Engineering of tenogenic patch scaffold with fibrous microtopography and reinforcement via uniaxial cold-drawing
Rotator cuff tendon repair remains a great challenge in orthopedic surgery. Attempts to inspire the microenvironmental recapitulation from in vivo have rarely elaborated on aligned and reinforced fibrous microtopography required for clinical application. Furthermore, compromises with fibrous densifi...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-05-01
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| Series: | Materials & Design |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0264127522002301 |
| _version_ | 1818232131462103040 |
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| author | Ting Guo Fang Zhong Feng Wen Peng Liu Eng San Thian Hemin Nie Dan Li Rongkai Zhang Chao Ma Nan Lin Zuyong Wang |
| author_facet | Ting Guo Fang Zhong Feng Wen Peng Liu Eng San Thian Hemin Nie Dan Li Rongkai Zhang Chao Ma Nan Lin Zuyong Wang |
| author_sort | Ting Guo |
| collection | DOAJ |
| description | Rotator cuff tendon repair remains a great challenge in orthopedic surgery. Attempts to inspire the microenvironmental recapitulation from in vivo have rarely elaborated on aligned and reinforced fibrous microtopography required for clinical application. Furthermore, compromises with fibrous densification during fabrication often limit the scaffold’s relevance in translational medicine. Here, we reported a facile and scalable method of uniaxial cold-drawing for engineering tendon patch scaffold. Comprised of bioactive and structure-supporting portions, the scaffold obtained featured microtopography of aligned and sparely-stacked fibres while providing mechanical properties approaching to that of human rotator cuff tendon. The structural integration between scaffold’s two portions resulted in stable and reliable fabrication, where parameters of drawing ratio, speed and temperature influenced the formation of fibrous microtopography. When mesenchymal stem cells were cultured with the scaffold, phenotypic markers of tenogenesis including scleraxis, decorin and tenomodulin were examined higher over nonfibrous controls. Our results further demonstrated that expression of collagen type I was associated with highly aligned intracellular cytoskeleton and the higher nucleus alignment and elongation, coupling with down-regulated cell proliferation and noncytotoxicity. |
| first_indexed | 2024-12-12T11:01:25Z |
| format | Article |
| id | doaj.art-0f36bae3dfc74870982824c4af223d0f |
| institution | Directory Open Access Journal |
| issn | 0264-1275 |
| language | English |
| last_indexed | 2024-12-12T11:01:25Z |
| publishDate | 2022-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials & Design |
| spelling | doaj.art-0f36bae3dfc74870982824c4af223d0f2022-12-22T00:26:30ZengElsevierMaterials & Design0264-12752022-05-01217110609Engineering of tenogenic patch scaffold with fibrous microtopography and reinforcement via uniaxial cold-drawingTing Guo0Fang Zhong1Feng Wen2Peng Liu3Eng San Thian4Hemin Nie5Dan Li6Rongkai Zhang7Chao Ma8Nan Lin9Zuyong Wang10College of Materials Science and Engineering, College of Biology, Hunan University, Changsha 410082, PR ChinaCollege of Materials Science and Engineering, College of Biology, Hunan University, Changsha 410082, PR ChinaWenzhou Institute, University of Chinese Academy of Sciences, Wenzhou 325000, PR ChinaCollege of Materials Science and Engineering, College of Biology, Hunan University, Changsha 410082, PR ChinaDepartment of Mechanical Engineering, National University of Singapore, Singapore 117576, SingaporeCollege of Materials Science and Engineering, College of Biology, Hunan University, Changsha 410082, PR ChinaCollege of Materials Science and Engineering, College of Biology, Hunan University, Changsha 410082, PR ChinaDepartment of Joint Surgery, Guangdong Provincial Key Laboratory of Bone and Joint Degeneration Diseases, The Third Affiliated Hospital, Southern Medical University, Guangzhou 510630, PR China; Orthopedic Hospital of Guangdong Province, Guangzhou 510630, PR ChinaCollege of Materials Science and Engineering, College of Biology, Hunan University, Changsha 410082, PR ChinaCollege of Materials Science and Engineering, College of Biology, Hunan University, Changsha 410082, PR ChinaCollege of Materials Science and Engineering, College of Biology, Hunan University, Changsha 410082, PR China; Corresponding author.Rotator cuff tendon repair remains a great challenge in orthopedic surgery. Attempts to inspire the microenvironmental recapitulation from in vivo have rarely elaborated on aligned and reinforced fibrous microtopography required for clinical application. Furthermore, compromises with fibrous densification during fabrication often limit the scaffold’s relevance in translational medicine. Here, we reported a facile and scalable method of uniaxial cold-drawing for engineering tendon patch scaffold. Comprised of bioactive and structure-supporting portions, the scaffold obtained featured microtopography of aligned and sparely-stacked fibres while providing mechanical properties approaching to that of human rotator cuff tendon. The structural integration between scaffold’s two portions resulted in stable and reliable fabrication, where parameters of drawing ratio, speed and temperature influenced the formation of fibrous microtopography. When mesenchymal stem cells were cultured with the scaffold, phenotypic markers of tenogenesis including scleraxis, decorin and tenomodulin were examined higher over nonfibrous controls. Our results further demonstrated that expression of collagen type I was associated with highly aligned intracellular cytoskeleton and the higher nucleus alignment and elongation, coupling with down-regulated cell proliferation and noncytotoxicity.http://www.sciencedirect.com/science/article/pii/S0264127522002301Tissue regenerationTendonScaffoldPoly(epsilon-caprolactone)Electrospinning |
| spellingShingle | Ting Guo Fang Zhong Feng Wen Peng Liu Eng San Thian Hemin Nie Dan Li Rongkai Zhang Chao Ma Nan Lin Zuyong Wang Engineering of tenogenic patch scaffold with fibrous microtopography and reinforcement via uniaxial cold-drawing Materials & Design Tissue regeneration Tendon Scaffold Poly(epsilon-caprolactone) Electrospinning |
| title | Engineering of tenogenic patch scaffold with fibrous microtopography and reinforcement via uniaxial cold-drawing |
| title_full | Engineering of tenogenic patch scaffold with fibrous microtopography and reinforcement via uniaxial cold-drawing |
| title_fullStr | Engineering of tenogenic patch scaffold with fibrous microtopography and reinforcement via uniaxial cold-drawing |
| title_full_unstemmed | Engineering of tenogenic patch scaffold with fibrous microtopography and reinforcement via uniaxial cold-drawing |
| title_short | Engineering of tenogenic patch scaffold with fibrous microtopography and reinforcement via uniaxial cold-drawing |
| title_sort | engineering of tenogenic patch scaffold with fibrous microtopography and reinforcement via uniaxial cold drawing |
| topic | Tissue regeneration Tendon Scaffold Poly(epsilon-caprolactone) Electrospinning |
| url | http://www.sciencedirect.com/science/article/pii/S0264127522002301 |
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