Pesticide Residues and Stuck Fermentation in Wine: New Evidences Indicate the Urgent Need of Tailored Regulations
For three consecutive years, an Italian winery in Apulia has dealt with sudden alcoholic stuck fermentation in the early stages of vinification process, i.e., typical defects addressable to bacterial spoilage. After a prescreening trial, we assessed, for the first time, the influence of the commerci...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-02-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/5/1/23 |
| _version_ | 1828201739829903360 |
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| author | Pasquale Russo Carmen Berbegal Cristina De Ceglie Francesco Grieco Giuseppe Spano Vittorio Capozzi |
| author_facet | Pasquale Russo Carmen Berbegal Cristina De Ceglie Francesco Grieco Giuseppe Spano Vittorio Capozzi |
| author_sort | Pasquale Russo |
| collection | DOAJ |
| description | For three consecutive years, an Italian winery in Apulia has dealt with sudden alcoholic stuck fermentation in the early stages of vinification process, i.e., typical defects addressable to bacterial spoilage. After a prescreening trial, we assessed, for the first time, the influence of the commercial fungicide preparation Ridomil Gold<sup>®</sup> (Combi Pepite), containing Metalaxyl-M (4.85%) and Folpet (40%) as active principles, on the growth of several yeasts (<i>Saccharomyces cerevisiae</i> and non-<i>Saccharomyces</i> spp.) and lactic acid bacteria of oenological interest. We also tested, separately and in combination, the effects of Metalaxyl-M and Folpet molecules on microbial growth both in culture media and in grape must. We recalled the attention on Folpet negative effect on yeasts, extending its inhibitory spectrum on non-<i>Saccharomyces</i> (e.g., <i>Candida</i> spp.). Moreover, we highlighted a synergic effect of Metalaxyl-M and Folpet used together and a possible inhibitory role of the fungicide excipients. Interestingly, we identified the autochthonous <i>S. cerevisiae</i> strain E4 as moderately resistant to the Folpet toxicity. Our findings clearly indicate the urgent need for integrating the screening procedures for admission of pesticides for use on wine grape with trials testing their effects on the physiology of protechnological microbes. |
| first_indexed | 2024-04-12T11:35:04Z |
| format | Article |
| id | doaj.art-0f39b970f8514ad18dd5f2a446efd8b8 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-04-12T11:35:04Z |
| publishDate | 2019-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-0f39b970f8514ad18dd5f2a446efd8b82022-12-22T03:34:51ZengMDPI AGFermentation2311-56372019-02-01512310.3390/fermentation5010023fermentation5010023Pesticide Residues and Stuck Fermentation in Wine: New Evidences Indicate the Urgent Need of Tailored RegulationsPasquale Russo0Carmen Berbegal1Cristina De Ceglie2Francesco Grieco3Giuseppe Spano4Vittorio Capozzi5Dipartimento di Scienze Agrarie, degli Alimenti e dell’Ambiente, Università di Foggia, via Napoli 25, 71100 Foggia, ItalyDipartimento di Scienze Agrarie, degli Alimenti e dell’Ambiente, Università di Foggia, via Napoli 25, 71100 Foggia, ItalyDipartimento di Scienze Agrarie, degli Alimenti e dell’Ambiente, Università di Foggia, via Napoli 25, 71100 Foggia, ItalyIstituto di Scienze delle Produzioni Alimentari, Consiglio Nazionale delle Ricerche, Unità Operativa di Supporto di Lecce, 73100 Lecce, ItalyDipartimento di Scienze Agrarie, degli Alimenti e dell’Ambiente, Università di Foggia, via Napoli 25, 71100 Foggia, ItalyDipartimento di Scienze Agrarie, degli Alimenti e dell’Ambiente, Università di Foggia, via Napoli 25, 71100 Foggia, ItalyFor three consecutive years, an Italian winery in Apulia has dealt with sudden alcoholic stuck fermentation in the early stages of vinification process, i.e., typical defects addressable to bacterial spoilage. After a prescreening trial, we assessed, for the first time, the influence of the commercial fungicide preparation Ridomil Gold<sup>®</sup> (Combi Pepite), containing Metalaxyl-M (4.85%) and Folpet (40%) as active principles, on the growth of several yeasts (<i>Saccharomyces cerevisiae</i> and non-<i>Saccharomyces</i> spp.) and lactic acid bacteria of oenological interest. We also tested, separately and in combination, the effects of Metalaxyl-M and Folpet molecules on microbial growth both in culture media and in grape must. We recalled the attention on Folpet negative effect on yeasts, extending its inhibitory spectrum on non-<i>Saccharomyces</i> (e.g., <i>Candida</i> spp.). Moreover, we highlighted a synergic effect of Metalaxyl-M and Folpet used together and a possible inhibitory role of the fungicide excipients. Interestingly, we identified the autochthonous <i>S. cerevisiae</i> strain E4 as moderately resistant to the Folpet toxicity. Our findings clearly indicate the urgent need for integrating the screening procedures for admission of pesticides for use on wine grape with trials testing their effects on the physiology of protechnological microbes.https://www.mdpi.com/2311-5637/5/1/23pesticidefungicidewinealcoholic fermentationyeaststuck fermentation |
| spellingShingle | Pasquale Russo Carmen Berbegal Cristina De Ceglie Francesco Grieco Giuseppe Spano Vittorio Capozzi Pesticide Residues and Stuck Fermentation in Wine: New Evidences Indicate the Urgent Need of Tailored Regulations Fermentation pesticide fungicide wine alcoholic fermentation yeast stuck fermentation |
| title | Pesticide Residues and Stuck Fermentation in Wine: New Evidences Indicate the Urgent Need of Tailored Regulations |
| title_full | Pesticide Residues and Stuck Fermentation in Wine: New Evidences Indicate the Urgent Need of Tailored Regulations |
| title_fullStr | Pesticide Residues and Stuck Fermentation in Wine: New Evidences Indicate the Urgent Need of Tailored Regulations |
| title_full_unstemmed | Pesticide Residues and Stuck Fermentation in Wine: New Evidences Indicate the Urgent Need of Tailored Regulations |
| title_short | Pesticide Residues and Stuck Fermentation in Wine: New Evidences Indicate the Urgent Need of Tailored Regulations |
| title_sort | pesticide residues and stuck fermentation in wine new evidences indicate the urgent need of tailored regulations |
| topic | pesticide fungicide wine alcoholic fermentation yeast stuck fermentation |
| url | https://www.mdpi.com/2311-5637/5/1/23 |
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