Chitosan as an Alternative to Oil-Based Materials for the Fabrication of Lab-on-a-Chip
Given the growing importance of lab-on-a-chip in a number of fields, such as medical diagnosis or environmental analysis, the fact that the current fabrication process relies mainly on oil-based polymers raises an ecological concern. As an eco-responsible alternative, we presented, in this article,...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-03-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/15/3/379 |
| _version_ | 1827305552207675392 |
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| author | Morgane Zimmer Stéphane Trombotto Emmanuelle Laurenceau Anne-Laure Deman |
| author_facet | Morgane Zimmer Stéphane Trombotto Emmanuelle Laurenceau Anne-Laure Deman |
| author_sort | Morgane Zimmer |
| collection | DOAJ |
| description | Given the growing importance of lab-on-a-chip in a number of fields, such as medical diagnosis or environmental analysis, the fact that the current fabrication process relies mainly on oil-based polymers raises an ecological concern. As an eco-responsible alternative, we presented, in this article, a manufacturing process for microfluidic devices from chitosan, a bio-sourced, biodegradable, and biocompatible polysaccharide. From chitosan powder, we produced thick and rigid films. To prevent their dissolution and reduce their swelling when in contact with aqueous solutions, we investigated a film neutralization step and characterized the mechanical and physical properties of the resulting films. On these neutralized chitosan films, we compared two micropatterning methods, i.e., hot embossing and mechanical micro-drilling, based on the resolution of microchannels from 100 µm to 1000 µm wide. Then, chitosan films with micro-drilled channels were bonded using a biocompatible dry photoresist on a glass slide or another neutralized chitosan film. Thanks to this protocol, the first functional chitosan microfluidic devices were prepared. While some steps of the fabrication process remain to be improved, these preliminary results pave the way toward a sustainable fabrication of lab-on-a-chip. |
| first_indexed | 2024-04-24T18:00:03Z |
| format | Article |
| id | doaj.art-0f3f9946543d4c109c0605694177fbe4 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-04-24T18:00:03Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-0f3f9946543d4c109c0605694177fbe42024-03-27T13:55:14ZengMDPI AGMicromachines2072-666X2024-03-0115337910.3390/mi15030379Chitosan as an Alternative to Oil-Based Materials for the Fabrication of Lab-on-a-ChipMorgane Zimmer0Stéphane Trombotto1Emmanuelle Laurenceau2Anne-Laure Deman3Ecole Centrale de Lyon, INSA Lyon, CNRS, Université Claude Bernard Lyon 1, CPE Lyon, INL, UMR5270, 69130 Ecully, FranceIngénierie des Matériaux Polymères (UMR5223), Université Claude Bernard Lyon 1, CNRS, INSA Lyon, Université Jean Monnet, 69622 Villeurbanne, FranceEcole Centrale de Lyon, INSA Lyon, CNRS, Université Claude Bernard Lyon 1, CPE Lyon, INL, UMR5270, 69130 Ecully, FranceEcole Centrale de Lyon, INSA Lyon, CNRS, Université Claude Bernard Lyon 1, CPE Lyon, INL, UMR5270, 69130 Ecully, FranceGiven the growing importance of lab-on-a-chip in a number of fields, such as medical diagnosis or environmental analysis, the fact that the current fabrication process relies mainly on oil-based polymers raises an ecological concern. As an eco-responsible alternative, we presented, in this article, a manufacturing process for microfluidic devices from chitosan, a bio-sourced, biodegradable, and biocompatible polysaccharide. From chitosan powder, we produced thick and rigid films. To prevent their dissolution and reduce their swelling when in contact with aqueous solutions, we investigated a film neutralization step and characterized the mechanical and physical properties of the resulting films. On these neutralized chitosan films, we compared two micropatterning methods, i.e., hot embossing and mechanical micro-drilling, based on the resolution of microchannels from 100 µm to 1000 µm wide. Then, chitosan films with micro-drilled channels were bonded using a biocompatible dry photoresist on a glass slide or another neutralized chitosan film. Thanks to this protocol, the first functional chitosan microfluidic devices were prepared. While some steps of the fabrication process remain to be improved, these preliminary results pave the way toward a sustainable fabrication of lab-on-a-chip.https://www.mdpi.com/2072-666X/15/3/379chitosanlab-on-a-chipmicrofluidicbiomaterialsmicro-drillinghot embossing |
| spellingShingle | Morgane Zimmer Stéphane Trombotto Emmanuelle Laurenceau Anne-Laure Deman Chitosan as an Alternative to Oil-Based Materials for the Fabrication of Lab-on-a-Chip Micromachines chitosan lab-on-a-chip microfluidic biomaterials micro-drilling hot embossing |
| title | Chitosan as an Alternative to Oil-Based Materials for the Fabrication of Lab-on-a-Chip |
| title_full | Chitosan as an Alternative to Oil-Based Materials for the Fabrication of Lab-on-a-Chip |
| title_fullStr | Chitosan as an Alternative to Oil-Based Materials for the Fabrication of Lab-on-a-Chip |
| title_full_unstemmed | Chitosan as an Alternative to Oil-Based Materials for the Fabrication of Lab-on-a-Chip |
| title_short | Chitosan as an Alternative to Oil-Based Materials for the Fabrication of Lab-on-a-Chip |
| title_sort | chitosan as an alternative to oil based materials for the fabrication of lab on a chip |
| topic | chitosan lab-on-a-chip microfluidic biomaterials micro-drilling hot embossing |
| url | https://www.mdpi.com/2072-666X/15/3/379 |
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