Proteomics of Two Thermotolerant Isolates of <i>Trichoderma</i> under High-Temperature Stress
Several species of the soil borne fungus of the genus <i>Trichoderma</i> are known to be versatile, opportunistic plant symbionts and are the most successful biocontrol agents used in today’s agriculture. To be successful in field conditions, the fungus must endure varying climatic condi...
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| Format: | Article |
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MDPI AG
2021-11-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/7/12/1002 |
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| author | Sowmya Poosapati Prasad Durga Ravulapalli Dinesh Kumar Viswanathaswamy Monica Kannan |
| author_facet | Sowmya Poosapati Prasad Durga Ravulapalli Dinesh Kumar Viswanathaswamy Monica Kannan |
| author_sort | Sowmya Poosapati |
| collection | DOAJ |
| description | Several species of the soil borne fungus of the genus <i>Trichoderma</i> are known to be versatile, opportunistic plant symbionts and are the most successful biocontrol agents used in today’s agriculture. To be successful in field conditions, the fungus must endure varying climatic conditions. Studies have indicated that a high atmospheric temperature coupled with low humidity is a major factor in the inconsistent performance of <i>Trichoderma</i> under field conditions. Understanding the molecular modulations associated with <i>Trichoderma</i> that persist and deliver under abiotic stress conditions will aid in exploiting the value of these organisms for such uses. In this study, a comparative proteomic analysis, using two-dimensional gel electrophoresis (2DE) and matrix-assisted laser desorption/time-of-flight (MALDI-TOF-TOF) mass spectrometry, was used to identify proteins associated with thermotolerance in two thermotolerant isolates of <i>Trichoderma</i>: <i>T. longibrachiatum</i> 673, TaDOR673 and <i>T. asperellum</i> 7316, TaDOR7316; with 32 differentially expressed proteins being identified. Sequence homology and conserved domains were used to identify these proteins and to assign a probable function to them. The thermotolerant isolate, TaDOR673, seemed to employ the stress signaling MAPK pathways and heat shock response pathways to combat the stress condition, whereas the moderately tolerant isolate, TaDOR7316, seemed to adapt to high-temperature conditions by reducing the accumulation of misfolded proteins through an unfolded protein response pathway and autophagy. In addition, there were unique, as well as common, proteins that were differentially expressed in the two isolates studied. |
| first_indexed | 2024-03-10T03:47:30Z |
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| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-10T03:47:30Z |
| publishDate | 2021-11-01 |
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| series | Journal of Fungi |
| spelling | doaj.art-0f3f2864f30c4f779e2b03d0a4f8d3202023-11-23T09:04:26ZengMDPI AGJournal of Fungi2309-608X2021-11-01712100210.3390/jof7121002Proteomics of Two Thermotolerant Isolates of <i>Trichoderma</i> under High-Temperature StressSowmya Poosapati0Prasad Durga Ravulapalli1Dinesh Kumar Viswanathaswamy2Monica Kannan3Department of Plant Pathology, ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad 500030, IndiaDepartment of Plant Pathology, ICAR-Indian Institute of Oilseeds Research, Rajendranagar, Hyderabad 500030, IndiaDepartment of Biotechnology, ICAR-Indian Institute of Oilseeds Research Rajendranagar, Hyderabad 500030, IndiaProteomics Facility, School of Life Sciences, University of Hyderabad, Gachibowli, Hyderabad 500046, IndiaSeveral species of the soil borne fungus of the genus <i>Trichoderma</i> are known to be versatile, opportunistic plant symbionts and are the most successful biocontrol agents used in today’s agriculture. To be successful in field conditions, the fungus must endure varying climatic conditions. Studies have indicated that a high atmospheric temperature coupled with low humidity is a major factor in the inconsistent performance of <i>Trichoderma</i> under field conditions. Understanding the molecular modulations associated with <i>Trichoderma</i> that persist and deliver under abiotic stress conditions will aid in exploiting the value of these organisms for such uses. In this study, a comparative proteomic analysis, using two-dimensional gel electrophoresis (2DE) and matrix-assisted laser desorption/time-of-flight (MALDI-TOF-TOF) mass spectrometry, was used to identify proteins associated with thermotolerance in two thermotolerant isolates of <i>Trichoderma</i>: <i>T. longibrachiatum</i> 673, TaDOR673 and <i>T. asperellum</i> 7316, TaDOR7316; with 32 differentially expressed proteins being identified. Sequence homology and conserved domains were used to identify these proteins and to assign a probable function to them. The thermotolerant isolate, TaDOR673, seemed to employ the stress signaling MAPK pathways and heat shock response pathways to combat the stress condition, whereas the moderately tolerant isolate, TaDOR7316, seemed to adapt to high-temperature conditions by reducing the accumulation of misfolded proteins through an unfolded protein response pathway and autophagy. In addition, there were unique, as well as common, proteins that were differentially expressed in the two isolates studied.https://www.mdpi.com/2309-608X/7/12/1002<i>Trichoderma</i>thermotolerancecell wall remodelingMAPKHsf1UPR |
| spellingShingle | Sowmya Poosapati Prasad Durga Ravulapalli Dinesh Kumar Viswanathaswamy Monica Kannan Proteomics of Two Thermotolerant Isolates of <i>Trichoderma</i> under High-Temperature Stress Journal of Fungi <i>Trichoderma</i> thermotolerance cell wall remodeling MAPK Hsf1 UPR |
| title | Proteomics of Two Thermotolerant Isolates of <i>Trichoderma</i> under High-Temperature Stress |
| title_full | Proteomics of Two Thermotolerant Isolates of <i>Trichoderma</i> under High-Temperature Stress |
| title_fullStr | Proteomics of Two Thermotolerant Isolates of <i>Trichoderma</i> under High-Temperature Stress |
| title_full_unstemmed | Proteomics of Two Thermotolerant Isolates of <i>Trichoderma</i> under High-Temperature Stress |
| title_short | Proteomics of Two Thermotolerant Isolates of <i>Trichoderma</i> under High-Temperature Stress |
| title_sort | proteomics of two thermotolerant isolates of i trichoderma i under high temperature stress |
| topic | <i>Trichoderma</i> thermotolerance cell wall remodeling MAPK Hsf1 UPR |
| url | https://www.mdpi.com/2309-608X/7/12/1002 |
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