NEW DATA ON THE POLYMORPHISM OF THE VOLGA POPULATION OF THE IMPERIAL EAGLE (AQUILA HELIACA, FALCONIFORMES, ACCIPITRIDAE) BASED ON THE RESULTS OF THE ANALYSIS OF MITOCHONDRIAL AND MICROSATELLITE DNA
Background. The analysis of the available data on the study of the polymorphism of the mtDNA control region in the Eurasian populations of burial eagles (1996-2020) indicates their genetic differentiation by geographical distribution. The periodic detection of new haplotypes indicates the relevanc...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Penza State University Publishing House
2021-09-01
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| Series: | Russian Journal of Ecosystem Ecology |
| Subjects: |
| _version_ | 1818833863167705088 |
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| author | M. D. Simakov M. V. Korepov S. A. Stryukov D. A. Korepova A. A. Kuz'min S. V. Titov |
| author_facet | M. D. Simakov M. V. Korepov S. A. Stryukov D. A. Korepova A. A. Kuz'min S. V. Titov |
| author_sort | M. D. Simakov |
| collection | DOAJ |
| description | Background. The analysis of the available data on the study of the polymorphism of the mtDNA control
region in the Eurasian populations of burial eagles (1996-2020) indicates their genetic differentiation by geographical
distribution. The periodic detection of new haplotypes indicates the relevance of such studies and insufficient
knowledge of this issue. The aim of the work is to clarify the level of polymorphism and identify specific
genetic features of the Volga population of the imperial eagle (A. heliaca) according to the analysis of the variability
of mitochondrial and microsatellite DNA. Materials and methods. The material for genetic research was new
collections (n = 22) of linn feathers of burial eagles collected in the Ulyanovsk region (n = 15), the Republic of Tatarstan
(n = 4), the Republic Mordovia (n = 2) and the Chuvash Republic (n = 1). DNA was isolated from feathers of
adult birds and the pulp and blood of chicks according to the standard method of phenol-chloroform extraction.
A fragment (345 pn) of the mtDNA control region (D-loop) and three tetranucleotide microsatellite repeats
(IEAAAG09, IEAAAG11, IEAAAG15) were used for genetic analysis. Fragments of the mtDNA control region (n = 22)
and microsatellite DNA (n = 171) were sequenced using an ABI 3500 sequencer (Applied Biosystems). Haplotypic
and nucleotide diversity were studied using the DnaSP 5.10.01 program. To reconstruct phylogenetic relationships,
Maximum Likelihood (ML) and Neighbor-Joining (NJ) methods were used in the MEGA X program. Microsatellite
genetic data was analyzed using the Arlequin 3.5.2 and Structure 2.3.4 programs. The obtained nucleotide sequences
of newly identified mtDNA haplotypes of the imperial eagle were deposited in GenBank NCBI under the
numbers OK504653 (haplotype RE) and OK504654 (haplotype RF). For statistical processing of the results, a significance
level of p < 0.05 was set for all tests. Results. Analysis of the distribution of mtDNA control region haplotypes
across 5 geographically isolated Imperial eagle breeding groups in the Volga region (ULR-NW – northwest of
the Ulyanovsk region, UlR-NE – northeast of the Ulyanovsk region, UlR–C – center of the Ulyanovsk region, UlR-S –
south of the Ulyanovsk region, RTat – Republic of Tatarstan) revealed their subdivision. When analyzing the average
numbers of nucleotide differences, the isolation of the breeding groups from the Republic of Tatarstan and
the Ulyanovsk region is noticeable. The average number of nucleotide differences (k) between all nest groups was
1.606 and was lower than this indicator when comparing the group from Tatarstan with the Ulyanovsk groups
(1.739). In the phylogenetic analysis, two haplogroups of the imperial eagle were identified. The first haplogroup
(AQH_1) is formed by mtDNA haplotypes characterized by one or two nucleotide substitutions. The second haplogroup
(AQH_2) consists of haplotypes having from 3 to 5 nucleotide substitutions. The results of the median test
also indicate the existence of 2 genetically close groups of sequences of a fragment of the mtDNA control region
corresponding to more or less altered haplotypes. The analysis of microsatellite DNA of geographically isolated
breeding groups of imperial eagles in the Ulyanovsk region revealed a low level of internal polymorphism. Conclusion.
