3,5-Diiodo-L-Thyronine Modifies the Lipid Droplet Composition in a Model of Hepatosteatosis
Background/Aims: Fatty acids are the main energy stores and the major membrane components of the cells. In the hepatocyte, fatty acids are esterified to triacylglycerols (TAGs) and stored in lipid droplets (LDs). The lipid lowering action of 3,5-diiodo-L-thyronine (T2) on an in vitro model of hepato...
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| Format: | Article |
| Language: | English |
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Cell Physiol Biochem Press GmbH & Co KG
2014-02-01
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| Series: | Cellular Physiology and Biochemistry |
| Subjects: | |
| Online Access: | http://www.karger.com/Article/FullText/356674 |
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| author | Elena Grasselli Adriana Voci Laura Canesi Annalisa Salis Gianluca Damonte Andrea D. Compalati Fernando Goglia Gabriella Gallo Laura Vergani |
| author_facet | Elena Grasselli Adriana Voci Laura Canesi Annalisa Salis Gianluca Damonte Andrea D. Compalati Fernando Goglia Gabriella Gallo Laura Vergani |
| author_sort | Elena Grasselli |
| collection | DOAJ |
| description | Background/Aims: Fatty acids are the main energy stores and the major membrane components of the cells. In the hepatocyte, fatty acids are esterified to triacylglycerols (TAGs) and stored in lipid droplets (LDs). The lipid lowering action of 3,5-diiodo-L-thyronine (T2) on an in vitro model of hepatosteatosis was investigated in terms of fatty acid and protein content of LDs, lipid oxidation and secretion. Methods: FaO cells were exposed to oleate/palmitate, then treated with T2. Results: T2 reduced number and size of LDs, and modified their acyl composition by decreasing the content of saturated (SFA) vs monounsaturated (MUFA) fatty acids thus reversing the SFA/MUFA ratio. The expression of the LD-associated proteins adipose differentiation-related protein (ADRP), oxidative tissue-enriched PAT protein (OXPAT), and adipose triglyceride lipase (ATGL) was increased in ‘steatotic' cells and further up-regulated by T2. Moreover, T2 stimulated the mitochondrial oxidation by up-regulating carnitine-palmitoyl-transferase (CPT1), uncoupling protein 2 (UCP2) and very long-chain acyl-coenzyme A dehydrogenase (VLCAD). Conclusions: T2 leads to mobilization of TAGs from LDs and stimulates mitochondrial oxidative metabolism of fatty acids, in particular of SFAs, and thus enriches of MUFAs the LDs. This action may protect the hepatocyte from excess of SFAs that are more toxic than MUFAs. |
| first_indexed | 2024-04-12T01:11:45Z |
| format | Article |
| id | doaj.art-0f34c970657f47d29cf6bc22c9d31e63 |
| institution | Directory Open Access Journal |
| issn | 1015-8987 1421-9778 |
| language | English |
| last_indexed | 2024-04-12T01:11:45Z |
| publishDate | 2014-02-01 |
| publisher | Cell Physiol Biochem Press GmbH & Co KG |
| record_format | Article |
| series | Cellular Physiology and Biochemistry |
| spelling | doaj.art-0f34c970657f47d29cf6bc22c9d31e632022-12-22T03:54:04ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782014-02-0133234435610.1159/0003566743566743,5-Diiodo-L-Thyronine Modifies the Lipid Droplet Composition in a Model of HepatosteatosisElena GrasselliAdriana VociLaura CanesiAnnalisa SalisGianluca DamonteAndrea D. CompalatiFernando GogliaGabriella GalloLaura VerganiBackground/Aims: Fatty acids are the main energy stores and the major membrane components of the cells. In the hepatocyte, fatty acids are esterified to triacylglycerols (TAGs) and stored in lipid droplets (LDs). The lipid lowering action of 3,5-diiodo-L-thyronine (T2) on an in vitro model of hepatosteatosis was investigated in terms of fatty acid and protein content of LDs, lipid oxidation and secretion. Methods: FaO cells were exposed to oleate/palmitate, then treated with T2. Results: T2 reduced number and size of LDs, and modified their acyl composition by decreasing the content of saturated (SFA) vs monounsaturated (MUFA) fatty acids thus reversing the SFA/MUFA ratio. The expression of the LD-associated proteins adipose differentiation-related protein (ADRP), oxidative tissue-enriched PAT protein (OXPAT), and adipose triglyceride lipase (ATGL) was increased in ‘steatotic' cells and further up-regulated by T2. Moreover, T2 stimulated the mitochondrial oxidation by up-regulating carnitine-palmitoyl-transferase (CPT1), uncoupling protein 2 (UCP2) and very long-chain acyl-coenzyme A dehydrogenase (VLCAD). Conclusions: T2 leads to mobilization of TAGs from LDs and stimulates mitochondrial oxidative metabolism of fatty acids, in particular of SFAs, and thus enriches of MUFAs the LDs. This action may protect the hepatocyte from excess of SFAs that are more toxic than MUFAs.http://www.karger.com/Article/FullText/3566743,5-Diiodo-L-thyronine (T2)Steatotic hepatocytesLipid dropletsSaturated/unsaturated fatty acidsPAT proteins |
| spellingShingle | Elena Grasselli Adriana Voci Laura Canesi Annalisa Salis Gianluca Damonte Andrea D. Compalati Fernando Goglia Gabriella Gallo Laura Vergani 3,5-Diiodo-L-Thyronine Modifies the Lipid Droplet Composition in a Model of Hepatosteatosis Cellular Physiology and Biochemistry 3,5-Diiodo-L-thyronine (T2) Steatotic hepatocytes Lipid droplets Saturated/unsaturated fatty acids PAT proteins |
| title | 3,5-Diiodo-L-Thyronine Modifies the Lipid Droplet Composition in a Model of Hepatosteatosis |
| title_full | 3,5-Diiodo-L-Thyronine Modifies the Lipid Droplet Composition in a Model of Hepatosteatosis |
| title_fullStr | 3,5-Diiodo-L-Thyronine Modifies the Lipid Droplet Composition in a Model of Hepatosteatosis |
| title_full_unstemmed | 3,5-Diiodo-L-Thyronine Modifies the Lipid Droplet Composition in a Model of Hepatosteatosis |
| title_short | 3,5-Diiodo-L-Thyronine Modifies the Lipid Droplet Composition in a Model of Hepatosteatosis |
| title_sort | 3 5 diiodo l thyronine modifies the lipid droplet composition in a model of hepatosteatosis |
| topic | 3,5-Diiodo-L-thyronine (T2) Steatotic hepatocytes Lipid droplets Saturated/unsaturated fatty acids PAT proteins |
| url | http://www.karger.com/Article/FullText/356674 |
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