3D Filaments Based on Polyhydroxy Butyrate—Micronized Bacterial Cellulose for Tissue Engineering Applications
In this work, scaffolds based on poly(hydroxybutyrate) (PHB) and micronized bacterial cellulose (BC) were produced through 3D printing. Filaments for the printing were obtained by varying the percentage of micronized BC (0.25, 0.50, 1.00, and 2.00%) inserted in relation to the PHB matrix. Despite th...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | https://www.mdpi.com/2079-4983/14/9/464 |
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| author | Matheus F. Celestino Lais R. Lima Marina Fontes Igor T. S. Batista Daniella R. Mulinari Alessandra Dametto Raphael A. Rattes André C. Amaral Rosana M. N. Assunção Clovis A. Ribeiro Guillermo R. Castro Hernane S. Barud |
| author_facet | Matheus F. Celestino Lais R. Lima Marina Fontes Igor T. S. Batista Daniella R. Mulinari Alessandra Dametto Raphael A. Rattes André C. Amaral Rosana M. N. Assunção Clovis A. Ribeiro Guillermo R. Castro Hernane S. Barud |
| author_sort | Matheus F. Celestino |
| collection | DOAJ |
| description | In this work, scaffolds based on poly(hydroxybutyrate) (PHB) and micronized bacterial cellulose (BC) were produced through 3D printing. Filaments for the printing were obtained by varying the percentage of micronized BC (0.25, 0.50, 1.00, and 2.00%) inserted in relation to the PHB matrix. Despite the varying concentrations of BC, the biocomposite filaments predominantly contained PHB functional groups, as Fourier transform infrared spectroscopy (FTIR) demonstrated. Thermogravimetric analyses (i.e., TG and DTG) of the filaments showed that the peak temperature (T<sub>peak</sub>) of PHB degradation decreased as the concentration of BC increased, with the lowest being 248 °C, referring to the biocomposite filament PHB/2.0% BC, which has the highest concentration of BC. Although there was a variation in the thermal behavior of the filaments, it was not significant enough to make printing impossible, considering that the PHB melting temperature was 170 °C. Biological assays indicated the non-cytotoxicity of scaffolds and the provision of cell anchorage sites. The results obtained in this research open up new paths for the application of this innovation in tissue engineering. |
| first_indexed | 2024-03-10T22:35:47Z |
| format | Article |
| id | doaj.art-0f339caec55847f79902c1be69bb3b71 |
| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-03-10T22:35:47Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-0f339caec55847f79902c1be69bb3b712023-11-19T11:23:28ZengMDPI AGJournal of Functional Biomaterials2079-49832023-09-0114946410.3390/jfb140904643D Filaments Based on Polyhydroxy Butyrate—Micronized Bacterial Cellulose for Tissue Engineering ApplicationsMatheus F. Celestino0Lais R. Lima1Marina Fontes2Igor T. S. Batista3Daniella R. Mulinari4Alessandra Dametto5Raphael A. Rattes6André C. Amaral7Rosana M. N. Assunção8Clovis A. Ribeiro9Guillermo R. Castro10Hernane S. Barud11Biopolymers and Biomaterials Group, Postgraduate Program in Biotechnology, University of Araraquara (UNIARA), Araraquara 14801-320, SP, BrazilInstitute of Chemistry, University of São Paulo (USP), São Carlos 13566-590, SP, BrazilBiopolymers and Biomaterials Group, Postgraduate Program in Biotechnology, University of Araraquara (UNIARA), Araraquara 14801-320, SP, BrazilBiopolymers and Biomaterials Group, Postgraduate Program in Biotechnology, University of Araraquara (UNIARA), Araraquara 14801-320, SP, BrazilDepartment of Mechanics and Energy, State University of Rio de Janeiro (UEJR), Rio de Janeiro 20550-900, RJ, BrazilBiosmart Nanotechnology LTDA, Araraquara 14808-162, SP, BrazilBiopolymers and Biomaterials Group, Postgraduate Program in Biotechnology, University of Araraquara (UNIARA), Araraquara 14801-320, SP, BrazilBiopolymers and Biomaterials Group, Postgraduate Program in Biotechnology, University of Araraquara (UNIARA), Araraquara 14801-320, SP, BrazilFaculty of Integrated Sciences of Pontal (FACIP), Federal University of Uberlandia (UFU), Pontal Campus, Ituiutaba 38304-402, MG, BrazilInstitute of Chemistry, São Paulo State University (UNESP), Araraquara 14800-900, SP, BrazilNanomedicine Research Unit (Nanomed), Center for Natural and Human Sciences, Federal University of ABC (UFABC), Santo André 09210-580, SP, BrazilBiopolymers and Biomaterials Group, Postgraduate Program in Biotechnology, University of Araraquara (UNIARA), Araraquara 14801-320, SP, BrazilIn this work, scaffolds based on poly(hydroxybutyrate) (PHB) and micronized bacterial cellulose (BC) were produced through 3D printing. Filaments for the printing were obtained by varying the percentage of micronized BC (0.25, 0.50, 1.00, and 2.00%) inserted in relation to the PHB matrix. Despite the varying concentrations of BC, the biocomposite filaments predominantly contained PHB functional groups, as Fourier transform infrared spectroscopy (FTIR) demonstrated. Thermogravimetric analyses (i.e., TG and DTG) of the filaments showed that the peak temperature (T<sub>peak</sub>) of PHB degradation decreased as the concentration of BC increased, with the lowest being 248 °C, referring to the biocomposite filament PHB/2.0% BC, which has the highest concentration of BC. Although there was a variation in the thermal behavior of the filaments, it was not significant enough to make printing impossible, considering that the PHB melting temperature was 170 °C. Biological assays indicated the non-cytotoxicity of scaffolds and the provision of cell anchorage sites. The results obtained in this research open up new paths for the application of this innovation in tissue engineering.https://www.mdpi.com/2079-4983/14/9/4643D printingmicronized bacterial cellulosepoly(hydroxybutyrate)tissue engineeringscaffolds |
| spellingShingle | Matheus F. Celestino Lais R. Lima Marina Fontes Igor T. S. Batista Daniella R. Mulinari Alessandra Dametto Raphael A. Rattes André C. Amaral Rosana M. N. Assunção Clovis A. Ribeiro Guillermo R. Castro Hernane S. Barud 3D Filaments Based on Polyhydroxy Butyrate—Micronized Bacterial Cellulose for Tissue Engineering Applications Journal of Functional Biomaterials 3D printing micronized bacterial cellulose poly(hydroxybutyrate) tissue engineering scaffolds |
| title | 3D Filaments Based on Polyhydroxy Butyrate—Micronized Bacterial Cellulose for Tissue Engineering Applications |
| title_full | 3D Filaments Based on Polyhydroxy Butyrate—Micronized Bacterial Cellulose for Tissue Engineering Applications |
| title_fullStr | 3D Filaments Based on Polyhydroxy Butyrate—Micronized Bacterial Cellulose for Tissue Engineering Applications |
| title_full_unstemmed | 3D Filaments Based on Polyhydroxy Butyrate—Micronized Bacterial Cellulose for Tissue Engineering Applications |
| title_short | 3D Filaments Based on Polyhydroxy Butyrate—Micronized Bacterial Cellulose for Tissue Engineering Applications |
| title_sort | 3d filaments based on polyhydroxy butyrate micronized bacterial cellulose for tissue engineering applications |
| topic | 3D printing micronized bacterial cellulose poly(hydroxybutyrate) tissue engineering scaffolds |
| url | https://www.mdpi.com/2079-4983/14/9/464 |
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