Swimming attenuates tumor growth in CT-26 tumor-bearing mice and suppresses angiogenesis by mediating the HIF-1α/VEGFA pathway
Low physical activity correlates with increased cancer risk in various cancer types, including colorectal cancer (CRC). However, the ways in which swimming can benefit CRC remain largely unknown. In this study, mice bearing tumors derived from CT-26 cells were randomly divided into the control and s...
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| Format: | Article |
| Language: | English |
| Published: |
De Gruyter
2022-02-01
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| Series: | Open Life Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/biol-2022-0009 |
| _version_ | 1811203739409711104 |
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| author | Li Jiapeng Liu Liya Cheng Ying Xie Qiurong Wu Meizhu Chen Xiaoping Li Zuanfang Chen Haichun Peng Jun Shen Aling |
| author_facet | Li Jiapeng Liu Liya Cheng Ying Xie Qiurong Wu Meizhu Chen Xiaoping Li Zuanfang Chen Haichun Peng Jun Shen Aling |
| author_sort | Li Jiapeng |
| collection | DOAJ |
| description | Low physical activity correlates with increased cancer risk in various cancer types, including colorectal cancer (CRC). However, the ways in which swimming can benefit CRC remain largely unknown. In this study, mice bearing tumors derived from CT-26 cells were randomly divided into the control and swimming groups. Mice in the swimming group were subjected to physical training (swimming) for 3 weeks. Compared with the control group, swimming clearly attenuated tumor volume and tumor weight in CT-26 tumor-bearing mice. RNA sequencing (RNA-seq) identified 715 upregulated and 629 downregulated transcripts (including VEGFA) in tumor tissues of mice in the swimming group. KEGG pathway analysis based on differentially expressed transcripts identified multiple enriched signaling pathways, including angiogenesis, hypoxia, and vascular endothelial growth factor (VEGF) pathways. Consistently, IHC analysis revealed that swimming significantly downregulated CD31, HIF-1α, VEGFA, and VEGFR2 protein expression in tumor tissues. In conclusion, swimming significantly attenuates tumor growth in CT-26 tumor-bearing mice by inhibiting tumor angiogenesis via the suppression of the HIF-1α/VEGFA pathway. |
| first_indexed | 2024-04-12T03:00:15Z |
| format | Article |
| id | doaj.art-0f4fa4d093fa4f199acaec19172e0c49 |
| institution | Directory Open Access Journal |
| issn | 2391-5412 |
| language | English |
| last_indexed | 2024-04-12T03:00:15Z |
| publishDate | 2022-02-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Open Life Sciences |
| spelling | doaj.art-0f4fa4d093fa4f199acaec19172e0c492022-12-22T03:50:40ZengDe GruyterOpen Life Sciences2391-54122022-02-0117112113010.1515/biol-2022-0009Swimming attenuates tumor growth in CT-26 tumor-bearing mice and suppresses angiogenesis by mediating the HIF-1α/VEGFA pathwayLi Jiapeng0Liu Liya1Cheng Ying2Xie Qiurong3Wu Meizhu4Chen Xiaoping5Li Zuanfang6Chen Haichun7Peng Jun8Shen Aling9Department of Physical Education, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, ChinaAcademy of Integrative Medicine and Fujian Key Laboratory of Integrative Medicine in Geriatrics, Fujian University of Traditional Chinese Medicine, 1 Qiuyang, Minhou Shangjie, Fuzhou, Fujian 350122, ChinaAcademy of Integrative Medicine and Fujian Key Laboratory of Integrative Medicine in Geriatrics, Fujian University of Traditional Chinese Medicine, 1 Qiuyang, Minhou Shangjie, Fuzhou, Fujian 350122, ChinaAcademy of Integrative Medicine and Fujian Key Laboratory of Integrative Medicine in Geriatrics, Fujian University of Traditional Chinese Medicine, 1 Qiuyang, Minhou Shangjie, Fuzhou, Fujian 350122, ChinaAcademy of Integrative Medicine and Fujian Key Laboratory