The conducted studies have shown that the Volga population of the imperial eagle is genetically differentiated.
It consists of two subpopulations – Ulyanovsk and Tatarstan, characterized by specific mtDNA haplotypes.
These subpopulations are confined to the Volga and Bugulminsko-Belebeevskaya uplands. The analysis of microsatellite
DNA, as well as the reconstruction of evolutionary events related to the variability of mtDNA haplotypes show that the level of polymorphism of the Ulyanovsk subpopulation of imperial eagles is low, and the population
itself is fairly homogeneous. |
| first_indexed | 2024-12-19T02:25:41Z |
| format | Article |
| id | doaj.art-0f557077868f4544acb56a629ea8711a |
| institution | Directory Open Access Journal |
| issn | 2500-0578 |
| language | English |
| last_indexed | 2024-12-19T02:25:41Z |
| publishDate | 2021-09-01 |
| publisher | Penza State University Publishing House |
| record_format | Article |
| series | Russian Journal of Ecosystem Ecology |
| spelling | doaj.art-0f557077868f4544acb56a629ea8711a2022-12-21T20:39:55ZengPenza State University Publishing HouseRussian Journal of Ecosystem Ecology2500-05782021-09-01310.21685/2500-0578-2021-3-2NEW DATA ON THE POLYMORPHISM OF THE VOLGA POPULATION OF THE IMPERIAL EAGLE (AQUILA HELIACA, FALCONIFORMES, ACCIPITRIDAE) BASED ON THE RESULTS OF THE ANALYSIS OF MITOCHONDRIAL AND MICROSATELLITE DNAM. D. Simakov0M. V. Korepov1S. A. Stryukov2D. A. Korepova3A. A. Kuz'min4S. V. Titov5Penza State UniversityUlyanovsk State Pedagogical University named after I. N. UlyanovUlyanovsk Regional Museum of Local History named after I. A. GoncharovUlyanovsk Regional Museum of Local History named after I. A. GoncharovPenza State Technological UniversityPenza State UniversityBackground. The analysis of the available data on the study of the polymorphism of the mtDNA control region in the Eurasian populations of burial eagles (1996-2020) indicates their genetic differentiation by geographical distribution. The periodic detection of new haplotypes indicates the relevance of such studies and insufficient knowledge of this issue. The aim of the work is to clarify the level of polymorphism and identify specific genetic features of the Volga population of the imperial eagle (A. heliaca) according to the analysis of the variability of mitochondrial and microsatellite DNA. Materials and methods. The material for genetic research was new collections (n = 22) of linn feathers of burial eagles collected in the Ulyanovsk region (n = 15), the Republic of Tatarstan (n = 4), the Republic Mordovia (n = 2) and the Chuvash Republic (n = 1). DNA was isolated from feathers of adult birds and the pulp and blood of chicks according to the standard method of phenol-chloroform extraction. A fragment (345 pn) of the mtDNA control region (D-loop) and three tetranucleotide microsatellite repeats (IEAAAG09, IEAAAG11, IEAAAG15) were used for genetic analysis. Fragments of the mtDNA control region (n = 22) and microsatellite DNA (n = 171) were sequenced using an ABI 3500 sequencer (Applied Biosystems). Haplotypic and nucleotide diversity were studied using the DnaSP 5.10.01 program. To reconstruct phylogenetic relationships, Maximum Likelihood (ML) and Neighbor-Joining (NJ) methods were used in the MEGA X program. Microsatellite genetic data was analyzed using the Arlequin 3.5.2 and Structure 2.3.4 programs. The obtained nucleotide sequences of newly identified mtDNA haplotypes of the imperial eagle were deposited in GenBank NCBI under the numbers OK504653 (haplotype RE) and OK504654 (haplotype RF). For statistical processing of the results, a significance level of p < 0.05 was set for all tests. Results. Analysis of the distribution of mtDNA control region haplotypes across 5 geographically isolated Imperial eagle