Preparation of isolated guard cells, containing cell walls, from Vicia faba.
Stomatal movement, initiated by specialized epidermal cells known as guard cells (GCs), plays a pivotal role in plant gas exchange and water use efficiency. Despite protocols existing for isolating GCs through proplasting for carrying out biochemical, physiological, and molecular studies, protocals...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2024-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0299810 |
| _version_ | 1797242862992097280 |
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| author | Sara K Fleetwood Maya Kleiman E Johan Foster |
| author_facet | Sara K Fleetwood Maya Kleiman E Johan Foster |
| author_sort | Sara K Fleetwood |
| collection | DOAJ |
| description | Stomatal movement, initiated by specialized epidermal cells known as guard cells (GCs), plays a pivotal role in plant gas exchange and water use efficiency. Despite protocols existing for isolating GCs through proplasting for carrying out biochemical, physiological, and molecular studies, protocals for isolating GCs with their cell walls still intact have been lacking in the literature. In this paper, we introduce a method for the isolation of complete GCs from Vicia faba and show their membrane to remain impermeable through propidium iodide staining. This methodology enables further in-depth analyses into the cell wall composition of GCs, facilitating our understanding of structure-function relationship governing reversible actuation within cells. |
| first_indexed | 2024-04-24T18:45:58Z |
| format | Article |
| id | doaj.art-0f4db6d6a67b4d7eb588b6b43130d0e6 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-24T18:45:58Z |
| publishDate | 2024-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-0f4db6d6a67b4d7eb588b6b43130d0e62024-03-27T05:32:35ZengPublic Library of Science (PLoS)PLoS ONE1932-62032024-01-01193e029981010.1371/journal.pone.0299810Preparation of isolated guard cells, containing cell walls, from Vicia faba.Sara K FleetwoodMaya KleimanE Johan FosterStomatal movement, initiated by specialized epidermal cells known as guard cells (GCs), plays a pivotal role in plant gas exchange and water use efficiency. Despite protocols existing for isolating GCs through proplasting for carrying out biochemical, physiological, and molecular studies, protocals for isolating GCs with their cell walls still intact have been lacking in the literature. In this paper, we introduce a method for the isolation of complete GCs from Vicia faba and show their membrane to remain impermeable through propidium iodide staining. This methodology enables further in-depth analyses into the cell wall composition of GCs, facilitating our understanding of structure-function relationship governing reversible actuation within cells.https://doi.org/10.1371/journal.pone.0299810 |
| spellingShingle | Sara K Fleetwood Maya Kleiman E Johan Foster Preparation of isolated guard cells, containing cell walls, from Vicia faba. PLoS ONE |
| title | Preparation of isolated guard cells, containing cell walls, from Vicia faba. |
| title_full | Preparation of isolated guard cells, containing cell walls, from Vicia faba. |
| title_fullStr | Preparation of isolated guard cells, containing cell walls, from Vicia faba. |
| title_full_unstemmed | Preparation of isolated guard cells, containing cell walls, from Vicia faba. |
| title_short | Preparation of isolated guard cells, containing cell walls, from Vicia faba. |
| title_sort | preparation of isolated guard cells containing cell walls from vicia faba |
| url | https://doi.org/10.1371/journal.pone.0299810 |
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