Contrasted patterns of crossover and non-crossover at Arabidopsis thaliana meiotic recombination hotspots.
The vast majority of meiotic recombination events (crossovers (COs) and non-crossovers (NCOs)) cluster in narrow hotspots surrounded by large regions devoid of recombinational activity. Here, using a new molecular approach in plants, called "pollen-typing", we detected and characterized hu...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-11-01
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| Series: | PLoS Genetics |
| Online Access: | http://europepmc.org/articles/PMC3828143?pdf=render |
| _version_ | 1818128907621105664 |
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| author | Jan Drouaud Hossein Khademian Laurène Giraut Vanessa Zanni Sarah Bellalou Ian R Henderson Matthieu Falque Christine Mézard |
| author_facet | Jan Drouaud Hossein Khademian Laurène Giraut Vanessa Zanni Sarah Bellalou Ian R Henderson Matthieu Falque Christine Mézard |
| author_sort | Jan Drouaud |
| collection | DOAJ |
| description | The vast majority of meiotic recombination events (crossovers (COs) and non-crossovers (NCOs)) cluster in narrow hotspots surrounded by large regions devoid of recombinational activity. Here, using a new molecular approach in plants, called "pollen-typing", we detected and characterized hundreds of CO and NCO molecules in two different hotspot regions in Arabidopsis thaliana. This analysis revealed that COs are concentrated in regions of a few kilobases where their rates reach up to 50 times the genome average. The hotspots themselves tend to cluster in regions less than 8 kilobases in size with overlapping CO distribution. Non-crossover (NCO) events also occurred in the two hotspots but at very different levels (local CO/NCO ratios of 1/1 and 30/1) and their track lengths were quite small (a few hundred base pairs). We also showed that the ZMM protein MSH4 plays a role in CO formation and somewhat unexpectedly we also found that it is involved in the generation of NCOs but with a different level of effect. Finally, factors acting in cis and in trans appear to shape the rate and distribution of COs at meiotic recombination hotspots. |
| first_indexed | 2024-12-11T07:40:43Z |
| format | Article |
| id | doaj.art-0f3bb144c0224612b10aafd213837f5f |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-12-11T07:40:43Z |
| publishDate | 2013-11-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-0f3bb144c0224612b10aafd213837f5f2022-12-22T01:15:35ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042013-11-01911e100392210.1371/journal.pgen.1003922Contrasted patterns of crossover and non-crossover at Arabidopsis thaliana meiotic recombination hotspots.Jan DrouaudHossein KhademianLaurène GirautVanessa ZanniSarah BellalouIan R HendersonMatthieu FalqueChristine MézardThe vast majority of meiotic recombination events (crossovers (COs) and non-crossovers (NCOs)) cluster in narrow hotspots surrounded by large regions devoid of recombinational activity. Here, using a new molecular approach in plants, called "pollen-typing", we detected and characterized hundreds of CO and NCO molecules in two different hotspot regions in Arabidopsis thaliana. This analysis revealed that COs are concentrated in regions of a few kilobases where their rates reach up to 50 times the genome average. The hotspots themselves tend to cluster in regions less than 8 kilobases in size with overlapping CO distribution. Non-crossover (NCO) events also occurred in the two hotspots but at very different levels (local CO/NCO ratios of 1/1 and 30/1) and their track lengths were quite small (a few hundred base pairs). We also showed that the ZMM protein MSH4 plays a role in CO formation and somewhat unexpectedly we also found that it is involved in the generation of NCOs but with a different level of effect. Finally, factors acting in cis and in trans appear to shape the rate and distribution of COs at meiotic recombination hotspots.http://europepmc.org/articles/PMC3828143?pdf=render |
| spellingShingle | Jan Drouaud Hossein Khademian Laurène Giraut Vanessa Zanni Sarah Bellalou Ian R Henderson Matthieu Falque Christine Mézard Contrasted patterns of crossover and non-crossover at Arabidopsis thaliana meiotic recombination hotspots. PLoS Genetics |
| title | Contrasted patterns of crossover and non-crossover at Arabidopsis thaliana meiotic recombination hotspots. |
| title_full | Contrasted patterns of crossover and non-crossover at Arabidopsis thaliana meiotic recombination hotspots. |
| title_fullStr | Contrasted patterns of crossover and non-crossover at Arabidopsis thaliana meiotic recombination hotspots. |
| title_full_unstemmed | Contrasted patterns of crossover and non-crossover at Arabidopsis thaliana meiotic recombination hotspots. |
| title_short | Contrasted patterns of crossover and non-crossover at Arabidopsis thaliana meiotic recombination hotspots. |
| title_sort | contrasted patterns of crossover and non crossover at arabidopsis thaliana meiotic recombination hotspots |
| url | http://europepmc.org/articles/PMC3828143?pdf=render |
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