breeding groups in the Volga region (ULR-NW – northwest of the Ulyanovsk region, UlR-NE – northeast of the Ulyanovsk region, UlR–C – center of the Ulyanovsk region, UlR-S – south of the Ulyanovsk region, RTat – Republic of Tatarstan) revealed their subdivision. When analyzing the average numbers of nucleotide differences, the isolation of the breeding groups from the Republic of Tatarstan and the Ulyanovsk region is noticeable. The average number of nucleotide differences (k) between all nest groups was 1.606 and was lower than this indicator when comparing the group from Tatarstan with the Ulyanovsk groups (1.739). In the phylogenetic analysis, two haplogroups of the imperial eagle were identified. The first haplogroup (AQH_1) is formed by mtDNA haplotypes characterized by one or two nucleotide substitutions. The second haplogroup (AQH_2) consists of haplotypes having from 3 to 5 nucleotide substitutions. The results of the median test also indicate the existence of 2 genetically close groups of sequences of a fragment of the mtDNA control region corresponding to more or less altered haplotypes. The analysis of microsatellite DNA of geographically isolated breeding groups of imperial eagles in the Ulyanovsk region revealed a low level of internal polymorphism. Conclusion. The conducted studies have shown that the Volga population of the imperial eagle is genetically differentiated. It consists of two subpopulations – Ulyanovsk and Tatarstan, characterized by specific mtDNA haplotypes. These subpopulations are confined to the Volga and Bugulminsko-Belebeevskaya uplands. The analysis of microsatellite DNA, as well as the reconstruction of evolutionary events related to the variability of mtDNA haplotypes show that the level of polymorphism of the Ulyanovsk subpopulation of imperial eagles is low, and the population itself is fairly homogeneous.aquila heliacacontrol regionmtdnahaplotype diversityvolga region population |
| spellingShingle | M. D. Simakov M. V. Korepov S. A. Stryukov D. A. Korepova A. A. Kuz'min S. V. Titov NEW DATA ON THE POLYMORPHISM OF THE VOLGA POPULATION OF THE IMPERIAL EAGLE (AQUILA HELIACA, FALCONIFORMES, ACCIPITRIDAE) BASED ON THE RESULTS OF THE ANALYSIS OF MITOCHONDRIAL AND MICROSATELLITE DNA Russian Journal of Ecosystem Ecology aquila heliaca control region mtdna haplotype diversity volga region population |
| title | NEW DATA ON THE POLYMORPHISM OF THE VOLGA POPULATION OF THE IMPERIAL EAGLE (AQUILA HELIACA, FALCONIFORMES, ACCIPITRIDAE) BASED ON THE RESULTS OF THE ANALYSIS OF MITOCHONDRIAL AND MICROSATELLITE DNA |
| title_full | NEW DATA ON THE POLYMORPHISM OF THE VOLGA POPULATION OF THE IMPERIAL EAGLE (AQUILA HELIACA, FALCONIFORMES, ACCIPITRIDAE) BASED ON THE RESULTS OF THE ANALYSIS OF MITOCHONDRIAL AND MICROSATELLITE DNA |
| title_fullStr | NEW DATA ON THE POLYMORPHISM OF THE VOLGA POPULATION OF THE IMPERIAL EAGLE (AQUILA HELIACA, FALCONIFORMES, ACCIPITRIDAE) BASED ON THE RESULTS OF THE ANALYSIS OF MITOCHONDRIAL AND MICROSATELLITE DNA |
| title_full_unstemmed | NEW DATA ON THE POLYMORPHISM OF THE VOLGA POPULATION OF THE IMPERIAL EAGLE (AQUILA HELIACA, FALCONIFORMES, ACCIPITRIDAE) BASED ON THE RESULTS OF THE ANALYSIS OF MITOCHONDRIAL AND MICROSATELLITE DNA |
| title_short | NEW DATA ON THE POLYMORPHISM OF THE VOLGA POPULATION OF THE IMPERIAL EAGLE (AQUILA HELIACA, FALCONIFORMES, ACCIPITRIDAE) BASED ON THE RESULTS OF THE ANALYSIS OF MITOCHONDRIAL AND MICROSATELLITE DNA |
| title_sort | new data on the polymorphism of the volga population of the imperial eagle aquila heliaca falconiformes accipitridae based on the results of the analysis of mitochondrial and microsatellite dna |
| topic | aquila heliaca control region mtdna haplotype diversity volga region population |
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