN PRODUCTION BY A RECOMBINANT ESCHERICHIA COLI STRAIN |
| title_full | OPTIMIZATION OF A CULTURE MEDIUM FOR BIOMASS AND δ-ENDOTOXIN PRODUCTION BY A RECOMBINANT ESCHERICHIA COLI STRAIN |
| title_fullStr | OPTIMIZATION OF A CULTURE MEDIUM FOR BIOMASS AND δ-ENDOTOXIN PRODUCTION BY A RECOMBINANT ESCHERICHIA COLI STRAIN |
| title_full_unstemmed | OPTIMIZATION OF A CULTURE MEDIUM FOR BIOMASS AND δ-ENDOTOXIN PRODUCTION BY A RECOMBINANT ESCHERICHIA COLI STRAIN |
| title_short | OPTIMIZATION OF A CULTURE MEDIUM FOR BIOMASS AND δ-ENDOTOXIN PRODUCTION BY A RECOMBINANT ESCHERICHIA COLI STRAIN |
| title_sort | optimization of a culture medium for biomass and δ endotoxin production by a recombinant escherichia coli strain |
| topic | recombinant escherichia coli biomass δ-endotoxin production medium optimization |
| url | https://ajs.journals.ekb.eg/article_14535_c511d0a00466b9eb1484083007eb148c.pdf |
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