Nanoscale to Macroscale Characterization of in—Situ Bacterial Biopolymers for Applications in Soil Stabilization
Bacterial biopolymers produced extracellularly due to microbial metabolic activities have gained considerable interest in various engineering applications. The major advantages of bacterial biopolymers is their in-situ production and low water solubility, eliminating the requirement for mixing in gr...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-01-01
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| Series: | Frontiers in Materials |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmats.2021.681850/full |
| _version_ | 1818751604063469568 |
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| author | Asha Latha Ramachandran Abhijit Mukherjee Navdeep K Dhami |
| author_facet | Asha Latha Ramachandran Abhijit Mukherjee Navdeep K Dhami |
| author_sort | Asha Latha Ramachandran |
| collection | DOAJ |
| description | Bacterial biopolymers produced extracellularly due to microbial metabolic activities have gained considerable interest in various engineering applications. The major advantages of bacterial biopolymers is their in-situ production and low water solubility, eliminating the requirement for mixing in granular substrates such as soils. These properties make them highly desirable and preferable to manufactured biopolymers. But for any engineering applications, it is crucial to understand the mechanical properties of these materials, which have been less explored. This investigation is the first attempt to quantify the nano and macro mechanical properties of in-situ bacterial biopolymer dextran produced by bacterial culture Leucononstoc mesenteroids. The fundamental mechanism of bacterial biopolymer-based cementation has been revealed through their morphographic and nanomechanical testing via atomic force microscopy, nanoindentation and scanning electron micrographs. The effect of bacterially produced biopolymers and commercial biopolymers on the macro-mechanical properties of soils was then investigated via needle penetration tests. In-situ biopolymers were found to be highly effective in stabilizing soils with comparable mechanical properties as commercial biopolymers. This study has demonstrated novel methods for testing in situ polymers and opened up the channels for their applications in numerous subsurface as well as surface applications. |
| first_indexed | 2024-12-18T04:38:12Z |
| format | Article |
| id | doaj.art-0f39f2cd05d54d8d80ca7dee51a3d15c |
| institution | Directory Open Access Journal |
| issn | 2296-8016 |
| language | English |
| last_indexed | 2024-12-18T04:38:12Z |
| publishDate | 2022-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Materials |
| spelling | doaj.art-0f39f2cd05d54d8d80ca7dee51a3d15c2022-12-21T21:20:47ZengFrontiers Media S.A.Frontiers in Materials2296-80162022-01-01810.3389/fmats.2021.681850681850Nanoscale to Macroscale Characterization of in—Situ Bacterial Biopolymers for Applications in Soil StabilizationAsha Latha RamachandranAbhijit MukherjeeNavdeep K DhamiBacterial biopolymers produced extracellularly due to microbial metabolic activities have gained considerable interest in various engineering applications. The major advantages of bacterial biopolymers is their in-situ production and low water solubility, eliminating the requirement for mixing in granular substrates such as soils. These properties make them highly desirable and preferable to manufactured biopolymers. But for any engineering applications, it is crucial to understand the mechanical properties of these materials, which have been less explored. This investigation is the first attempt to quantify the nano and macro mechanical properties of in-situ bacterial biopolymer dextran produced by bacterial culture Leucononstoc mesenteroids. The fundamental mechanism of bacterial biopolymer-based cementation has been revealed through their morphographic and nanomechanical testing via atomic force microscopy, nanoindentation and scanning electron micrographs. The effect of bacterially produced biopolymers and commercial biopolymers on the macro-mechanical properties of soils was then investigated via needle penetration tests. In-situ biopolymers were found to be highly effective in stabilizing soils with comparable mechanical properties as commercial biopolymers. This study has demonstrated novel methods for testing in situ polymers and opened up the channels for their applications in numerous subsurface as well as surface applications.https://www.frontiersin.org/articles/10.3389/fmats.2021.681850/fullbiopolymersbacterial dextrannanoindentationAFMneedle penetration |
| spellingShingle | Asha Latha Ramachandran Abhijit Mukherjee Navdeep K Dhami Nanoscale to Macroscale Characterization of in—Situ Bacterial Biopolymers for Applications in Soil Stabilization Frontiers in Materials biopolymers bacterial dextran nanoindentation AFM needle penetration |
| title | Nanoscale to Macroscale Characterization of in—Situ Bacterial Biopolymers for Applications in Soil Stabilization |
| title_full | Nanoscale to Macroscale Characterization of in—Situ Bacterial Biopolymers for Applications in Soil Stabilization |
| title_fullStr | Nanoscale to Macroscale Characterization of in—Situ Bacterial Biopolymers for Applications in Soil Stabilization |
| title_full_unstemmed | Nanoscale to Macroscale Characterization of in—Situ Bacterial Biopolymers for Applications in Soil Stabilization |
| title_short | Nanoscale to Macroscale Characterization of in—Situ Bacterial Biopolymers for Applications in Soil Stabilization |
| title_sort | nanoscale to macroscale characterization of in situ bacterial biopolymers for applications in soil stabilization |
| topic | biopolymers bacterial dextran nanoindentation AFM needle penetration |
| url | https://www.frontiersin.org/articles/10.3389/fmats.2021.681850/full |
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