Simultaneous manipulation of lignin structure and secondary cell wall formation in transgenic poplar
Abstract Increasing the wood density to a preferred range contributes to upgrading the value of wood as raw material. Lignin manipulation can also improve wood quality. This study attempted to accelerate secondary cell wall formation in transgenic poplar with an altered lignin structure. To achieve...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2020-08-01
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| Series: | Journal of Wood Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s10086-020-01902-2 |
| _version_ | 1819152063067586560 |
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| author | Nabuqi Nuoendagula Sejirihu Wu Naoki Takata Shingo Sakamoto Masanobu Yamamoto Mikiko Uesugi Annabelle Déjardin Gilles Pilate Toru Taniguchi Nobutaka Mitsuda Shinya Kajita |
| author_facet | Nabuqi Nuoendagula Sejirihu Wu Naoki Takata Shingo Sakamoto Masanobu Yamamoto Mikiko Uesugi Annabelle Déjardin Gilles Pilate Toru Taniguchi Nobutaka Mitsuda Shinya Kajita |
| author_sort | Nabuqi |
| collection | DOAJ |
| description | Abstract Increasing the wood density to a preferred range contributes to upgrading the value of wood as raw material. Lignin manipulation can also improve wood quality. This study attempted to accelerate secondary cell wall formation in transgenic poplar with an altered lignin structure. To achieve this, OsSWN1, a rice master switch for secondary cell wall formation in fiber cells, was overexpressed in poplar plants in which monolignol biosynthesis was suppressed by the expression of an RNA-interference construct targeted to the gene for cinnamyl alcohol dehydrogenase 1. The generated transgenic poplars successfully overexpressed the chimeric OsSWN1 construct and monolignol biosynthesis remained altered in these plants. Secondary cell wall thickness was increased in the transgenic plants and their wood density was higher compared to the background line. No difference in lignin content was observed, except in one transgenic line. High saccharification characteristics observed in the background line were preserved in the transgenic line with lower OsSWN1 overexpression, but higher OsSWN1 overexpression had a slight negative impact on enzyme saccharification. Our data suggested that fine-tuning of accelerated deposition of the secondary cell wall combined with alteration of monolignol biosynthesis should improve the lignocellulose quality for conventional and future biorefinery uses. |
| first_indexed | 2024-12-22T14:43:20Z |
| format | Article |
| id | doaj.art-0f470100f6764a399634804cf68ee3b2 |
| institution | Directory Open Access Journal |
| issn | 1435-0211 1611-4663 |
| language | English |
| last_indexed | 2024-12-22T14:43:20Z |
| publishDate | 2020-08-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Journal of Wood Science |
| spelling | doaj.art-0f470100f6764a399634804cf68ee3b22022-12-21T18:22:30ZengSpringerOpenJournal of Wood Science1435-02111611-46632020-08-016611910.1186/s10086-020-01902-2Simultaneous manipulation of lignin structure and secondary cell wall formation in transgenic poplarNabuqi0Nuoendagula1Sejirihu Wu2Naoki Takata3Shingo Sakamoto4Masanobu Yamamoto5Mikiko Uesugi6Annabelle Déjardin7Gilles Pilate8Toru Taniguchi9Nobutaka Mitsuda10Shinya Kajita11Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and TechnologyGraduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and TechnologyGraduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and TechnologyForest Bio-Research Center, Forestry and Forest Products Research Institute, Forest Research and Management OrganizationBioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and TechnologyGraduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and TechnologyINRAE, ONF, BioForAINRAE, ONF, BioForAForest Bio-Research Center, Forestry and Forest Products Research Institute, Forest Research and Management OrganizationBioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and TechnologyAbstract Increasing the wood density to a preferred range contributes to upgrading the value of wood as raw material. Lignin manipulation can also improve wood quality. This study attempted to accelerate secondary cell wall formation in transgenic poplar with an altered lignin structure. To achieve this, OsSWN1, a rice master switch for secondary cell wall formation in fiber cells, was overexpressed in poplar plants in which monolignol biosynthesis was suppressed by the expression of an RNA-interference construct targeted to the gene for cinnamyl alcohol dehydrogenase 1. The generated transgenic poplars successfully overexpressed the chimeric OsSWN1 construct and monolignol biosynthesis remained altered in these plants. Secondary cell wall thickness was increased in the transgenic plants and their wood density was higher compared to the background line. No difference in lignin content was observed, except in one transgenic line. High saccharification characteristics observed in the background line were preserved in the transgenic line with lower OsSWN1 overexpression, but higher OsSWN1 overexpression had a slight negative impact on enzyme saccharification. Our data suggested that fine-tuning of accelerated deposition of the secondary cell wall combined with alteration of monolignol biosynthesis should improve the lignocellulose quality for conventional and future biorefinery uses.https://doi.org/10.1186/s10086-020-01902-2Genetic engineeringPoplarTranscription factorWood densityXylem |
| spellingShingle | Nabuqi Nuoendagula Sejirihu Wu Naoki Takata Shingo Sakamoto Masanobu Yamamoto Mikiko Uesugi Annabelle Déjardin Gilles Pilate Toru Taniguchi Nobutaka Mitsuda Shinya Kajita Simultaneous manipulation of lignin structure and secondary cell wall formation in transgenic poplar Journal of Wood Science Genetic engineering Poplar Transcription factor Wood density Xylem |
| title | Simultaneous manipulation of lignin structure and secondary cell wall formation in transgenic poplar |
| title_full | Simultaneous manipulation of lignin structure and secondary cell wall formation in transgenic poplar |
| title_fullStr | Simultaneous manipulation of lignin structure and secondary cell wall formation in transgenic poplar |
| title_full_unstemmed | Simultaneous manipulation of lignin structure and secondary cell wall formation in transgenic poplar |
| title_short | Simultaneous manipulation of lignin structure and secondary cell wall formation in transgenic poplar |
| title_sort | simultaneous manipulation of lignin structure and secondary cell wall formation in transgenic poplar |
| topic | Genetic engineering Poplar Transcription factor Wood density Xylem |
| url | https://doi.org/10.1186/s10086-020-01902-2 |
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