Multidose Hyaluronidase Administration as an Optimal Procedure to Degrade Resilient Hyaluronic Acid Soft Tissue Fillers
Minimally invasive hyaluronan (HA) tissue fillers are routinely employed to provide tissue projection and correct age-related skin depressions. HA fillers can advantageously be degraded by hyaluronidase (HAase) administration in case of adverse events. However, clear guidelines regarding the optimal...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/3/1003 |
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| author | Killian Flégeau Jing Jing Romain Brusini Mélanie Gallet Capucine Moreno Lee Walker François Bourdon Jimmy Faivre |
| author_facet | Killian Flégeau Jing Jing Romain Brusini Mélanie Gallet Capucine Moreno Lee Walker François Bourdon Jimmy Faivre |
| author_sort | Killian Flégeau |
| collection | DOAJ |
| description | Minimally invasive hyaluronan (HA) tissue fillers are routinely employed to provide tissue projection and correct age-related skin depressions. HA fillers can advantageously be degraded by hyaluronidase (HAase) administration in case of adverse events. However, clear guidelines regarding the optimal dosage and mode of administration of HAase are missing, leaving a scientific gap for practitioners in their daily practice. In this study, we implemented a novel rheological procedure to rationally evaluate soft tissue filler degradability and optimize their degradation kinetics. TEOSYAL RHA<sup>®</sup> filler degradation kinetics in contact with HAase was monitored in real-time by rheological time sweeps. Gels were shown to degrade as a function of enzymatic activity, HA concentration, and BDDE content, with a concomitant loss of their viscoelastic properties. We further demonstrated that repeated administration of small HAase doses improved HA degradation kinetics over large single doses. Mathematical analyses were developed to evaluate the degradation potential of an enzyme. Finally, we tuned the optimal time between injections and number of enzymatic units, maximizing degradation kinetics. In this study, we have established a scientific rationale for the degradation of HA fillers by multidose HAase administration that could serve as a basis for future clinical management of adverse events. |
| first_indexed | 2024-03-11T09:33:01Z |
| format | Article |
| id | doaj.art-0f4d792e98ec43af8bc076e8a842e06f |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T09:33:01Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-0f4d792e98ec43af8bc076e8a842e06f2023-11-16T17:26:18ZengMDPI AGMolecules1420-30492023-01-01283100310.3390/molecules28031003Multidose Hyaluronidase Administration as an Optimal Procedure to Degrade Resilient Hyaluronic Acid Soft Tissue FillersKillian Flégeau0Jing Jing1Romain Brusini2Mélanie Gallet3Capucine Moreno4Lee Walker5François Bourdon6Jimmy Faivre7Research and Development Department, Teoxane SA, Rue de Lyon 105, 1203 Geneva, SwitzerlandResearch and Development Department, Teoxane SA, Rue de Lyon 105, 1203 Geneva, SwitzerlandResearch and Development Department, Teoxane SA, Rue de Lyon 105, 1203 Geneva, SwitzerlandResearch and Development Department, Teoxane SA, Rue de Lyon 105, 1203 Geneva, SwitzerlandResearch and Development Department, Teoxane SA, Rue de Lyon 105, 1203 Geneva, SwitzerlandPrivate Practice, B City Clinic, 88 Rodney Street, Liverpool L1 9AR, UKResearch and Development Department, Teoxane SA, Rue de Lyon 105, 1203 Geneva, SwitzerlandResearch and Development Department, Teoxane SA, Rue de Lyon 105, 1203 Geneva, SwitzerlandMinimally invasive hyaluronan (HA) tissue fillers are routinely employed to provide tissue projection and correct age-related skin depressions. HA fillers can advantageously be degraded by hyaluronidase (HAase) administration in case of adverse events. However, clear guidelines regarding the optimal dosage and mode of administration of HAase are missing, leaving a scientific gap for practitioners in their daily practice. In this study, we implemented a novel rheological procedure to rationally evaluate soft tissue filler degradability and optimize their degradation kinetics. TEOSYAL RHA<sup>®</sup> filler degradation kinetics in contact with HAase was monitored in real-time by rheological time sweeps. Gels were shown to degrade as a function of enzymatic activity, HA concentration, and BDDE content, with a concomitant loss of their viscoelastic properties. We further demonstrated that repeated administration of small HAase doses improved HA degradation kinetics over large single doses. Mathematical analyses were developed to evaluate the degradation potential of an enzyme. Finally, we tuned the optimal time between injections and number of enzymatic units, maximizing degradation kinetics. In this study, we have established a scientific rationale for the degradation of HA fillers by multidose HAase administration that could serve as a basis for future clinical management of adverse events.https://www.mdpi.com/1420-3049/28/3/1003hyaluronic acidhyaluronidasesoft tissue fillerenzymatic degradationrheology |
| spellingShingle | Killian Flégeau Jing Jing Romain Brusini Mélanie Gallet Capucine Moreno Lee Walker François Bourdon Jimmy Faivre Multidose Hyaluronidase Administration as an Optimal Procedure to Degrade Resilient Hyaluronic Acid Soft Tissue Fillers Molecules hyaluronic acid hyaluronidase soft tissue filler enzymatic degradation rheology |
| title | Multidose Hyaluronidase Administration as an Optimal Procedure to Degrade Resilient Hyaluronic Acid Soft Tissue Fillers |
| title_full | Multidose Hyaluronidase Administration as an Optimal Procedure to Degrade Resilient Hyaluronic Acid Soft Tissue Fillers |
| title_fullStr | Multidose Hyaluronidase Administration as an Optimal Procedure to Degrade Resilient Hyaluronic Acid Soft Tissue Fillers |
| title_full_unstemmed | Multidose Hyaluronidase Administration as an Optimal Procedure to Degrade Resilient Hyaluronic Acid Soft Tissue Fillers |
| title_short | Multidose Hyaluronidase Administration as an Optimal Procedure to Degrade Resilient Hyaluronic Acid Soft Tissue Fillers |
| title_sort | multidose hyaluronidase administration as an optimal procedure to degrade resilient hyaluronic acid soft tissue fillers |
| topic | hyaluronic acid hyaluronidase soft tissue filler enzymatic degradation rheology |
| url | https://www.mdpi.com/1420-3049/28/3/1003 |
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