Modular (de)construction of complex bacterial phenotypes by CRISPR/nCas9-assisted, multiplex cytidine base-editing
Rapid engineering of bacterial genomes is a requisite for both fundamental and applied studies. Here the authors develop an enhanced, broad-host-range cytidine base editor that enables multiplexed and efficient genome editing of Gram-negative bacteria.
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-05-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-022-30780-z |
| _version_ | 1818235022563344384 |
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| author | Daniel C. Volke Román A. Martino Ekaterina Kozaeva Andrea M. Smania Pablo I. Nikel |
| author_facet | Daniel C. Volke Román A. Martino Ekaterina Kozaeva Andrea M. Smania Pablo I. Nikel |
| author_sort | Daniel C. Volke |
| collection | DOAJ |
| description | Rapid engineering of bacterial genomes is a requisite for both fundamental and applied studies. Here the authors develop an enhanced, broad-host-range cytidine base editor that enables multiplexed and efficient genome editing of Gram-negative bacteria. |
| first_indexed | 2024-12-12T11:47:22Z |
| format | Article |
| id | doaj.art-0f3667bd732641d3a86763dfe000c762 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-12T11:47:22Z |
| publishDate | 2022-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-0f3667bd732641d3a86763dfe000c7622022-12-22T00:25:25ZengNature PortfolioNature Communications2041-17232022-05-0113111410.1038/s41467-022-30780-zModular (de)construction of complex bacterial phenotypes by CRISPR/nCas9-assisted, multiplex cytidine base-editingDaniel C. Volke0Román A. Martino1Ekaterina Kozaeva2Andrea M. Smania3Pablo I. Nikel4The Novo Nordisk Foundation Center for Biosustainability, Technical University of DenmarkDepartamento de Química Biológica Ranwel Caputto, Facultad de Ciencias Químicas, Universidad Nacional de CórdobaThe Novo Nordisk Foundation Center for Biosustainability, Technical University of DenmarkDepartamento de Química Biológica Ranwel Caputto, Facultad de Ciencias Químicas, Universidad Nacional de CórdobaThe Novo Nordisk Foundation Center for Biosustainability, Technical University of DenmarkRapid engineering of bacterial genomes is a requisite for both fundamental and applied studies. Here the authors develop an enhanced, broad-host-range cytidine base editor that enables multiplexed and efficient genome editing of Gram-negative bacteria.https://doi.org/10.1038/s41467-022-30780-z |
| spellingShingle | Daniel C. Volke Román A. Martino Ekaterina Kozaeva Andrea M. Smania Pablo I. Nikel Modular (de)construction of complex bacterial phenotypes by CRISPR/nCas9-assisted, multiplex cytidine base-editing Nature Communications |
| title | Modular (de)construction of complex bacterial phenotypes by CRISPR/nCas9-assisted, multiplex cytidine base-editing |
| title_full | Modular (de)construction of complex bacterial phenotypes by CRISPR/nCas9-assisted, multiplex cytidine base-editing |
| title_fullStr | Modular (de)construction of complex bacterial phenotypes by CRISPR/nCas9-assisted, multiplex cytidine base-editing |
| title_full_unstemmed | Modular (de)construction of complex bacterial phenotypes by CRISPR/nCas9-assisted, multiplex cytidine base-editing |
| title_short | Modular (de)construction of complex bacterial phenotypes by CRISPR/nCas9-assisted, multiplex cytidine base-editing |
| title_sort | modular de construction of complex bacterial phenotypes by crispr ncas9 assisted multiplex cytidine base editing |
| url | https://doi.org/10.1038/s41467-022-30780-z |
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