of Integrative Medicine in Geriatrics, Fujian University of Traditional Chinese Medicine, 1 Qiuyang, Minhou Shangjie, Fuzhou, Fujian 350122, ChinaAcademy of Integrative Medicine and Fujian Key Laboratory of Integrative Medicine in Geriatrics, Fujian University of Traditional Chinese Medicine, 1 Qiuyang, Minhou Shangjie, Fuzhou, Fujian 350122, ChinaAcademy of Integrative Medicine and Fujian Key Laboratory of Integrative Medicine in Geriatrics, Fujian University of Traditional Chinese Medicine, 1 Qiuyang, Minhou Shangjie, Fuzhou, Fujian 350122, ChinaProvincial University Key Laboratory of Sport and Health Science, School of Physical Education and Sport Sciences, Fujian Normal University, Fuzhou, Fujian 350007, ChinaAcademy of Integrative Medicine and Fujian Key Laboratory of Integrative Medicine in Geriatrics, Fujian University of Traditional Chinese Medicine, 1 Qiuyang, Minhou Shangjie, Fuzhou, Fujian 350122, ChinaAcademy of Integrative Medicine and Fujian Key Laboratory of Integrative Medicine in Geriatrics, Fujian University of Traditional Chinese Medicine, 1 Qiuyang, Minhou Shangjie, Fuzhou, Fujian 350122, ChinaLow physical activity correlates with increased cancer risk in various cancer types, including colorectal cancer (CRC). However, the ways in which swimming can benefit CRC remain largely unknown. In this study, mice bearing tumors derived from CT-26 cells were randomly divided into the control and swimming groups. Mice in the swimming group were subjected to physical training (swimming) for 3 weeks. Compared with the control group, swimming clearly attenuated tumor volume and tumor weight in CT-26 tumor-bearing mice. RNA sequencing (RNA-seq) identified 715 upregulated and 629 downregulated transcripts (including VEGFA) in tumor tissues of mice in the swimming group. KEGG pathway analysis based on differentially expressed transcripts identified multiple enriched signaling pathways, including angiogenesis, hypoxia, and vascular endothelial growth factor (VEGF) pathways. Consistently, IHC analysis revealed that swimming significantly downregulated CD31, HIF-1α, VEGFA, and VEGFR2 protein expression in tumor tissues. In conclusion, swimming significantly attenuates tumor growth in CT-26 tumor-bearing mice by inhibiting tumor angiogenesis via the suppression of the HIF-1α/VEGFA pathway.https://doi.org/10.1515/biol-2022-0009swimmingcolorectal cancerangiogenesishif-1α/vegfa pathway |
| spellingShingle | Li Jiapeng Liu Liya Cheng Ying Xie Qiurong Wu Meizhu Chen Xiaoping Li Zuanfang Chen Haichun Peng Jun Shen Aling Swimming attenuates tumor growth in CT-26 tumor-bearing mice and suppresses angiogenesis by mediating the HIF-1α/VEGFA pathway Open Life Sciences swimming colorectal cancer angiogenesis hif-1α/vegfa pathway |
| title | Swimming attenuates tumor growth in CT-26 tumor-bearing mice and suppresses angiogenesis by mediating the HIF-1α/VEGFA pathway |
| title_full | Swimming attenuates tumor growth in CT-26 tumor-bearing mice and suppresses angiogenesis by mediating the HIF-1α/VEGFA pathway |
| title_fullStr | Swimming attenuates tumor growth in CT-26 tumor-bearing mice and suppresses angiogenesis by mediating the HIF-1α/VEGFA pathway |
| title_full_unstemmed | Swimming attenuates tumor growth in CT-26 tumor-bearing mice and suppresses angiogenesis by mediating the HIF-1α/VEGFA pathway |
| title_short | Swimming attenuates tumor growth in CT-26 tumor-bearing mice and suppresses angiogenesis by mediating the HIF-1α/VEGFA pathway |
| title_sort | swimming attenuates tumor growth in ct 26 tumor bearing mice and suppresses angiogenesis by mediating the hif 1α vegfa pathway |
| topic | swimming colorectal cancer angiogenesis hif-1α/vegfa pathway |
| url | https://doi.org/10.1515/biol-2022-0009 |
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