Arabidopsis thaliana Plants Overexpressing the Barley Nicotinamine Synthase1 (HvNAS1) Gene Show Tolerance to Iron Deficiency
Iron (Fe) is an important trace mineral for plant development, and plants grown in Fe deficiency experience yield losses due to the leaf chlorosis. In addition to agronomic measures that can be taken to minimize these losses, new plant genotypes can be developed effectively through genetic engineeri...
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| Format: | Article |
| Language: | English |
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Turkish Science and Technology Publishing (TURSTEP)
2020-12-01
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| Series: | Turkish Journal of Agriculture: Food Science and Technology |
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| Online Access: | http://www.agrifoodscience.com/index.php/TURJAF/article/view/3974 |
| _version_ | 1797913801592406016 |
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| author | Emre Aksoy Amir Maqbool Buasimuhan Abudureyimu |
| author_facet | Emre Aksoy Amir Maqbool Buasimuhan Abudureyimu |
| author_sort | Emre Aksoy |
| collection | DOAJ |
| description | Iron (Fe) is an important trace mineral for plant development, and plants grown in Fe deficiency experience yield losses due to the leaf chlorosis. In addition to agronomic measures that can be taken to minimize these losses, new plant genotypes can be developed effectively through genetic engineering. While dicots such as Arabidopsis thaliana use a reduction-based strategy to uptake high amounts of iron from the rhizosphere, the chelation strategy has evolved in Gramineous plants including barley (Hordeum vulgare). In this study, barley NICOTIANAMINE SYNTHASE1 (HvNAS1) gene, which is responsible for the production of nicotianamine that can complex with iron, was cloned and expressed at a constitutive high level in Arabidopsis plants. The expression levels of Arabidopsis genes encoding for the proteins involved in iron uptake increased together with HvNAS1 in the T3 Arabidopsis plants. Moreover, the root lengths, root and stem fresh weights, ferric chelate reductase enzyme activities of the plants also increased in the transgenic Arabidopsis plants under Fe deficiency. In addition, significant increases in iron and zinc levels were determined in the roots and shoots of transgenic Arabidopsis plants. As a result, transgenic Arabidopsis plants overexpressing the barley HvNAS1 gene can take up more iron from the rhizosphere and carry this iron to the shoots. This study demonstrates the power of genetic engineering to develop Arabidopsis plants overexpressing the HvNAS1 gene and therefore tolerate iron deficiency. |
| first_indexed | 2024-04-10T12:17:27Z |
| format | Article |
| id | doaj.art-0f51e3122c3b4896a41bf0e2e6f07322 |
| institution | Directory Open Access Journal |
| issn | 2148-127X |
| language | English |
| last_indexed | 2024-04-10T12:17:27Z |
| publishDate | 2020-12-01 |
| publisher | Turkish Science and Technology Publishing (TURSTEP) |
| record_format | Article |
| series | Turkish Journal of Agriculture: Food Science and Technology |
| spelling | doaj.art-0f51e3122c3b4896a41bf0e2e6f073222023-02-15T16:15:36ZengTurkish Science and Technology Publishing (TURSTEP)Turkish Journal of Agriculture: Food Science and Technology2148-127X2020-12-018sp1707910.24925/turjaf.v8isp1.70-79.39741874Arabidopsis thaliana Plants Overexpressing the Barley Nicotinamine Synthase1 (HvNAS1) Gene Show Tolerance to Iron DeficiencyEmre Aksoy0Amir Maqbool1Buasimuhan Abudureyimu2Department of Agricultural Genetic Engineering, Faculty of Agricultural Sciences and Technologies, Niğde Ömer Halisdemir University, 51240 NiğdeDepartment of Agricultural Genetic Engineering, Faculty of Agricultural Sciences and Technologies, Niğde Ömer Halisdemir University, 51240 NiğdeDepartment of Agricultural Genetic Engineering, Faculty of Agricultural Sciences and Technologies, Niğde Ömer Halisdemir University, 51240 NiğdeIron (Fe) is an important trace mineral for plant development, and plants grown in Fe deficiency experience yield losses due to the leaf chlorosis. In addition to agronomic measures that can be taken to minimize these losses, new plant genotypes can be developed effectively through genetic engineering. While dicots such as Arabidopsis thaliana use a reduction-based strategy to uptake high amounts of iron from the rhizosphere, the chelation strategy has evolved in Gramineous plants including barley (Hordeum vulgare). In this study, barley NICOTIANAMINE SYNTHASE1 (HvNAS1) gene, which is responsible for the production of nicotianamine that can complex with iron, was cloned and expressed at a constitutive high level in Arabidopsis plants. The expression levels of Arabidopsis genes encoding for the proteins involved in iron uptake increased together with HvNAS1 in the T3 Arabidopsis plants. Moreover, the root lengths, root and stem fresh weights, ferric chelate reductase enzyme activities of the plants also increased in the transgenic Arabidopsis plants under Fe deficiency. In addition, significant increases in iron and zinc levels were determined in the roots and shoots of transgenic Arabidopsis plants. As a result, transgenic Arabidopsis plants overexpressing the barley HvNAS1 gene can take up more iron from the rhizosphere and carry this iron to the shoots. This study demonstrates the power of genetic engineering to develop Arabidopsis plants overexpressing the HvNAS1 gene and therefore tolerate iron deficiency.http://www.agrifoodscience.com/index.php/TURJAF/article/view/3974arabidopsis thalianaarpadayanıklılıkdemir eksikliğinikotinamin sentaz |
| spellingShingle | Emre Aksoy Amir Maqbool Buasimuhan Abudureyimu Arabidopsis thaliana Plants Overexpressing the Barley Nicotinamine Synthase1 (HvNAS1) Gene Show Tolerance to Iron Deficiency Turkish Journal of Agriculture: Food Science and Technology arabidopsis thaliana arpa dayanıklılık demir eksikliği nikotinamin sentaz |
| title | Arabidopsis thaliana Plants Overexpressing the Barley Nicotinamine Synthase1 (HvNAS1) Gene Show Tolerance to Iron Deficiency |
| title_full | Arabidopsis thaliana Plants Overexpressing the Barley Nicotinamine Synthase1 (HvNAS1) Gene Show Tolerance to Iron Deficiency |
| title_fullStr | Arabidopsis thaliana Plants Overexpressing the Barley Nicotinamine Synthase1 (HvNAS1) Gene Show Tolerance to Iron Deficiency |
| title_full_unstemmed | Arabidopsis thaliana Plants Overexpressing the Barley Nicotinamine Synthase1 (HvNAS1) Gene Show Tolerance to Iron Deficiency |
| title_short | Arabidopsis thaliana Plants Overexpressing the Barley Nicotinamine Synthase1 (HvNAS1) Gene Show Tolerance to Iron Deficiency |
| title_sort | arabidopsis thaliana plants overexpressing the barley nicotinamine synthase1 hvnas1 gene show tolerance to iron deficiency |
| topic | arabidopsis thaliana arpa dayanıklılık demir eksikliği nikotinamin sentaz |
| url | http://www.agrifoodscience.com/index.php/TURJAF/article/view/3974 |
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